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Biomedical subjects

H Uda

Publications and source records attributed to H Uda.

At least 37 records · Page 2Linked to original sources
PubMed1994

Human papillomavirus not found in squamous and large cell lung carcinomas by polymerase chain reaction.

BACKGROUND: Human papillomavirus (HPV) has been identified not only in anogenital carcinomas, but also in malignancies of other organs, including bronchogenic carcinomas. Previous studies reporting detection of these viruses in lung cancers used mainly in situ hybridization. The authors applied the polymerase chain reaction (PCR) for investigating the occurrence of HPV in bronchial neoplasms. METHODS: Formalin fixed, paraffin embedded tissues of 40 squamous and 7 large cell lung cancers were examined. PCR was done with consensus primers, capable of detecting HPV 6, 11, 16, 18, 31, 33, 52b, and 58. RESULTS: None of the 47 samples contained any of the examined HPV types. CONCLUSIONS: Because the squamous and large cell cancer cases were found not to be associated with HPV infection, this study does not support the potential role of these viruses in the development of lung cancers.

Base Sequence↗
PubMed1994

Pigmented squamous cell carcinoma of the alveolar ridge in the oral mucosa.

The clinical and morphologic features of a pigmented squamous cell carcinoma of the alveolar ridge in an 81-year-old Japanese woman are reported. The tumor was typical, well-differentiated squamous cell carcinoma but had many melanin-containing cells within it. Electron microscopy showed melanosomes in macrophages, melanocytes, and neoplastic squamous cells. Those in the neoplastic squamous cells seemed to have been excreted from the cytoplasmic processes of melanocytes.

Aged↗
PubMed1994

Rapid techniques for DNA extraction from routinely processed archival tissue for use in PCR.

AIMS: To evaluate the ability of four rapid DNA extraction methods to provide DNA for the polymerase chain reaction (PCR) from routinely fixed, paraffin wax embedded archival tissues. METHODS: Eighteen blocks of various tissues, 18 blocks of cervical cancer specimens, and nine blocks of B cell lymphomas were investigated. Both normal and biopsy specimen sized tissues were studied. DNA was extracted using four methods: boiling for 20 minutes in distilled water; boiling for 20 minutes in 5% Chelex-100 resin solution; 3-hour proteinase K digestion; and 3-hour proteinase K digestion, followed by boiling in 5% Chelex-100. Different exons of the p53 gene, human papillomavirus type 16 (HPV 16) sequence, and immunoglobulin heavy chain (IgH) gene rearrangement were amplified from the extracts. RESULTS: The Chelex boiling, proteinase K digestion, and proteinase K digestion-Chelex boiling methods produced DNA suitable for amplification in all of the 45 samples. Boiling in water yielded insufficient template for the PCR in three of the 45 cases (7%), and in six of 42 positive cases (14%) much fainter bands were observed, mostly when the processed material was either biopsy specimen sized or a B cell lymphoma sample. Fragments of the p53 gene were successfully amplified up to 408 base pairs in water boiled extracts, up to 647 in Chelex boiled preparates, and up to 984 in proteinase K digested and proteinase K digested-Chelex boiled samples, although with decreased sensitivity in the last case. All of the templates were reusable after 3 months of storage at -20 degrees C. CONCLUSIONS: Chelex boiling, proteinase K digestion, and proteinase K digestion followed by Chelex boiling produce suitable templates for the PCR from a large variety of paraffin wax embedded tissues. As the simple 20 minute boiling method in 5% Chelex-100 solution requires minimal manipulation and time, it could be useful, especially in the routine processing of large amounts of material.

Base Sequence↗
PubMed1994

[Immunohistological study of dendritic cells and macrophages in sino-nasal lesions--distributions in metaplastic squamous epithelium and squamous cell carcinoma].

Dendritic cells, macrophages and lymphocytes infiltrating the epithelia of sino-nasal lesions were investigated immunohistochemically using antibody S-100 protein for dendritic cells, Ki-M1P for macrophages, MT1 for T-cells and L26 for B-cells, respectively. Seventy-five specimens of sino-nasal mucosae involving 51 cases of metaplastic squamous epithelium (MSE), 12 of inverted papilloma, 1 of atypical hyperplasia and 11 of squamous cell carcinoma (SCC) were studied. Dendritic cells were observed in 12 cases of MSE (23.5%), in 4 of inverted papilloma (33.3%), and in 6 of SCC (54.5%). More dendritic cells were found infiltrating MSE of the infective and fibrotic types than of the mild and edematous types, histopathologically. Furthermore, infiltration correlated with the degree of squamous differentiation in MSE, but not in SCC. The number of macrophages was significantly smaller in MSE than in columnar ciliated epithelium, while that in SCC was significantly greater than that in MSE. With dendritic cell infiltration macrophages in MSE as well as SCC and T-cells in and around the carcinoma were often increased. These findings suggest that dendritic cells may be attracted to maturative squamous epithelium, and may play an important role in the immunologic defence mechanisms of MSE. Dendritic cells, in contrast, may play a role in the cellular immune response of T-cells against the carcinoma.

