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Biomedical subjects

H Tamada

Publications and source records attributed to H Tamada.

At least 73 records · Page 4Linked to original sources
PubMed1993

[Fat necrosis arising in the perinerhrium].

The patient was a 74-year-old woman. During the investigation for abdominal pain, a mass lesion was detected adjacent to the left kidney using ultrasound. The left radical nephrectomy was performed under a clinical diagnosis of liposarcoma. Histological examination revealed fat necrosis with hemorrhage arising in the perinephrium. No tumor cells were confirmed microscopically. We reviewed the literature, but there found, no case reports of fat necrosis arising in the perinephrium, except for reports in rats or cows.

Aged↗
PubMed1992

Zinc-deficient diet impairs adaptive changes in the remaining intestine after massive small bowel resection in the rat.

An investigation was conducted on the influence of the presence of zinc in an elemental diet on the mucosa of residual intestine after massive small bowel resection. A total of 34 male Sprague-Dawley rats were divided into five groups: control animals (n = 10) were killed after overnight fasting; a second group (n = 14) underwent massive small bowel resection preserving 10 cm of terminal ileum, and the third group (n = 10) underwent sham operation. Animals in the second and third groups were fed either a commercially available elemental diet or a zinc-deficient diet for 2 weeks; they were then killed. In animals receiving the zinc-deficient diet, a significant decrease (P < 0.05) was noted in plasma zinc and total protein, and in mucosal wet weight (duodenum), thickness (duodenum and ileum), and protein (duodenum) and DNA (duodenum) content. Mucosal sucrase and maltase specific activities in the duodenum and ileum fell but diamine oxidase levels did not. These results suggest that zinc plays an important role in intestinal adaptation in the rat, and indicate that this trace element is essential for intestinal mucosal preservation in this animal.

Adaptation, Physiological↗
PubMed1992

Expression of insulin-like growth factor-I (IGF-I) and its receptor in the peri-implantation mouse uterus, and cell-specific regulation of IGF-I gene expression by estradiol and progesterone.

This study describes the expression of insulin-like growth factor-I (IGF-I) and IGF-I receptor (IGF-IR) genes in the mouse uterus during the peri-implantation period (Days 1-6 of pregnancy), as well as effects of estradiol (E) and progesterone (P) on cell-specific IGF-I gene expression in the uterus of the ovariectomized adult mouse. Northern blot analysis showed that IGF-I mRNA levels were low but readily detectable in the uterus on Day 1 of pregnancy and steadily increased, reaching high levels just before (Day 4) and after initiation of implantation (Days 5 and 6). In general, IGF-IR transcripts were present in low abundance in uterine RNA throughout the peri-implantation period. However, six sizes of uterine IGF-IR transcripts were detected, and the relative abundance of two of these transcripts varied significantly during the peri-implantation period. Cell-specific expression of the IGF-I gene was examined by in situ hybridization to mRNA and immunohistochemical detection of protein. The results indicated that the synthesis of IGF-I on Days 1 and 2 was most predominant in glandular and luminal epithelial cells. However, on Days 3 and 4, stromal cells, and on Days 5 and 6, decidual cells appeared to be the predominant sites of synthesis of this growth factor. Uterine IGF-I gene expression was stimulated by ovarian steroids. Northern blot analysis showed that IGF-I transcripts were rare in the ovariectomized adult mouse uterus, but an injection of P and/or E caused a rapid accumulation of these transcripts. Analysis of the cell-specific expression of uterine IGF-I showed that E induced IGF-I gene expression primarily in epithelial cells, whereas P did so in the stroma. Superimposition of E on the P-primed uterus further stimulated IGF-I expression in the stroma. The results of these studies are consistent with an autocrine/paracrine function of uterine IGF-I, and indicate that ovarian steroids regulate the cell-specific and temporal patterns of expression of this gene in the peri-implantation mouse uterus.

Animals↗
PubMed1992

Prevention of estrus in the queen with chlormadinone acetate administered orally.

The possibility of estrus prevention in the queen by the oral administration of chlormadinone acetate was examined. The animals used were 29 mature and 15 immature queens. For 16 mature females, 4-12.5 mg was given daily by mouth for 7 days every 3 months. Ten of the 16 queens given this treatment came into estrus within 4 months of the first treatment. For 28 females including the immature, 2-12.5 mg was given once a week throughout the experiment. This treatment prevented estrous activity for at least 1 year. In the queens in this study, the side effects were not observed excepting an increase in body weight during treatment. Our results showed that oral administration of this drug weekly is safe and reliable for long-range prevention of estrus in queens.

