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H Tedeschi

Publications and source records attributed to H Tedeschi.

At least 19 recordsLinked to original sources
PubMed1992

The effect of antimycin A on mouse liver inner mitochondrial membrane channel activity.

In a patch-clamp study, we found antimycin A in low (1-2) microM concentrations decreased the open probability of the multiple conductance channel activity and the approximately 110 picosiemens channel of the inner mitochondrial membrane (for a review of mitochondrial channels see Kinnally, K. W., Antonenko, Yu. N., and Zorov, D. B. (1992) J. Bioenerg. Biomembr. 24, 99-110). Higher antimycin A concentrations (e.g. 10 microM) facilitated multiple conductance channel opening. These effects were reversible, and the binding site(s) are probably distinct from those responsible for the inhibition of the electron transport chain, since the latter are virtually irreversible. A model with two closed and two open states is presented for the approximately 110-picosiemens activity.

Animals↗
PubMed1992

Multiple conductance levels in rat heart inner mitochondrial membranes studied by patch clamping.

The behavior of the mitochondrial inner membrane multiple conductance channel (MCC) which has a peak conductance of 1-1.5 nS has been examined in rat heart mitochondria. MCC can display several unique characteristics: (a) prolonged open and closed times on the order of seconds to minutes, (b) a voltage dependence in which MCC opens (negative potential) or closes (positive potential) generally in steps, (c) a response to inhibitors such as amiodarone in steps corresponding at least approximately to those in (b), (d) a 'free-running mode' in which the current level rapidly fluctuates between a minimum of nine conductance levels but with a preferred occupation of the 0.5-0.7 nS levels, and (e) very large transitions (1-1.5 nS) resolved at 4 kHz bandwidth as single events with variable mean open time.

Animals↗
PubMed1992

Voltage activation of heart inner mitochondrial membrane channels.

The patch clamp records obtained from mitoplast membranes prepared in the presence of a calcium chelator generally lack channel activity. However, multiconductance channel (MCC) activity can be induced by membrane potentials above +/- 60 mV [Kinnally et al., Biochem. Biophys. Res. Commun. 176, 1183-1188 (1991)]. Once activated, the MCC activity persists at all voltages. The present report characterizes the activation by voltage of multiconductance channels of rat heart inner mitochondrial membranes using patch-clamping. In some membrane patches, the size of single current transitions progressively increases with time upon application of voltage. The inhibitor cyclosporin has also been found to decrease channel conductance in steps. The results suggest that voltage-induced effects which are inhibited by cyclosporin A are likely to involve either an increase in effective pore diameter or the assembly of low-conductance units. In activated patches, we have found at high membrane potentials (e.g., 130 mV) changes in conductance as high as 5 nS occurring in large steps (up to 2.7 nS). These were generally preceded by a smaller transition. Similar results were obtained less frequently at lower voltages. These results can be explained on the assumption that once assembled the channels may act in unison.

Animals↗
PubMed1992

Modified supraorbital craniotomy: technical note.

The authors present a surgical approach that incorporates the frontal sinus and extends a supraorbital craniotomy to include the lateral orbital rim and zygoma. The craniotomy provides wide exposure of the anterior fossa, orbit, ipsilateral middle fossa, and cavernous sinus. The procedure can be performed easily, and the bone flaps can be secured rapidly back into the anatomical position at the time of closure. This modified supraorbital craniotomy is ideal for large benign lesions originating along the sphenoid wing or orbit that expand into the anterior fossa.

Craniotomy↗
PubMed1992

Microsurgical anatomy of acoustic neuroma.

Because acoustic neuromas most frequently arise in the posteriorly placed vestibular nerves, they usually displace the facial and cochlear nerves anteriorly (Figs. 11, 12, and 13). The facial nerve is stretched around the anterior half of the tumor capsule. Variability in the direction of growth of the tumor arising from the vestibular nerves may result in the facial nerve being displaced, not only directly anteriorly, but also anterior-superiorly or anterior-inferiorly. The nerve is infrequently found on the posterior surface of the tumor. Because the facial nerve always enters the facial canal at the anterior-superior quadrant of the lateral margin of the meatus, it is usually easiest to locate it here, rather than at a more medial location where the degree of displacement of the nerve is more variable. The cochlear nerve also lies anterior to the vestibular nerve and is most frequently stretched around the anterior half of the tumor. The strokes of the fine dissecting instruments used in removing the tumor should be directed along the vestibulocochlear nerve from medial to lateral rather than from lateral to medial because traction medially may tear the tiny filaments of the cochlear nerve at the site where these filaments penetrate the lateral end of the meatus to enter the cochlea. The landmarks that are helpful in identifying the facial and vestibulocochlear nerves at the brain stem on the medial side of the tumor have been reviewed. These nerves, although distorted by tumor, can usually be identified on the brain stem side of the tumor at the lateral end of the pontomedullary sulcus, just rostral to the glossopharyngeal nerve and just anterior-superior to the foramen of Luschka, flocculus, and choroid plexus protruding from the foramen of Luschka. After the facial and vestibulocochlear nerves are identified on the medial and lateral sides of the tumor, the final remnants of the tumor are separated from the intervening segment of the nerves. In the three approaches to the meatus and cerebellopontine angle--retrosigmoid, translabyrinthine, and middle fossa--a communication may be established between the subarachnoid space and the mastoid air cells that requires careful closure to prevent a cerebrospinal fluid leak.