Adolescent↗
PubMed1993

Human T lymphotropic virus type I associated myelopathy with pulmonary and cutaneous lesions.

A necropsy case of human T lymphotropic virus I (HTLV-I) associated myelopathy (HAM) in a 64 year old man with serological and genetical confirmation of HTLV-I infection is reported. The spinal cord, lung, and skin were mainly affected. Severe degeneration had occurred in the spinal cord, not only in the lateral columns but also in the anterior and posterior columns. The degenerate lesions showed proliferation of capillaries, loss of myelin and axon, and perivascular and parenchymal infiltration with T lymphocytes and foamy macrophages in the white matter. T lymphocytes had infiltrated the lung and there was vascular proliferation in the peribronchus. OPD4 positive cells predominated in the lung. The patient also had erythrodermia where dense and bandlike HTLV-I infected lymphoid cell infiltration was observed, with mild atypia and epidermotropism. HTLV-I may cause multiorganic inflammatory disorders, although the definitive role of HTLV-I in the pathogenesis is still unknown.

Bronchial Diseases↗
PubMed1993

[The effect of Habu toxoid--from the view point of public health center].

In order to clarify the effect of Habu Toxoid, serum sampling was performed with from July 1990 to February 1991, on 503 vaccinated subjects living in Amami Islands by staff of Naze health center. Sera were analyzed by Enzyme Linked Immunosorbent Assay (ELISA) for levels of serum antitoxin to venom, both anti-Hemorrhagic Factor 1 (anti-HR1) and anti-Hemorrhagic Factor 2 (anti-HR2). Information on vaccinated subjects-age, sex, occupation, vaccination date, frequency, period and interval of vaccinations, years after final vaccination and past history of Habu bites-was obtained and analyzed with relation to serum antitoxin levels. The following results were obtained: 1) Serum antitoxin levels, both anti-HR1 and anti-HR2, of the Habu bitten group (N = 47) were significantly higher than that of the unbitten group (N = 456). This finding suggests that crude Habu venom injected by bites elevated the levels. Regardless of past history of Habu bites, levels of antibody to HR2 was significantly lower than that to HR1. 2) Among the unbitten group, detection of antitoxin was related to subjects' attributes. Production of antitoxin was related to being male, high frequency of vaccinations, long period of vaccinations and short period after final vaccination. 3) Among the unbitten group, anti-HR2 was found in high levels with the following factors: high frequency of vaccinations and short period after final vaccination. However among the well-vaccinated group (N = 153), differences in antitoxin levels by vaccination frequency were not recognized.(ABSTRACT TRUNCATED AT 250 WORDS)

Antivenins↗
PubMed1992

Studies on macromolecular activators of phagocytosis from platelets (MAPPs) using anti MAPP murine monoclonal antibodies.

1-MAPP(290 kD) and s-MAPP(150 kD) are glycoproteins which have been shown to be involved in released products from platelets (PRPr) and to activate monocyte and neutrophil phagocytosis via the Fc receptors. Three IgG 2a (kappa) murine monoclonal antibodies against 1-MAPP and nine IgG1 (kappa) antibodies against s-MAPP have been raised. By affinity chromatography using these monoclonal antibodies, active MAPPs were obtained from the supernatant of outdated platelet concentrates in the presence of 60% saturated ammonium sulphate and from PRPr-rich medium, and substantial amounts of non-functioning immunoreactive MAPPs were obtained from the precipitate. Fab fragments of one of the two anti 1-MAPP and of two of the four anti s-MAPP monoclonal antibodies inhibited the function of their corresponding MAPPs, but none of them could inhibit the other MAPPs. 1-MAPP and s-MAPP in the platelets were visualized by indirect fluorescent antibody technique using Fab fragments of the antibodies.

Animals↗
PubMed1992

Pigmented villonodular synovitis (giant cell tumor of the synovium) occurring in the vertebral column. Report of a case.

A case of pigmented villonodular synovitis (giant cell tumor of the synovium) involving the vertebral column is presented. The tumor grew outside the dura and extended to the paravertebral connective tissue, causing sensory and motor disturbance indicative of spinal cord compression. This anatomic location is very rare for lesions of this type, and to our knowledge, this case is only the fifth reported in the English-language literature.

Adult↗
PubMed1992

Multicentric reticulohistiocytosis. Report of a case with electron microscopic studies.

