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H Storch

Publications and source records attributed to H Storch.

At least 19 recordsLinked to original sources
PubMed2006

A prospective multicentre study on the safety of long-term intensive plasmapheresis in donors (SIPLA).

BACKGROUND AND OBJECTIVES: The safety of chronic intensive donor plasmapheresis has not been determined in large prospective studies examining dropout rates, dropout reasons and predictors of withdrawals. MATERIALS AND METHODS: Twenty-one plasma centres recruited 3783 donors who were switched from a moderate to an intensive plasmapheresis programme and observed over a 3-year period. Individuals weighing < 70 kg and > or = 70 kg donated 750 ml and 850 ml of plasma per session, respectively. The maximum of annual donations was limited to 60. Total serum protein (TSP) and haemoglobin (Hb) or haematocrit (Hct) were determined at each donation, and immunoglobulin G (IgG) at every fifth donation. Dropout rates, dropout reasons and potential predictors of withdrawal were analysed. RESULTS: Dropouts were predominantly due to socioeconomic (49.2% of all donors) or medical reasons not related to plasma donations (10.4% of all donors). Sixteen per cent of donors dropped out when IgG, TSP or Hb levels fell below threshold values. Severe clinical adverse events related to plasmapheresis were observed in five subjects. The incidence in severe cardiovascular diseases was lower in donors than in the general population. The risk factors that led to dropping out as a result of low IgG, TSP or Hb levels included younger age, female gender, low initial IgG levels and a high donation frequency. Neither body weight nor the amounts of plasma donated per kilogram of body weight per session were associated with ceasing due to medical reasons, whether related or unrelated to plasma donations. Females and males within the respective lowest body weight category were not at higher risk of dropping out. CONCLUSION: Long-term intensive donor plasmapheresis under conditions investigated in this study is safe. All donors weighing > or = 70 kg are safely able to donate 850 ml of plasma in each session up to 60 times per year, provided that they are carefully monitored.

Adult↗
PubMed2004

The impact of different intensities of regular donor plasmapheresis on humoral and cellular immunity, red cell and iron metabolism, and cardiovascular risk markers.

BACKGROUND AND OBJECTIVES: Major studies are still lacking on the impact of differing intensities of long-term donor plasmapheresis, not only on total serum protein, albumin and immunoglobulin G (IgG), but also on humoral and cellular immunity, red cell and iron metabolism, and biochemical cardiovascular risk markers. MATERIALS AND METHODS: Three groups of donors, comprising 483 individuals undergoing differing intensities of long-term serial plasmapheresis, were entered into a cross-sectional study. A fourth control group consisted of 100 non-donors. In addition to measuring total protein, albumin and IgG levels, we determined parameters of humoral and cellular immunity, red cell and iron metabolism and recognized biochemical cardiovascular risk factors. RESULTS: The median annual net amount of plasma donated by the three donor groups was 37, 16 and 10 l, respectively (P < 0.0001). Donors had significantly lower total serum protein, albumin and IgG levels than non-donors (P < 0.0001), but the intensity of plasmapheresis had no influence on those parameters. Like non-donors, all plasma donors had normal humoral and cellular immunity. No increased rates of iron store depletion were observed in the three groups of plasma donors. Plasma donors were not at increased cardiovascular risk. CONCLUSIONS: Regular donor plasmapheresis of up to 45 l of plasma per year appears to be as safe as more moderate plasmapheresis programmes, with respect to the parameters analysed in this study. Individuals donating under these conditions did not develop impaired humoral and cellular immunity, iron store depletion, or increased cardiovascular risk with regard to established biochemical risk markers. Prospective studies are required to determine more exactly than in retrospective analyses the reasons why donors withdraw from plasmapheresis programmes.

Adult↗
PubMed1997

[Plasma donor screening and product safety].

In addition to the specific virus reduction and inactivation procedures during the manufacturing process, the thorough selection of the source material is an essential factor for the product safety of plasmaderivatives. Screening of donors, inventory hold, and plasma pool testing by PCR are important aspects in raising the safety margin. Between January 1, 1994 and June 30, 1996, 576,673 plasma donations were collected in the German and Austrian plasmapheresis centers of IMMUNO. Incidence rates for viral markers (confirmatory tests) as antibodies against HIV, HCV, and HB surface antigen (HBsAg) were calculated as 0.35, 0.87, and 0.87 per 10(5) donations, respectively. Due to look-back, 1.33% of the donations were eliminated between January and October 1995. In 32% of the donations being rejected in the time of inventory hold in case of elevated alaninamino-transferase (ALT) of the donors, HCV genomes were shown which emphasizes the importance of this surrogate marker. Out of 1,240 pilot pools tested by quality assured PCR (IQ-PCR) being performed by IMMUNO since October 1995, in 4% of cases HCV and in 0.2% HBV genomic equivalents were shown. However, genomes of HIV were not found. The measures aiming at a safe plasma pool are part of IMMUNO's safety concept which is confirmed by the fact that there was no transmission of hepatitis or AIDS viruses by IMMUNO products, since virus inactivation procedures have been established.