Brain Stem↗
PubMed1991

Selective effect of inhibitors on inner mitochondrial membrane channels.

The effect of amphiphilic cationic drugs on the channel activity of the mitochondrial inner membrane was examined with patch-clamp techniques. The therapeutic drugs amiodarone, propranolol and quinine reduced the probability of being open for the multiconductance channel (MCC) activity (levels from 30 pS to over 1 nS). While amiodarone decreased the probability of being open for the voltage dependent approximately 100 pS channel, it increased the conductance 42 +/- 20% (mean +/- SD, n = 6) with no significant change in mean open time. Similar results were obtained with propranolol. These data indicate that the approximately 100 pS channel is distinct from MCC activity.

Amiodarone↗
PubMed1991

Identification of anion and cation pathways in the inner mitochondrial membrane by patch clamping of mouse liver mitoplasts.

Alkalinization of the matrix side of the mitochondrial inner membrane by pH shifts from 6.8 to 8.3 caused a reversible increase in current of 3.2 +/- 0.2 pA (mean +/- SE, n = 21) at +/- 40 mV measured using patch-clamp techniques. The current increase was reversed in a graded fashion by the addition of Mg2+ as well as a reduction in pH. Detection of single-channel events was done at 0.5, 1 and 2 M KCl. The single-channel amplitude in 0.15 M KCl corresponds to approximately 15 pS. Reversal potentials derived from whole patch currents indicated that the inner mitochondrial membrane was primarily cation selective at pH 6.8 with a PK/PCl = 32 (n = 6). Treatment with alkaline pH (8.3) increased the current and anion permeability (PK/PCl = 16, n = 6). The membrane becomes completely cation selective when low concentrations (12 microM) of the drug propranolol are added. The amphiphilic drugs amiodarone (4 microM), propranolol (70 microM) and quinine (0.6 mM) blocked almost all of the current. The pH-dependent current was also inhibited by tributyltin. These results are consistent with the presence of two pathways in the inner mitochondrial membrane. One is cation selective and generally open and the other is anion selective and induced by alkaline pH. The alkaline pH-activated channel likely corresponds to the inner membrane anion channel postulated by others from suspension studies.

Amiodarone↗
PubMed1991

Anatomic examination of a case of open trigeminal nucleotomy (nucleus caudalis dorsal root entry zone lesions) for facial pain.

Nucleus caudalis dorsal root entry zone lesions (open trigeminal nucleotomy) are a surgical procedure which can achieve pain control without major complications in the difficult clinical setting of deafferentation-type facial pain. Two patients are reported, who had relief of pain, but also experienced neurological complications. One patient succumbed to pulmonary complications, which provided the opportunity for anatomic analysis of the lesioned area, which is discussed in detail. Potential modifications of the surgical technique are suggested.

Afferent Pathways↗
PubMed1978

Metabolic effects of some electrofluorimetric dyes.

The effect of five electrofluorimetric dyes on mitochondrial metabolism was examined to determine their suitability for mitochondrial studies and other biological uses. The dyes merocyanine 540, 8-anilino-1-naphthalene sulfonic acid and bis(1,3-dibutyl barbituric acid-(5))-pentamethane oxonol were found to be inhibitors of the respiratory chain. However, the first two exerted their effect only at high concentrations. 3.3'-Dihexyl-2,2'-oxacarbocyanine was found to act as an uncoupler. 3,3'-Dipropyl-thiocarbocyanine inhibited beta-hydroxybutyrate respiration while dissociating succinate supported respiration from the phosphorylation of ADP. Merocyanine 540 and 8-anilino-1-naphthalene sulfonic acid may be the best suited for studies of membrane potentials in mitochondria since their effect on metabolism is negligible

Animals↗
PubMed1978

Use of dyes to estimate the electrical potential of the mitochondrial membrane.

