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H Taga

Publications and source records attributed to H Taga.

41 records · Page 3Linked to original sources
PubMed1983

Immunohistochemical study of alpha-fetoprotein in rat embryos during ontogenesis.

In order to clarify the dynamics of alpha-fetoprotein (AFP) during ontogenesis, the appearance and localization of AFP and albumin in rat embryos were immunohistochemically investigated. AFP began to appear in the embryos on day 9 in some of the cells constituting the embryonic ectoderm. From day 10, both AFP and albumin became strongly stained in the yolk sac. The formation of liver anlage started on day 11; here AFP alone was clearly apparent in the primitive hepatocytes and also in some other cells, such as somites, or those of the central nervous system. After further development of the embryos, AFP, albumin, and transferrin were detected by means of serial section examination in the same hepatocytes. Besides being found in the liver, AFP began to be stained in the dorsal pancreas and mesonephric vesicles on day 12, in the stomach on day 13, and in the lung anlage and metanephros on day 14. Albumin was also localized in the same sites in these tissues, but the intensity of albumin staining was not as strong as that of AFP. Therefore, we assume that AFP plays an important role in the rapid growth of rats during the early gestational stages.

Albumins↗
PubMed1983

The effect of active immunization of rats with heterologous alpha-fetoprotein upon hepatocarcinogenesis induced by 3'-methyl-4-dimethylaminoazobenzene.

Immunization of rats with a purified mouse alpha-fetoprotein (AFP) suspended in Freund's complete adjuvant resulted in the production of antibodies that could precipitate both mouse and rat AFPs. A group of rats was immunized first and then fed a diet containing 0.06% 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) for 10 weeks. In these rats the elevation of serum AFP as well as the development of hepatoma were markedly inhibited. Another group of rats was immunized after feeding the diet containing 3'-Me-DAB for 10 weeks. The development of hepatoma and production of serum AFP were also suppressed. In both groups the life span of the rats was prolonged by the immunization.

Animals↗
PubMedDate not supplied

Inhibition by 1-(2-tetrahydrofuryl)-5-fluorouracil in combination with uracil of hepatocarcinogenesis induced by 3'-methyl-4-dimethylaminoazobenzene in rats.

It is known that a high incidence of hepatocellular carcinoma in rat liver can be induced by 3'-methyl-4-dimethylaminoazobenzene (3'-MeDAB). The administration of 3'-MeDAB in combination with 1-(2-tetrahydrofuryl)-5-fluorouracil and uracil (UFT) delayed the appearance of oval cells and the formation of hyperplastic nodules, which were observed in the liver from 3 and 5 weeks, respectively, after the onset of 3'-MeDAB feeding, and also delayed the transient increase of serum alpha-fetoprotein level, which transiently peaked at 5 weeks, and completely suppressed the transient increase of tissue thymidylate synthetase activity, but not thymidine kinase, which were induced by 3'-MeDAB at 5 weeks, and finally reduced markedly the incidence of hepatocarcinomas. These results indicate that the suppression of de novo synthesis in pyrimidine metabolism prevents hepatocarcinogenesis.

Animals↗
PubMedDate not supplied

Synergistic effect of oral UFT in combination with cisplatin on DNA synthesis in rat hepatocarcinomas.

We investigated the synergistic efficacy of CDDP and UFT, an oral anticancer derivative of 5-fluorouracil, on DNA synthesis of 3'-MeDAB-induced hepatocarcinomas in rats. Chronic treatment with CDDP slightly reduced tissue activities of thymidylate synthetase and thymidine kinase in the hepatocarcinomas, and, moreover, simultaneous administration of a low dose of UFT with CDDP markedly suppressed both enzyme activities, i.e., UFT and CDDP showed a potent synergism on DNA synthesis in hepatocarcinomas.

Administration, Oral↗