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Biomedical subjects

H Thompson

Publications and source records attributed to H Thompson.

At least 145 records · Page 8Linked to original sources
PubMed1988

The immunological relationship between canine herpesvirus and four other herpesviruses.

Canine herpesvirus (CHV) was compared with four other herpesviruses by several serological techniques. Cross-neutralization was demonstrated between CHV and herpes simplex virus types 1 and 2 and pseudorabies virus. Non-neutralizing cross-reactions were found with these viruses and also with equine abortion virus and bovine mammillitis virus. The data suggest that CHV is immunologically more closely related to herpes simplex virus than to the other viruses used in this study.

Antibodies, Viral↗
PubMed1988

Use of histological examination to assess ultrastructure of liver in patients with long standing jejuno-ileal bypass for morbid obesity.

To assess the pattern and severity of liver disease in patients who had undergone jejuno-ileal bypass for the treatment of morbid obesity, 23 patients were biopsied, all of whom had had intact bypasses for more than 10 years. These were examined by light and electron microscopy. Previous biopsy specimens from each patient, including specimens taken before bypass, were reviewed. Similar biopsy specimens were obtained from six obese patients undergoing gastroplasty. There was no evidence of cirrhosis, and mild or moderate degrees of fibrosis were found in only a few patients. Steatosis tended to persist after bypass, albeit to a lesser degree. Giant mitochondria and intramitochondrial filamentous inclusions were present in four of six specimens taken before gastroplasty and in 15 of 23 specimens taken after jejuno-ileal bypass, being especially numerous in those specimens showing little or no steatosis. It is suggested that such features reflect ultrastructural evidence of adaptation to an abnormal metabolic environment both in the morbidly obese and even many years after jejuno-ileal bypass. Their prognostic importance is unclear.

Adult↗
PubMed1988

Early onset high grade dysplasia in ulcerative colitis.

We report a patient with ulcerative colitis who underwent colectomy when aged 25 years for multifocal high grade dysplasia which had developed within 2 years of diagnosis, and only 5 1/2 years after his first symptoms. The implications of this case for the management of ulcerative colitis are discussed.

Adult↗
PubMed1988

Studies on canine parvovirus infection: preparation of challenge virus.

Two techniques, adsorption on to hydroxylapatite and density gradient centrifugation, were investigated as prospective methods for the large scale purification of canine parvovirus from faecal suspensions. Adsorption with hydroxylapatite successfully removed virus from faecal material. However, the resultant virus was contaminated and some virus was left behind in the faecal suspension. Repeated adsorption with hydroxylapatite appeared to result in some damage to the virus particles. In contrast, density gradient centrifugation provided a simple, economical method of purification which yielded uncontaminated, infectious virus. The final method, using both isopyknic and rate zonal centrifugation is described.

Animals↗
PubMed1988

Multiple bidirectional alterations of phenotype and changes in proliferative potential during the in vitro and in vivo passage of clonal mast cell populations derived from mouse peritoneal mast cells.

Mouse peritoneal mast cells (PMC) express a connective tissue-type mast cell (CTMC) phenotype, including reactivity with the heparin-binding fluorescent dye berberine sulfate and incorporation of [35S] sulfate predominantly into heparin proteoglycans. When PMC purified to greater than 99% purity were cultured in methylcellulose with IL-3 and IL-4, approximately 25% of the PMC formed colonies, all of which contained both berberine sulfate-positive and berberine sulfate-negative mast cells. When these mast cells were transferred to suspension culture, they generated populations that were 100% berberine sulfate-negative, a characteristic similar to that of mucosal mast cells (MMC), and that synthesized predominantly chondroitin sulfate [35S] proteoglycans. When "MMC-like" cultured mast cells derived from WBB6F1-+/+ PMC were injected into the peritoneal cavities of mast cell-deficient WBB6F1-W/Wv mice, the adoptively transferred mast cell population became 100% berberine sulfate-positive. In methylcellulose culture, these "second generation PMC" formed clonal colonies containing both berberine sulfate-positive and berberine sulfate-negative cells, but exhibited significantly less proliferative ability than did normal +/+ PMC. Thus, clonal mast cell populations initially derived from single PMC exhibited multiple and bidirectional alterations between CTMC-like and MMC-like phenotypes. However, this process was associated with a progressive diminution of the mast cells' proliferative ability.

Animals↗
PubMed1987

Plateletapheresis using single vein access: a comparison of Haemonetics V-50 and Fenwal CS-3000 blood cell separators.

Donors were randomly selected for plateletapheresis using either the Haemonetics V-50 surge procedure or the Fenwal CS-3000 with special modifications. Six procedures using the V-50 were performed with 6 collection cycles. Platelet yields averaged 3.9 X 10(11) with an average white cell contamination of 3.8 X 10(8). Ten procedures were performed with 8 collection cycles. Platelet yields averaged 4.5 X 10(11) with an average white cell contamination of 1.2 X 10(8). CS-3000 procedures were performed using either 4,000 or 4,800 ml whole blood processed as the endpoint. Concentrates obtained using 4,000 ml (n = 10) as the endpoint contained 3.9 X 10(11) platelets with a white cell contamination of 9.2 X 10(8). A 4,800 ml (n = 5) endpoint produced concentrates with a yield of 4.5 X 10(11) and white cell contamination of 10.4 X 10(8).

Blood Component Removal↗
PubMed1986

Crohn's disease involving the penis.

Perianal complications of Crohn's disease occur in 25-70% of patients but perineal and genital lesions are rare. Treatment is controversial and there is a risk of recurrent or persistent disease. We report two cases of Crohn's disease involving the penis, one with multiple scrotal urinary fistulae, partial destruction of the proximal urethra, and ulceration of the penile shaft; the other with metastatic cutaneous ulceration of the penile shaft. The second case is particularly unusual in that the patient presented at the VD clinic as a case of syphilis.

Adolescent↗
PubMed1986

Mammographic identification and biopsy of occult breast cancer.

During a 9 year period we excised 70 suspicious non-palpable breast lesions detected by mammography. We found the most effective method of preoperative localisation to be insertion of a self retaining wire, and this procedure was facilitated by a Perspex grid incorporated in the mammography equipment. Thirty biopsies (43%) were found to be malignant, of which 14 contained invasive and 16 in situ carcinoma. The presence of malignancy could not be predicted from the mammographic appearance, although in situ cancer was always associated with microcalcification.

Adult↗