A number of cationic or anionic fluorescent dyes were investigated as possible monitors of the membrane potential of rat liver mitochondria, and giant mitochondria isolated from the liver of mice maintained on a diet containing cuprizone. The fluorescence of four dyes (8-anilino-1-naphthalenesulfonic acid, merocyanine 540, 3,3'-dipropyl-thiocarbocyanine, and bis[1,3-dibutylbarbituric acid-(5)]-pentamethine oxonol) was found to respond appropriately to changes in an apparent K+ diffusion potential. Generally, valinomycin-induced K+ diffusion potentials as calculated using the Nernst equation were used to calibrate the dependence of the fluorescence on the membrane potential. The appropriateness of this approach was verified for two dyes using microelectrodes in giant mitochondria. The apparent membrane potential change induced by the addition of succinate was variable but was very low and generally less than 60 mV in magnitude. The results are consistent with the notion that a large membrane potential is not established upon the initiation of metabolism and that the membrane potential does not play a significant role in the observed ADP phosphorylation.

Animals↗
PubMed1978

Membrane potentials and resistances of giant mitochondria. Metabolic dependence and the effects of valinomycin.

The membrane potentials and resistances of giant mitochondria from mice fed cuprizone have been studied. They were found to correspond approx. 10-20 mV, positive inside, and 2 M omega, respectively. These properties were found to be independent of the metabolic state. The microelectrodes were in the inner mitochondrial space since (a) the potentials in the presence of valinomycin depended on the K+ concentration of the medium and magnitude of the K+ diffusion potentials was consistent with the presence of a high internal concentration of K+, (b) almost identical results were obtained with mitochondria from which the external membrane had been removed and the cristae were evaginated, and (c) punch-through experiments, in which the microelectrodes were advanced until they emerged through the other side of the mitochondria, showed an identical membrane potential both in the presence and in the absence of valinomycin. The potentials were stable under a variety of conditions and showed no sign of decay of membrane leakiness. Detailed evidence that the impaled mitochondria are metabolically viable will be presented in a separate publication.

Animals↗
PubMed1978

Assays of the metabolic viability of single giant mitochondria. Experiments with intact and impaled mitochondria.

Single giant mitochondria isolated from mice fed cuprizone were assayed for their metabolic viability. Two tests were devised. One test optically detected the accumulation of calcium phosphate within the mitochondria under massive loading conditions (including the presence of succinate and ATP). The accumulation corresponds to a test of energy coupling from either electron transport or the hydrolysis of ATP since it is blocked by either antimycin A or oligomycin. The other assay tested for the production of ATP from ADP and Pi, using myofibrils. Myofibrils prepared from glycerinated rabbit psoas muscle contract only in the presence of ATP and not in the presence of ADP. Myofibrillar contraction is unaffected by the presence of antimycin A or oligomycin. However, myofibrils in the presence of mitochondria that are phosphorylating ADP to ATP do contract. This contraction is blocked by antimycin A and/or oligomycin. Hence, the ATP which causes myofibrillar contraction is produced by oxidative phosphorylation. At low mitochondrial concentration, only the myofibrils in close proximity with mitochondria contract in the presence of ADP. Therefore the assay can be used to test the viability of individual mitochondria. Individual giant mitochondria were found to be viable, using both of these assays. Comparable results were obtained in mitochondria impaled with microelectrodes. The potentials and resistances were unaffected by concomitant calcium phosphate accumulation or oxidative phosphorylation.

Adenosine Diphosphate↗
PubMed1977

Membrane potential of mitochondrial measured with microelectrodes.

The membrane potentials of giant mitochondria from cuprizone-fed mice were found to be independent of metabolic state. Experiments are described in which the presence of the microelectrodes in the inner mitochondrial space, and the metabolic viability of the impaled mitochonidra, are validated.

Animals↗
PubMed1976

Osmotic behavior and permeability of osmotically lysed mitochondria.

Experiments were carried out with water-treated isolated rat liver mitochondria (mitochondria ghosts) previously studied by Caplan and Greenawalt (Caplan, A.I., and J.W. Greenawalt. 1966. J. Cell Biol. 31:455-472) and Vasington and Greenawalt (Vasington, F., and J. Greenawalt. 1968. J. Cell Biol. 39:661-675). The ghosts have permeability properties and osmotic behavior comparable to those of isolated mitochondria. Although they have lost most of their internal contents, they must have resealed. Four properties were found which have not been previously described in systems derived from biological membranes: (a) an osmotic behavior in the virtual absence of internal components. (b) a self-arranging property in the formation of invaginations corresponding in morphology to the cristae. The results suggest that the assembly of the molecular components of the inner membrane is sufficient to specify the morphology. Hence the surface area to volume ratio of the vesicles may specify the presence or absence of cristae-like folds. (c) an increase in the permeability of the membranes to sucrose in the presence of iso-osmotic concentrations of sucrose. (d) an independence of the light transmitted by suspensions of the vesicles from the refractive index of the external medium. This observations run counter to the general previous experience with either mitochondria or liposomes.

Animals↗