Electron microscopic examination of multicentric reticulohistiocytosis (MR) cells is reported. Most of the cells contained numerous dense bodies which seemed to be lysosomes. Complex interdigitations of the membranes of the adjacent MR cells were distinctive findings. Some of the cells contained myelinated dense bodies in the cytoplasm. Histochemically, the cells were strongly positive for acid phosphatase, alpha-naphthyl butyrate esterase and lysozyme. From these observations, the majority of MR cells appeared to be proliferations of monocyte/macrophage-derived cells. In the synovium, these cells resembled type A synovial cells. Other smaller and fewer cells had many rough endoplasmic reticulum (RER), and these resembled type B synovial cells. In the synovium, MR cells might be formed by proliferation and fusion of synovial cells.

Adult↗
PubMed1992

A light and electron microscopic histochemical study on lectin binding to cells with high metastatic potential in Lewis lung carcinoma.

Lectin binding to tumor cells in tissue sections of nonmetastatic and metastatic murine Lewis lung carcinoma (LLC) was assessed by light and electron microscopy using a lectin-gold technique. Ulex europaeus agglutinin-I (UEA-I) and peanut agglutinin (PNA) showed no binding, whereas concanavalin A (Con A), soybean agglutinin (SBA), Dolichos biflorus agglutinin (DBA), Maclura pomifera agglutinin (MPA), and Ricinus communis agglutinin-I (RCA-I) bound equally to the transplanted sites and metastases. However, wheat germ agglutinin (WGA) bound to metastases more highly than to the transplanted sites and there was a statistically significant difference (P less than 0.01) between the transplanted sites and metastases with regard to pre-embedding method. The tumor cells binding to WGA clearly decreased in number after sialic acid pretreatment and were rich in more well-differentiated organelle. In the bromodeoxyuridine (BrdUrd) labeling in vivo, cell proliferation was greater in the metastatic sites than in the transplanted sites. The above findings suggest that glycoconjugates on the tumor cell surface are altered in the process of metastasis and correlate with metastatic potential and cell proliferation.

Animals↗
PubMed1991

Guinea pig bone marrow cells treated with platelet-activating factor generate factor(s) which affects their DNA synthesis and microbicidal activity.

We have previously reported that platelet-activating factor (PAF) induces proliferation and microbicidal activity of guinea pig bone marrow cells. In the present study, we have found that the conditioned medium of PAF- or nonmetabolizable PAF agonist-treated guinea pig bone marrow cells augmented DNA synthesis and induced microbicial activity of bone marrow cells. A PAF specific antagonist, CV-6209, inhibited generation of the active conditioned medium by PAF. Addition of the PAF antagonist only partially suppressed the augmentative effect of the active conditioned medium on DNA synthesis; this is consistent with the fact that, because of the rapid breakdown, no appreciable amount of PAF remained in the conditioned medium of PAF-treated cells. Although mouse bone marrow cells did not respond to PAF unlike guinea pig cells, their DNA synthesis was significantly enhanced by the conditioned medium of PAF-treated guinea pig bone marrow cells. Thus, some newly generated factor(s) distinct from the originally inoculated PAF seemed to modulate the bioactions of PAF on bone marrow cells. An appreciable amount of PAF was produced by calcium ionophore-treated guinea pig bone marrow cells. These findings indicate that PAF synthesized in guinea pig bone marrow cells induces generation in the cells of some factor(s) which affects proliferation or microbicidal activity.

Animals↗
PubMed1991

Malignant mesothelioma of the tunica vaginalis testis. Report of a case and review of the literature.

An autopsy case of malignant mesothelioma of the tunica vaginalis testis in a 60-year-old man is described. The tumor showed widespread metastasis via the lymphatics, leading to the patient's death five years after diagnosis. A review of 38 previously described cases is also presented. The most frequent presenting symptom was hydrocele. When a hydrocele in association with a paratesticular tumor is present, aspiration cytology should be performed first. In most of the cases with a fatal outcome, the tumors metastasized to the retroperitoneal lymph nodes. Once the diagnosis has been made, lymphangiography or CT scan should be done immediately.

Adult↗
PubMed1991

Dendritic cells in the murine dermis in delayed-type contact hypersensitivity. An ultrastructural and immunocytochemical study.

Dendritic cells within the dermis in the later stages of delayed-type contact hypersensitivity were examined ultrastructurally and immunohistochemically. The immunohistochemical observations were done using monoclonal antibody M1-8, which reacts specifically with murine Langerhans cells and interdigitating cells. Seventeen hours after challenge, infiltrating cells in the dermis included dendritic cells, possibly so-called indeterminate cells, monocytoid cells and Langerhans cells. Immunohistochemically, the indeterminate cells and some monocytoid cells were M1-8-positive. These findings suggest that indeterminate cells are intimately related to Langerhans cells, and that they belong to the mononuclear phagocyte system. M1-8 is a very useful marker for studies on the kinetics of Langerhans cells or indeterminate cells.