Administration, Oral↗
PubMed1992

[Suppression of interphotoreceptor retinoid-binding protein (IRBP)-induced experimental autoimmune uveitis by pretreatment with anti-idiotypic monoclonal antibody].

In an experimental model of interphotoreceptor retinoid binding protein (IRBP)-induced experimental autoimmune uveitis (EAU) in Lewis rats, EAU onset was suppressed by pretreatment with anti-idiotypic antibody (TRD3). In the pretreated group, the titers of anti-IRBP antibody and idiotypic antibody were lower and the titer of anti-idiotypic antibody was higher than in the control group, and the skin test (DTH) against IRBP was also suppressed. Histopathological findings of the control group showed destruction of retinal outer granular layer, loss of the outer segment and lymphocytic infiltration, although no definite inflammatory signs appeared in the pretreated group. We suppose that the mechanism of idiotypic suppression is via induction of idiotype-specific or idiotype-related suppressor T cells.

Animals↗
PubMed1991

Gene structure of heat shock proteins 61KDa and 12KDa (thermophilic chaperonins) of thermophilic bacterium PS3.

Heat shock proteins 60 (hsp60) and 10 (hsp10) are essential for the formation and restoration of many supramolecular structures. For reconstitution of these structures, we isolated stable hsps of 61kDa and 12kDa, which are similar to hsp60 and hsp10, respectively, from the supernatant fraction of thermophilic bacterium PS3 by ATP-Agarose chromatography. Using synthetic DNA of the deduced sequence, the 1.6kbp double stranded DNA encoding both proteins was obtained by the polymerase chain reaction (PCR). The complete sequence of the resulting reading frames showed high homology to those of the genes encoding GroEL (hsp60) and GroES (hsp10) of E. coli, and hsp60s and hsp10s of several other species. The genes for the 12K and 61K were present in the same operon. 61K was also partially similar to the F1 alpha subunit of thermophilic ATP synthase, which is highly reconstitutable to form the alpha beta complex.

Amino Acid Sequence↗
PubMed1991

Cell-type-specific expression of transforming growth factor-alpha in the mouse uterus during the peri-implantation period.

Immunohistochemistry as well as in situ and Northern blot hybridization were employed to determine temporal and cell-type-specific expression of transforming growth factor-alpha (TGF-alpha) in the mouse uterus during the peri-implantation period. The co-localization of TGF-alpha (by immunohistochemistry) with its mRNA (by in situ hybridization) in the luminal and glandular epithelia on Days 1-4 of pregnancy (Day 1 = vaginal plug) and also in many stromal cells on Days 3 and 4 indicates that these cells are the primary sites of TGF-alpha synthesis during the preimplantation period. The higher levels of TGF-alpha mRNA in total uterine RNA on Day 4, as shown by Northern blotting, is consistent with the recruitment of stromal cells expressing this gene. During the post-implantation period (Days 5-8), the co-localization of the mRNA and protein in the decidua at the implantation sites suggests that the decidualizing stromal cells synthesize TGF-alpha. Although in situ hybridization showed the presence of mRNA in embryos on Days 5-8, immunostaining was noted in the embryo only on Days 5 and 6. These results suggest that uterine and embryonic expression of TGF-alpha during the peri-implantation period could be involved in embryonic development, preparation of the uterus for implantation, and decidualization.

Animals↗
PubMed1991

[Production of anti-33kDa protein monoclonal antibody and organ-specificity of 33kDa protein].

We produced monoclonal antibody TS-SC6 specific for 33kDa protein (33K) from bovine retina and studied the localization of 33K in mammalian retinas (rat, mouse, bovine, guinea pig and human) and pineal gland of rat. An immunohistochemical study showed that TS-SC6 reacted with the outer plexiform layer (OPL), outer nuclear layer (ONL) and rod inner segments (IS) in rat, ONL, IS and IS in mouse and guinea pig. It reacted with ONL and rod outer segments (OS) in bovine, ONL and OS in human. Immunoreactivity was seen in the pineal gland, but there was no immunoreactivity in the central nervous system surrounding the pineal gland. Immunoblot analysis was carried out with soluble fractions prepared from the retina, pineal gland, cerebrum, liver, kidney and intestine of rats. TS-SC6 reacted with 33K of the retina and pineal gland, respectively. However, it did not react with soluble fractions from the other tissues.