Austria↗
PubMed1993

[Recombinant plasma proteins for therapeutic use--status and developmental trends].

The enormous progress made in biotechnology and purification of plasma proteins (pp) and the demands to avoid risks of transmitting HIV, hepatitis and other virus infections by these have resulted in the development of numerous recombinant human (rh) pp, which are now about to be used as replacement therapy in transfusion medicine. Human rh albumin has been used in clinical trials last year, a competition to serum albumin can be expected in the next time. During the last decade, the genes or cDNA have been cloned and characterized for all relevant pp involved in blood coagulation. Beside the rh factor VIII (rh FVIII) which has been introduced clinically in 1991, the rh FVIIa is under investigation in patients with hemophilia A and inhibitors. After establishing of rhFIX in triple transgenic mice, the industrial potential will be evaluated in terms of scale up culturing and production. The valuation of advantages and drawbacks of the current rh pp in comparison to conventional pp will have to be determined in the last decade of our century.

Amino Acid Sequence↗
PubMed1990

[Thrombogenicity of prothrombin complex concentrates].

PCC's were isolated either by adsorption to DEAE-Sephadex A-50 (particle size 40-120 microns; Pharmacia Uppsala) or by adsorption to Molselect DEAE-50 (particle size 100-320 microns; Reanal Budapest) from human citrated plasma after separation of factor VIII by cryoprecipitation. The two products obtained (without heparin) were both compared by in vitro (NAPTT, TGt50) and in vivo (venous stasis) model in rats acc. to WESSLER) tests. The agent prepared by adsorption to DEAE-Sephadex A-50 showed a significantly higher thrombogenicity than that prepared by Molselect DEAE-50. ED50 values for thrombus formation to amounted 15 Factor IX U/kg for the first product and to 210 factor IX U/kg for the second.

Blood Coagulation↗
PubMed1989

Quantitation of the relationship between tester cell number inoculated and SOS-inducing potency of 4-nitroquinoline-1-oxide (4-NQO) in an automated version of the SOS chromotest.

The SOS chromotest is a simple quantitative short-term bacterial assay for the detection of genotoxic activity of pure compounds or complex samples. On the basis of consecutive experiments aimed at demonstrating the relationship between the inoculum size and the outcome of the test using 4-nitroquinoline-1-oxide (4-NQO) as model genotoxin. It is shown that within the suitable range of the cell number there is a negative correlation between the number of tester cells and test sensitivity. Moreover, it could be demonstrated that the peak of the dose response curve, i.e., the maximal induction factor, is systematically influenced by the actual value of the ratio of beta-galactosidase to alkaline phosphatase enzyme activities at a 4-NQO concentration of zero. Last but not least, some simple statistical data describing the performance of the automated version of the SOS chromotest are also given.

4-Nitroquinoline-1-oxide↗
PubMed1989

[Infrared thermovision findings in a patient with infiltrating orbitopathy].

The use of infrared thermovision to examine a patient with infiltrative orbitopathy and combined immunodeficiency is described. In addition to a thermic quantification of infiltrated areas of the eyes and face, it proved possible, with dynamic measurement, to define exactly the extent of pathologically increased temperatures. Other applications of infrared thermovision in ophthalmology are discussed.

Adult↗
PubMed1989

Gas chromatographic-mass spectrometric investigation of four commercial pristane (2.6.10.14-tetramethylpentadecane) preparations used for plasmacytoma induction in BALB/c mice.

The most widely studied model of plasmacytomagenesis is the induction of plasmacytomas by intraperitoneal injections of pure alkanes such as pristane (2.6.10.14-tetramethylpentadecane). The presence of small amounts of genotoxic contaminants, that could contribute to the complex oncogenic process, in commercial pristane preparations has not been completely ruled out. Therefore, we conducted a chemical analysis of different pristane preparations applying a combined GC/MS technique, but were not capable of identifying any obvious or putative genotoxic/cancerogenic contaminants.

Animals↗
PubMed1989

[Histologic studies of paraproteinemic nephropathy in BALB/c mice with mineral oil-induced plasmacytoma].

In histological examinations of kidneys from BALB/c mice with induced plasmocytoma (n = 75) the well known morphological correlates of paraproteinemic nephropathy were found, but further changes like reactive tubular giant cells and interstitial sclerosing processes have been missing. Furthermore, in the severity of nephropathy no correlation were found between the level of paraprotein in serum and/or ascites and the duration of the disease. This may be an indirect sign of an individual nephrotoxicity of the Bence-Jones proteins.

Animals↗
PubMed1989

[Selective IgA defect. Clinical importance for stomatology].

The clinical picture of 6 women suffering from selective IgA immunodeficiency were studied in a 2-13 years lasting period of immunological outdoor patients care. Despite of respiratory infections, otitis media, sinusitis, aphtosis and exogenous allergy the first detection of IgA deficiency was delayed til adulthood (mean age 42 years). A case of a 22 years old women with complicated fistula after molar tooth extraction showed the problems of dentistry in such diagnosis. No significant deviation concerning periodontitis and caries has been demonstrated in all patients. However, the rate of IgA deficiency demands basic knowledge about diagnosis, prevention of local and systemic complications in this disease by all practising dentists.

