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Biomedical subjects

H Tsunooka

Publications and source records attributed to H Tsunooka.

16 recordsLinked to original sources

[Two resected cases of malignant fibrous histiocytoma originated from the thoracic soft tissues].

Two resected cases, a 56-year-old man and a 49-year-old man, of malignant fibrous histiocytoma originated from the soft tissues of chest wall were reported. In both cases, MFH was histologically diagnosed prior to our radical operation. One patient received an incisional biopsy and the other was a recurrent case after excisional resection in other hospital. We resected the whole chest wall together with the tumor sufficiently apart from the tumor margin. In one patient the defect of chest wall was reconstructed with the material made of bone cement which sandwiched between 2 sheets of marlex heavy mesh and covered by latissimus dorsi muscle flap on it. The other was reconstructed with single sheet of marlex heavy mesh and covered by rectus abdominis muscle flap. Recurrence was not seen in either of two patients, in spite of the general acknowledgment that MFH recurs frequently and locally. We considered that the extended wide resection including the whole chest wall should be performed, and that marlex heavy mesh and the pedunculated musculo-cutaneous flap was useful for the reconstruction of chest wall defect.

Histiocytoma, Benign Fibrous↗

[Imaged thoracoscopic surgery for bronchogenic cyst in posterior mediastinum--a case report].

A 44-year-old female with bronchogenic cyst in the posterior mediastinum was treated successfully by employing imaged thoracoscopic surgical methods in the same way as laparoscopic cholecystectomy. The patient was placed in the left lateral decubitus position. A first 3.5 cm skin incision was made in the seventh intercostal space on the mid-axillary line. A 10 mm, 0-degree Olympus wide angle telescope was inserted for observation into the thoracic cavity. And three more small skin incisions were done. A grasping forceps, a padron retractor and an electrode with J-hook tip were introduced through each incision. The cyst was completely dissected almost with the J-hook electrode. She was discharged on the 10th-postoperative day without complication and with minimal pain. We suggest that an indication of the imaged endoscopic surgery could be established for some benign mediastinal tumors.

Adult↗

[A case report of solitary bone cyst of rib detected from pathological fracture].

A case of solitary bone cyst of rib was reported. The patient was 28-year-old man who was admitted to our hospital complaining of chest pain caused by pathological fracture of the left 10th rib. Chest x-ray film revealed a solitary cystic lesion of the 10th rib. The tumor was removed by extrapleural rib partial resection and the histological examination confirmed the diagnosis of solitary bone cyst.

Adult↗

VMA mass screening program of neuroblastoma for infants in Nagoya City, Japan.

In Nagoya City, Japan, VMA mass screening for detection of neuroblastoma has been performed since 1977. Filter paper strips wetted with urine are mailed to a central laboratory. Positive tests are followed up by more definitive testing. Of the 20,053 urine samples of 6-month-old infants tested, January 1977 to March 1983, five asymptomatic infants with neuroblastoma have been discovered. All have been treated and are alive as of January 1984.

Child, Preschool↗

Characterization and partial purification of neuroblastoma growth inhibitory factor from the culture medium of glioblasts.

Neuroblastoma growth inhibitory factor (NGIF) exists in the conditioned medium of normal rat glioblasts. When neuroblastoma cells (Neuro2a, NS-20Y, and N1E-115) were cultured in the presence of the factor, the cell growth rates and DNA synthesis were markedly inhibited and the morphological differentiation including neural process formation was induced. However, the factor neither altered the growth rate nor the morphology of non-neuronal cells such as glial cell lines (C6 and 354A) or fibroblast (3T3). The molecular weight of the factor was estimated to be 75,000 Mr by gel filtration with Bio-Gel P-200, and the isoelectric point was 5.8. The factor was devoid of esteropeptidase activity, and susceptible to protease and thermal treatment. The growth inhibitory action of the factor was unrelated to the intracellular contents of cyclic AMP and GMP. The ability of NGIF to suppress preferentially the neural growth suggests its regulatory role in normal brain development.

Animals↗

Serum acute phase reactants in pediatric patients; especially in neonates.

We studied serum acute phase reactant (APR) levels in 45 pediatric surgical patients. Alpha 1-acid glycoprotein (alpha 1-AG) showed a peak value on day 3 postoperatively (P.O.) and alpha 1-antitrypsin (alpha 1-AT) showed a high value on days 3-5 P.O. These glycoproteins returned to normal levels by day 21 P.O., but the level of haptoglobin (Hp) remained high until day 21 P.O. The postoperative changes of alpha 1-AG and alpha 1-AT correlated with the process of recovery from inflammatory conditions, but C-reactive protein (CRP) reached a peak on days 1-2 P.O. and returned to normal limits by day 14 P.O. In patients with infection, CRP returned to the normal level rapidly before recovery from infection. Of the 3 glycoproteins, alpha 1-AG seemed to be a valuable indicator of the pathological conditions. Postoperative changes in APR levels should be useful for early detection of postoperative complications. Persistent ileus led to an increase in APR levels, as a consequence of an inflammatory reaction due to breakdown of the intestinal mucosal barrier.

Abdomen↗

The induction of glial proliferation by an astrocytoma-derived growth factor resembling glia maturation factor.

Glia maturation factor (GMF)-like activity which induces DNA synthesis and morphological differentiation of density-inhibited glioblasts was detected in various glial tumor cells. A polypeptide from C6 cells (rat astrocytoma) which has a molecular weight range of 40,000-50,000 showed the highest activity. This factor also induced DNA synthesis in glioma cells (354A and LRM55) and fibroblast (Swiss 3T3). The activity was susceptible to heat treatment at 70 degrees C for 5 min, or to proteases such as trypsin, chymotrypsin, papain, and subtilisin, but it was devoid of esteropeptidase activity. The isoelectric point was found to be 5.3. Subcellular fractionation localized the activity in cytosomal and microsomal fractions. These properties closely resemble those of GMF from pig and bovine brain.

Animals↗

Inhibition of growth of mouse neuroblastoma cels by protein factor derived from rat glioblasts.

Growth inhibitory factor for mouse neuroblastoma cells was detected in the culture medium of fetal rat glioblasts, and was partially purified and characterized. The factor had an apparent molecular weight of 75,000 and an isoelectric point of 5.8, and showed no esterase activity. It possessed the activity to promote morphological differentiation including the formation of neural processes and the inhibition of cell division when tested on mouse neuroblastoma cell lines (Neuro 2a, NS-20Y, and NIE-115). The activity was susceptible to protease digestion and heat treatment. The serum over 25% cancelled the inhibitory activity of this factor. The factor failed to increase the intracellular contents of cAMP and cGMP. It showed no effect on either morphological differentiation or proliferation of glial cell lines, suggesting that under physiological conditions the factor acts specifically on neuronal cells.

Animals↗

Glial cell growth-promoting factor in astrocytoma (C6) cell extracts.

The presence of glia maturation factor (GMF)-like activity was demonstrated in rat astrocytoma cells (C6 cells). The extracts of C6 cells enhanced the DNA synthesis of cultured glioblasts 3-fold at the maximum, inducing such morphological changes as extrusion of processes. C6 cells also showed a proliferative response to the extracts, though the responsiveness in terms of effective concentration of C6 extracts was about a half of the glioblast responsiveness. The extracts lowered growth rate of neuroblastoma cells and especially decreased their DNA synthesis without a morphological differentiation.

Animals↗