Aged↗
PubMed1990

Analysis of the human, bovine and rat 33-kDa proteins and cDNA in retina and pineal gland.

A monoclonal antibody (mAb) was produced against a bovine retinal 33-kDa protein. Several clones of 33-kDa protein were isolated from each library of cDNA from human, bovine and rat retinas and rat pineal gland by mAb screening and by hybridization with cDNA probes. Each of the four cDNA sequences was determined and amino acid (aa) sequences were deduced from the nucleotide sequences. The latter were nearly identical in rat retina and rat pineal gland (99.6%) and were similar in human, bovine and rat retina (more than 87%). Each of these cDNAs had one long ORF and encoded 245 or 246 aa. The deduced aa sequences in rat retina and rat pineal gland were virtually identical and the sequences in human, bovine and rat retina were highly homologous (more than 88%). The predicted Mr for each of these proteins was 28,246 in the human, 28,176 in bovine, 28,143 in rat retina, and 28,129 in rat pineal gland. Each of the sequences has a putative site for phosphorylation by A kinase; we have confirmed that the putative site is Ser73. These results show that the 33-kDa proteins in the retina and pineal gland have the same sequences and the same phosphorylation site and suggest that the functional role of this protein is the same in the retina and pineal gland.

Amino Acid Sequence↗
PubMed1990

Cell type-specific expression of transforming growth factor-beta 1 in the mouse uterus during the periimplantation period.

Immunohistochemistry and in situ and Northern blot hybridization were employed to determine temporal and spatial expression of transforming growth factor-beta 1 (TGF beta 1) in the mouse uterus during the periimplantation period. The polyclonal antisera anti-LC-(1-30) and anti-CC-(1-30), raised against two different preparations of a peptide corresponding to the amino-terminal 30 amino acids of TGF beta 1, were used for histochemical analyses because of their distinct staining patterns. Anti-LC shows intracellular staining, while staining by anti-CC is primarily extracellular. The colocalization of intracellular staining by anti-LC with in situ hybridization of TGF beta 1 mRNA in the luminal and glandular epithelia on days 1-4 of pregnancy (day 1 = vaginal plug) indicates that the epithelial cells are the primary sites of TGF beta 1 synthesis during the preimplantation period. On the other hand, staining of the extracellular matrix of the stroma by anti-CC during this period suggests an active accumulation of TGF-beta 1 that is synthesized in and secreted from the epithelia. While intracellular staining and accumulation of TGF-beta 1 mRNA in the epithelia were clearly evident on days 1-4, the extracellular staining showed temporal fluctuations. The clear extracellular staining of the stroma that was observed on day 1 was absent on day 2; moderate staining was again visualized in the stroma on day 3 and was markedly increased on day 4.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1990

The effect of estrogen and progesterone on the volume-pressure curve of the uterine ampulla in late pregnant rats.

Volume-pressure curves of the uterine ampullae, taken on day 17 of pregnancy from intact pregnant and ovariectomized, vehicle- or estradiol and/or progesterone-treated pregnant rats, during infusion of Ca-free Tyrode's solution containing EGTA at a constant rate were determined, and the effects of the steroids on the Vz value, which was obtained by extrapolating the linear part of the curve to zero pressure, and compliance were evaluated. Both the parameters examined were decreased by ovariectomy. Treatments with estradiol or progesterone alone did not but the steroids combined restored Vz to the level of intact pregnant rats, and partially restored compliance. The significance of the development of Vz by estrogen and progesterone for the maintenance of pregnancy is discussed.

Animals↗
PubMed1985

Effects of proteinase inhibitors on preimplantation embryos in the rat.

Proteinase inhibitors of microbial origin were injected into the uterine horns of mated rats at 14:00 h on Day 5 of pregnancy (spermatozoa in vaginal smear = Day 1), and 5 or 6 h later the embryos were flushed from the horns and examined. Chymostatin and alpha-MAPI, inhibitors of chymotrypsin-like serine proteinase and thiol proteinases, as well as thiolstatin, an inhibitor of thiol proteinases, significantly inhibited embryo growth. The inhibitory activity of alpha-MAPI on embryonic growth was distinctly greater than that of thiolstatin, although the ID50 values of the two inhibitors to papain are similar. Antipain and leupeptin which are inhibitors of trypsin-like and thiol proteinases, and talopeptin, an inhibitor of metal proteinases, significantly interrupted the removal of the zona pellucida from expanding blastocysts. These results suggest that (1) a chymotrypsin-like proteinase seems to be important to the growth of the embryo, (2) a thiol proteinase may participate in embryonic growth, and (3) a trypsin-like proteinase and a metal proteinase are likely to participate in zonalysis.

