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H T Delves

Publications and source records attributed to H T Delves.

At least 91 records · Page 5Linked to original sources

Evaluation of a new mineral and trace metal supplement for use with synthetic diets.

In an earlier study (Alexander et al., 1974) it was shown that the mineral mixture used in association with a synthetic diet was deficient in zinc, copper, iron, and manganese. A new mixture containing appropriately increased amounts of these trace metals has now been evaluated by means of metabolic balance studies carried out on children with phenylketonuria. This new mixture was shown to be satisfactory for zinc, copper, and iron, but minor changes have been recommended for manganese.

Child↗

The microdetermination of copper in plasma protein fractions.

Electrophoresis on cellulose acetate membranes and electrothermal atomization for atomic absorption spectrophotometry have been combined to allow the determination of copper in protein fractions separated from only 2 mul volumes of serum. The sensitivity of the method is 5.2 X 10(-11) g of copper for 1% absorption at 324.7 nm, and the precision of replicate analyses at the 1.7-ng level is 0.086 (Relative Standard deviation). The mean recovery of copper from the alpha2-protein fraction of a serum sample was 94%. The method has been applied to a study of the changes in the copper content of the various protein fractions of sera from patients with Menkes' syndrome.

Blood Proteins↗

Semi-automatic determination of lead in whole blood.

The procedure developed by Browett and Moss (1964) for the semi-automatic determination of the lead content of urine has been adapted for the determination of lead in blood. Determinations are normally carried out in duplicate on 2.0 ml. samples of whole blood and the minimum sample size is 0.5 ml. The organic substances present in blood are destroyed by a manual wet-oxidation procedure and the lead is determined colorimetrically as lead dithizonate using a Technicon AutoAnalyzer. The lower limit of detection, expressed as three times the standard deviation of the blank value, is 5 mug. Pb/100 ml. blood. The standard deviation of the method in the upper range of normal blood lead level of 30 mug. Pb/100 ml. blood (Moncrieff, Koumides, Clayton, Patrick, Renwick, and Roberts, 1964), is +/- 3 mug. Pb/100 ml. blood. Ten samples per hour may be estimated in duplicate.

Autoanalysis↗