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Biomedical subjects

H Storz

Publications and source records attributed to H Storz.

At least 19 recordsLinked to original sources
PubMed1990

Hemocyanins in spiders, XXIII. Complete amino-acid sequence of subunit a of Eurypelma californicum hemocyanin.

The complete amino-acid sequence of subunit a of the hemocyanin of the tarantula Eurypelma californicum was determined by manual sequencing. By limited chymotrypsinolysis, subunit a is split into two fragments of 25 kDa and 40 kDa, respectively, only one single peptide bond being attacked. The whole chain contains 15 methionine residues, after cyanogen bromide cleavage, 15 peptides were identified indicating that one residue (Met85) was not split by the cyanogen bromide reaction. For subcleavages, trypsin, chymotrypsin, Staphylococcus aureus proteinase, and Astacus fluviatilis proteinase were employed. The total chain length comprises 627 amino-acid residues, carbohydrate side chains were not found.

Amino Acid Sequence↗
PubMed1990

[Age-dependent sensitivity of human lymphocytes to the immunomodulating effect of bovine and human diacetyl splenopentin].

Both newborns and elderly adults suffer from physiological immunodeficiency. The molecular mechanisms responsible for this immunodeficiency are currently investigated by many laboratories. The aim of our investigations was to answer the question wether these immunodeficiencies could be influenced by bovine and/or human diacetyl-splenopentin, two newly developed immunostimulatory peptides. The in vitro effects of these peptides were studied using the lymphocyte transformation test and the detection of the immunoglobulin production (IgG, IgM) by lymphocytes. Thymopentin was used as standard for these investigations. The age dependence of lymphocyte sensitivity was estimated using cells of the following groups of blood donors: newborns (cord blood); young donors (20-30 yr); old donors (over 70 yr). All peptides were shown to have the same effects. The stimulated lymphocyte proliferation (PHA, anti-CD3) was inhibited in young donors and further increased in old donors. There was no influence in the case of newborns. The biological activity of human diacetyl-splenopentin was shown to be higher in comparison with bovine diacetyl-splenopentin.

Adult↗
PubMed1990

[Disease-specific and non-specific seasonal parameter changes in hayfever patients].

10 patients have been treated by subcutaneous injections of placebo three times weekly for 4 weeks within a phase-I trial of BCH-069. Immunological, hematological and biochemical parameters were observed at different times: preseasonal, seasonal and postseasonal. --Some new observations are reported, which are induced by the seasonal inflammation. For example there was a strong increase of C-reactive protein and also an increase in the triglyceride level. --The parameters could be useful for trials of new antiallergic drugs in hay fever in the future.

Adjuvants, Immunologic↗
PubMed1989

[Status and developmental trends in the use of fluorescence microscopy studies in microbiology].

There are many applications for fluorescence microscopy in the field of microbiology for diagnostic and scientific purposes. Autofluorescence as well as secondary fluorescence induced by staining of specimen with fluorochromes or with fluorochrome labeled antibodies are used for detection and differentiation of microorganisms. Small demands for object preparation and short test times are advantages for screening tests. During the last time considerable progresses in methods and technical equipments are noticeable and offer numerous new applications for fluorescence microscopy. New microscopes show clearly increased intensity of excitation. This requires a selection of object slides and cover glasses with lowest autofluorescence, blocking of fading by addition of PPD to embedding medium and a better adaptation of filters for excitation and fluorescence to the characteristics of fluorochromes. Multiple fluorochroming and combined application of fluorescence and alternative contrast techniques such as phase contrast are of rising importance in practice.

Bacteria↗
PubMed1986

Double staining technique using a combination of indirect and direct immunofluorescence with monoclonal antibodies.

A simple method for double staining by immunofluorescence is described. If for double staining using monoclonal antibodies of the same species only one antibody is conjugated with FITC or TRITC, a combination of indirect and direct immunofluorescence is possible. For cell staining the following incubation steps are carried out: Monoclonal antibody I (unlabelled, mouse), anti-mouse immunoglobulin serum FITC- or TRITC-conjugated, normal mouse serum for blocking of free binding sites of the anti-mouse immunoglobulin, and monoclonal antibody II (mouse) which is conjugated with an alternative fluorochrome. The use of this method is demonstrated for investigation of single cell suspensions (performed as a slide test) and of cryostat sections.

Animals↗
PubMed1984

Photomicrography of weakly fluorescent objects--employment of p-phenylene diamine as a blocker of fading.

By adding p-phenylene diamine (PPD) to the embedding medium, the fading of fluorescent objects labeled with FITC or mithramycin is substantially reduced. Thus, a multiple quantity of light, as compared to without additive, may be obtained from the objects and so photomicrography be improved or made possible at all. For microfluorometry as well as for subjective fluorescence microscopy the employment of PPD is not very helpful.

Fluorescent Antibody Technique↗
PubMed1983

[Subacute idiopathic polymyositis with rare antiribosomal antibodies in the serum - clinical course during immunosuppressive long-term therapy].