Animals↗
PubMed1991

A light and electron microscopical study of membranocystic lesions in a case of lupus erythematosus profundus.

A light and electron microscopical study on membranocystic lesions (MCL) in a case of lupus erythematosus profundus (LEP) is reported. The patient was a 16-year-old female who presented with subcutaneous nodules on both upper arms. The light microscopic features were consistent with LEP, and the result of an immunofluorescence band test supported this diagnosis. A peculiar finding in this case was MCL in the subcutaneous tissue. Ultrastructurally, these were thin membranes without a tubular structure and tortuous thick membranes composed of minute tubules. The lesions were very similar to the fatty tissue changes in membranous lipodystrophy. On the other hand, the basement membranes of the blood vessels were thickened and multilayered, and the lumina were narrowed by endothelial swelling and thickening of the vessel wall. Our findings suggest that the MCL in LEP result from circulatory disturbance of the fat tissue.

Adolescent↗
PubMed1991

One-step double immunolabeling of mouse interdigitating reticular cells: simultaneous application of pre-formed complexes of monoclonal rat antibody M1-8 with horseradish peroxidase-linked anti-rat immunoglobulins and of monoclonal mouse anti-Ia antibody with alkaline phosphatase-coupled anti-mouse immunoglobulins.

A novel one-step double immunolabeling method was elaborated on the basis of the simultaneous application of preformed molecular complexes of two primary antibodies with their specific secondary antibodies labeled with different enzymes. Treatment with a rat monoclonal antibody (MAb), M1-8, pre-coupled with horseradish peroxidase-linked sheep anti-rat immunoglobulins, and enzyme reaction revealed by the 3-amino-9-ethylcarbazole/hydrogen peroxide reaction, resulted in red-brown intracytoplasmic staining of interdigitating reticular cells in the lymph nodes of Balb/c mice. Another molecular complex, made of mouse anti-Ia MAb with alkaline phosphatase-linked rabbit anti-mouse immunoglobulins, applied at the same time and then developed with naphthol AS-BI-phosphate/fast blue BB as substrate, yielded blue surface staining of this cell type in addition to labeling of B-lymphocytes. The method described provides the possibility of relatively rapid double antigen detection where the binding sites of the secondary antibodies are saturated by the specific primary immunoglobulins. This approach seems to avoid nonspecific binding of primary antibodies to Fc receptors, and the unwanted binding of secondary antibodies with cell surface immunoglobulins on B-lymphocytes or with crossreactive primary antibodies used in the other sequence, if the primary antibodies and the tissue are the same or crossreactive animal species.

Alkaline Phosphatase↗
PubMed1991

[Seasonal variation of mortality from cerebro-cardiovascular diseases--effect of ambient temperature on death].

A study to clarify the relationship between seasonal variations in mortality from cerebro-cardiovascular diseases and ambient temperature (maximum/minimum temperature and daily range) was performed using time series analysis, and the role of ambient temperature as an associated factor in cerebro-cardiovascular disease caused death was discussed. The following results were obtained: 1) The seasonality factor associated with the monthly variation for mortality was of greater magnitude for heart diseases more than for cerebral diseases. There was a tendency for mortality from cardiac diseases to decline gradually from February/March to July/August, and to increase in October/November, with a maximum peak in the curve of seasonal variation observed in January/February. 2) The seasonal factor associated with maximum/minimum temperature and daily temperature range showed a stable variation in magnitude from January to December, with temperatures displaying a single peak in August. Daily temperature range was lowest during summer and highest during the late fall, winter or early spring. 3) A statistically significant negative correlation was seen between mortality from cerebro-cardiovascular diseases and seasonality and temperature, while a positive correlation was seen between mortality and daily temperature range, suggesting that low environmental temperatures may operate indirectly as a factor that affects cerebro-cardiovascular disease mortality.

Cerebrovascular Disorders↗
PubMed1991

Thoracic duct cyst of the neck: a case report.

A thoracic duct cyst was excised from the left side of the neck of a 63-year-old woman. It was 6.5cm in length and 3.5cm in width, and was located behind the common carotid artery and jugular vein. The proximal portion of the thoracic duct with a narrowed lumen was in direct contact with the lateral surface of the cyst and the venous angle. The lower end of the cyst extended into the mediastinum to immediately above the aortic arch; the inferior portion of the thoracic duct descended from the bottom of the cyst into the mediastinum behind the aorta. The cyst was unilocular with a fluid content high in triglyceride (3350mg/dl). Its wall was muscular and permeated with lymphatic vessels.

Cysts↗