Animals↗
PubMed1984

[The effect of androgen on the maintenance of pregnancy in progesterone-treated ovariectomized rats].

Pregnant rats ovariectomized on day 14 of pregnancy (Sperm present = day 1) were daily treated with 4 mg progesterone (P) plus androgens, and the effects of the androgens on fetal survival and intrauterine pressure were examined on day 20 of pregnancy. In the rats treated with P only, the percentage of live normal fetuses was only 40.4% of the total fetuses, 39.4% were injured fetuses having hematoma on their extremities, and 20.2% were absorbing. Intrauterine pressure was about three times higher than that in the sham-operated controls. Treatment with androgen as well significantly increased the percentage of live normal fetuses and decreased intrauterine pressure, as estrogen did. A significant negative correlation was observed between the percentage of live normal fetuses and intrauterine pressure. Androstenediol (A-diol) was most effective and its daily dose of 2 microM maintained normal pregnancy. A-diol and dehydroepiandrosterone (DHA) were more effective than androstenedione (A-dione) and testosterone (T), suggesting that the effect of the former two androgens is not by conversion to estrogen, but by their direct action on the uterine wall. 5 alpha-dihydrotestosterone, a non-aromatizable androgen, also exhibited moderate estrogenic action.

Androgens↗
PubMed1984

Inhibition of in-vitro fertilization of hamster oocytes by a thiol proteinase inhibitor.

Amongst the proteinase inhibitors tested, thiolstatin, a specific inhibitor for the thiol proteinases, leupeptin and antipain, both specific inhibitors of serine- and thiol-proteinases, strongly reduced fertilization of hamster oocytes in vitro. These results suggest the possible involvement of thiol proteinase(s), as well as acrosin, in the fertilization process. A possible role for thiol proteinase in sperm adhesion to the zona pellucida is proposed.

Animals↗
PubMed1984

The effect of estrogen on passive length-tension relationship in the uterus of ovariectomized progesterone-treated pregnant rats.

Passive length-tension curves of uterine strips obtained on day 20 of pregnancy from ovariectomized, progesterone-treated (OVX-P), progesterone + estrogen-treated (OVX-PE), and intact pregnant rats (control) were determined, and the effects of ovarian hormones on the length of the collagenous framework (LZ), tension at in vivo length (TV), and distensibility (D) of the strips, which were obtained from the curves, were evaluated. LZ of circular strips, but not of longitudinal strips, of OVX-P rats was smaller than the in situ length (20 mm) of the strips, whereas the value of control rats was greater than 20 mm; TV of these strips was 4.5-fold of controls. Treatment with estrogen restored both LZ and TV to the control levels. D of both longitudinal and circular strips of OVX-P rats was smaller than the controls, estrogen increased D to longitudinal direction predominantly. Examination in Ca-free Tyrode's had no influence on any of LZ, TV or D. Results suggest that estrogen with progesterone develops the collagenous framework of the connective tissue to radial direction and distensibility of the uterine wall mainly to longitudinal direction, and thereby maintains the tension of the uterine wall to accommodate the product of conception in the rat.

Animals↗
PubMed1983

The effect of ovarian hormones on stress relaxation in the uterus of the pregnant rat.

Tissue strip taken from rat uterine ampulla on day 20 of pregnancy was elongated at a contrast rate in Tyrode's solution at 37 degrees C to the length of a specified peak stress, and the stress decline was recorded at this length. The stress decline in uterine strips of ovariectomized progesterone-treated rats was significantly slower than those in intact pregnant rats at any different peak stress and elongation rate examined. Treatment with estradiol as well restored the physical properties to the control levels. Changing the medium to Ca-free Tyrode's solution had no effect on stress relaxation. Results suggest that estrogen with progesterone improves stress relaxation in the rat uterus during late pregnancy by changing the state of the noncontractile elements in the tissue.

Animals↗