Adult↗
PubMed1988

SOS chromotest, a quantitative short-term bacterial assay for the detection of genotoxic compounds in an automated version adapted to Bioscreen Analyzer System.

The SOS chromotest is a simple quantitative short-term bacterial assay for the detection of genotoxic activity of pure chemicals or complex samples. The test is based on the measurement of the induction of the SOS response by xenobiotics which cause damage in replicating or non replicating DNA. The assay has been originally developed as a test-tube method but has recently been modified and extensively evaluated (Quillardet and Hofnung 1985, Quillardet et al. 1985). In an attempt to automate the SOS chromotest a manual test procedure based on microtiter plates (Orgenics Ltd. 1986) has been further adapted to Bioscreen analyzer system (Labsystems OY, Helsinki, Finland). In this application the test is controlled by a self-contained, interactive programme and makes use of a kinetic measure principle for quantifying the enzymatic activities to be evaluated. The present experiences with the automated version of the SOS chromotest indicate its usefulness as primary screening method or part of a battery of bacterial short-term tests for genotoxins and point out its remarkable practical advantages.

4-Nitroquinoline-1-oxide↗
PubMed1988

Genotoxicity assessment of the plasmacytomagenic agent pristane (2.6.10.14-tetramethylpentadecane) and four related alkanes by the SOS chromotest.

The most extensively studied model of plasmacytomagenesis is the induction of plasmacytomas in BALB/c mice by i.p. injections of mineral oil or, chemically more defined, by several branched alkanes such as pristane (2.6.10.14-tetramethylpentadecane), phytane (2.6.10.14-tetramethylhexadecane), and 7-n-hexyloctadecane. The available evidence suggests that the primary biologic action of these plasmacytomagenic agents is to induce the formation of a chronic granulomatous tissue, the histological matrix of plasmacytoma development. However, certain genotoxic effects caused by the presence of these substances can not be ruled out a priori. Pristane, 2-methyldodecane, and 1.3-di-tert-butyl-5-methyl-cyclohexane as well as perhydroanthracene and hexahydrodibenzsuberane were proofed as potential genotoxic agents by the SOS chromotest, a quantitative bacterial colorimetric assay for genotoxins. The substances tested did not express any sign of genotoxicity, but exerted toxic effects to the E. coli tester strain.

Alkanes↗
PubMed1987

[Immunologic studies using the leukocyte adherence inhibition test in the diagnosis and postoperative follow-up of breast cancer].

37 women with histologically proved carcinoma of the breast as well as 3 control groups (benign breast diseases, other carcinomas, benign surgical diseases) were investigated by means of the leucocyte adherence inhibition test. In the primary diagnostics of the carcinoma of the breast the specificity was 56%, the sensitivity 70%. Follow-up studies on the conditions of a district hospital over a period of 2 years showed a different reactivity which is suitable for the evaluation of recurrence and immunological tumour-host relations.

Adult↗
PubMed1987

Induction of plasma cell tumours with 2,6,10,14-tetramethylpentadecane (pristane) and paraffin oil (paraffinum perliquidum) in BALB/c mice simultaneously exposed to sustained antigenic stimulation with bovine serum albumin (BSA).

Plasmacytomas were readily induced in BALB/c mice by three bimonthly i.p. injections of 0.5 ml pristane or paraffin oil. Additionally, two groups of mice were simultaneously given a prolonged antigenic stimulation with bovine serum albumin (BSA). The tumour incidence found in mice treated with pristane and BSA (75%) was higher than the plasmacytoma incidence in mice subjected to paraffin oil and BSA inoculations (53.7%, p = 0.05). The sustained antigenic stimulation with BSA had no effect on cumulative plasmacytoma incidence.

Animals↗
PubMed1986

[Diagnosis and evaluation of monoclonal immunoglobulins in the aged with reference to plasmacytoma].

A study on the epidemiological situation of the appearance of monoclonal immunoglobulins (mIg) in the district of Leipzig is presented. 443 accidentally selected test persons from old people's and nursing homes of a dispensary consulting hour and a ward for diagnostics in internal medicine at the age of more than 50 years were examined. Agargel electrophoresis and BSR, the usual diagnostic follow-up methods for the proof of mIg, were used. Depending upon age 88 examined patients showed increasingly extragradients (EG), 21 of them could all be identified as mIg. Significant sex differences after the 74th year of age refer to an at this time more distinct disturbance of the B-cell regulation in males. One third of the test persons had transitory mIg, among the persisting ones after all two patients with multiple myeloma were found. While the BSR is not suitable as screening method, the agargel electrophoresis (sensitivity and specifity in each case 84%, positive predictive value 24%) in the present prevalency of 4.7% is recommended for the detection of this phenomenon. The clinical consequences require the formation of risk groups, their mass examination and the aimed control in immunologically and haematologically orientated centres.

Aged↗