In a 66-year-old woman a polymyositis with subacute course was observed. The proof of very rare antiribosomal antibodies with a high titre on the serum was remarkable. Only after a cytostatic immunosuppressive therapy a clinical improvement developed, parallel to which there was a tendency to normalisation of immunological and enzyme-pathological parameters. The decrease of the titres of the autoantibodies is evaluated as indicators of the effectivity of the medicamentous immunosuppression and using the special literature is discussed in its importance also concerning the possibilites of the control of the clinical course.

Aged↗
PubMed1983

[Instrumentation technology problems in immunofluorescence microscopy].

The influence of construction units of the microscope such as filters, lamps and objectives upon fluorescent intensity and contrast is demonstrated. Further, the bleaching of fluorescence and the possibilities of contrast illumination are dealth with. Of crucial importance to the evaluation of the fluorescent images is their contrast rather than the intensity of fluorescence. Narrow band excitation is beneficial and brightening of the background, strong bleaching as well as too great a secondary magnification are disadvantageous to the contrast.

Fluorescent Antibody Technique↗
PubMed1983

[Problems in the characterization of autoantibodies in patients' sera by microfluorometry as exemplified by antimitochondrial antibodies].

Attention is called to various problems of subjective differentiation of fluorescence patterns for demonstrating autoantibodies by the indirect immunofluorescent technique. For the objective measurement of the fluorescent intensity, the main problem is the dependence of the measuring results upon the thickness of the tissue sections. The determination of the relationships of the fluorescent intensity in one preparation is independent on the thickness of the section. Using 25 patient sera with antimitochondrial antibodies, it could be shown that the relationship of the fluorescent intensity of various positively reacting tissue areas is not constant, but changes considerably depending on the concentration of the autoantibody in the test solution. Thus, differentiation of subgroups of antimitochondrial antibodies cannot be made using one serum dilution.

Antibody Specificity↗
PubMed1983

Marker and functional characterization of immunoregulatory cells in rheumatoid arthritis.

In 34 patients with rheumatoid arthritis we determined the regulatory index OKT 4: OKT 8 and the suppressor index delta 24: delta 0. There is no correlation between the results of the detection of subpopulations with monoclonal antibodies and of the functional test to determine the suppressor activity. The enhanced mitogen-induced proliferation after 24-h preincubation is regularly associated with a rise in the regulatory index; however, without convincing quantitative correlation. It can be concluded, that only a small portion of the cell population identified by monoclonal antibodies OKT 4 and OKT 8 exerts helper or suppressor activities. At present, identification of regulatory T-cell subpopulation should be complemented by functional tests.

Antibodies, Monoclonal↗
PubMed1983

Hemocyanins in spiders, XIX. Complete amino-acid sequence of subunit d from Eurypelma californicum hemocyanin, and comparison to chain e.

The complete primary structure of subunit d of the hemocyanin from the tarantula Eurypelma californicum was determined by manual micro sequencing. Subunit d of Mr = 73000 is split about in the middle of the chain during limited trypsinolysis, only one single bond being attacked. The whole chain contains 14 methionine residues and after cyanogen bromide cleavage 15 peptides could be isolated by gel and ion exchange chromatography and high pressure liquid chromatography. The cyanogen bromide peptides and the large (Mr = 34000 and 37000, respectively) fragments resulting from limited trypsinolysis, were further cleaved with trypsin, chymotrypsin, Staphylococcus aureus proteinase, formic acid, and Astacus fluviatilis proteinase, the latter being very useful in obtaining certain overlapping peptides. The total chain length is 627 residues. Carbohydrate side chains were not found. The sequence is discussed with respect to the gross physical properties of the subunit, to homologies with subunit e and the cleavage specifities of the enzymes employed.

Amino Acid Sequence↗
PubMed1982

Investigations of fading of immunofluorescence objects.

Fading of immunofluorescent objects was influenced by attenuation of fluorescence intensity whether by attenuation of excitation, post-fixation or counterstaining. In comparison with the wide-band excitation, narrow-band excitation had an influence on the fading rate via the attenuation of the excitation intensity only. Compared with aqueously immersed objects we obtained only half of the intensity and a quite stronger decrease of fluorescence with dry objects or objects embedded with fast-hardening medium. Addition of thiosulphate to embedding medium had no effect on fading. In opposite literature we found in our immunofluorescence specimen after addition of dithionite an extremely increased fading rate.

Animals↗
PubMed1980

[Standardization of indirect immunofluorescence for detection of autoantibodies (author's transl)].

Seven laboratories made a chessboard titration with a serum containing antimitochondrial antibodies and two conjugates labeled with fluorescein isothiocyanate. The variation of the plateau titer and the titer of the serum as well as the plateau-end point was slight with the exception of one case. The standardization of indirect immunofluorescence needs a close contact between the laboratories, the availability of reference sera and conjugates, and similar microscopic equipment.

Autoantibodies↗