PubMed Health⌕ Search

Biomedical subjects

H Van Den Berghe

Publications and source records attributed to H Van Den Berghe.

At least 91 records · Page 5Linked to original sources
PubMed1983

Transformed mouse mammary epithelial cells synthesize undersulfated basement membrane proteoglycan.

Proteoglycans deposited in the basal lamina of [14C] glucosamine-labeled normal and [3H]glucosamine-labeled transformed mouse mammary epithelial cells grown on type I-collagen gels, were extracted in 4 M guanidinium chloride and cofractionated over Sepharose CL 4B. The heparan sulfate chains carried by these proteoglycans were isolated by treatment with alkaline borohydride, protease K, chondroitinase ABC, and cetylpyridinium chloride precipitation. Heparan sulfate isolated from transformed cell cultures consistently eluted from DEAE-cellulose at lower salt concentrations and was of smaller apparent Mr when chromatographed over Sepharose CL 6B, than heparan sulfate of normal cell cultures. Experiments using doubly labeled cultures ([3H]glucosamine and [35S]sulfate) demonstrated an approximately 30% reduction in the sulfate/hexosamine ratio in heparan sulfate derived from transformed cultures. Both N- and O-sulfate were decreased. The decreased Mr and decreased sulfation of heparan sulfate upon transformation appear sufficient to explain the altered heparan sulfate/chondroitin sulfate ratios previously observed in these cells. These changes may have implications for the molecular interactions in which these proteoglycans are normally engaged during basal lamina assembly, and cause the poor basal lamina formation displayed by these transformed cells.

Animals↗
PubMed1983

5q- anomaly in a patient with disseminated teratoma.

A 5q- anomaly associated with other chromosome anomalies was found in the infiltrated bone marrow of a patient with a highly malignant teratoma originally located in the mediastinum. There was no evidence of a second malignancy, and it is likely that the 5q- anomaly was, indeed, associated with the malignant teratoma cells.

Adult↗
PubMed1980

Primary amines inhibit recycling of alpha 2M receptors in fibroblasts.

Receptor-mediated endocytosis of alpha 2M-protease complexes by normal human skin fibroblasts in culture was inhibited by the primary amines, methylamine and mono-dansylcadaverine. The latter was effective at a concentration two orders of magnitude lower than methylamine. Several observations indicated an intracellular mode of action of these amines, excluding interference with binding of alpha 2M to the receptor. Inhibition of endocytosis was dissociated from lysosomotropic effects of these amines by kinetic analysis of endocytosis. As opposed to chloroquine and alkaline incubation conditions (pH 8.0), the amines reduced the maximal rate of endocytosis considerably, indicating a reduction in number of available receptors. This was confirmed by measuring binding at 4 degrees C. Continuous recycling of receptors is indicated by the absence of down-regulation of number of receptors under conditions saturating for endocytosis of alpha 2M complexes. We postulate that the primary amines, methylamine and mono-dansylcadaverine, interfere with the recycling of alpha 2M receptors, while the chemical nature of the amines and the conditions under which inhibition becomes apparent point to the involvement of transglutaminase in this recycling.

Cadaverine↗
PubMed1980

Morphological characteristics of epithelial and fibroblastic cells growing out from biopsies of human skin.

Skin biopsies are routinely used to establish fibroblast cultures in vitro, but the ultrastructural properties of the cells during this outgrowth have not received much attention. In the present study, the rate and the nature of the outgrowth of skin biopsy explants were examined in a quantitative and qualitative fashion. Attention was particularly focused on the morphogenetic properties of the various cell types present in the initial outgrowth. The rate of success in obtaining an outgrowth from the explants was slightly dependent on the composition of the medium or the serum. In more than 95% of the cases an initial epithelial outgrowth was obtained. At a later stage the outgrowth became fibroblastic. Remarkable differences in the behavior and ultrastructural properties of epithelial and fibroblastic cells were observed. Incorporation of 3H-thymidine occurred almost simultaneously with the epithelial outgrowth but preceded the migration of the fibroblasts. Under scanning and transmission electron microscopy, the cells of the outermost layers of the epithelium remained flat and polygonal and were covered with small villi during migration. The cells were closely apposed. The germinative cells had a spiky surface and were separated by large intercellular spaces. No basal lamina was formed by the migrating cells. The fibroblasts maintained their shape and smooth surface and reached the substratum under the epithelial outgrowth. They accumulated microfilaments and microtubules in their cytoplasm and were characterized by surface-associated extracellular material. On the substratum, the fibroblasts spreadout. The behavior and ultrastructural properties of these fibroblasts resemble closely those observed on fibroblasts growing-out from aggregates.

Cell Division↗
PubMed1980

Partial distal 12q trisomy.

Two profoundly mentally retarded sisters with partial trisomy for the distal part of the long arm of chromosome 12 (12q24 leads to 12qter) are described. Their anomaly arose as a segregation product of a balanced t(12q-; 17q+) translocation in the mother. Both patients display a similar craniofacial dysmorphism, but lack gross internal or external malformations.

Adult↗
PubMed1979

Langer type of mesomelic dwarfism as the possible homozygous expression of dyschondrosteosis.

A male newborn with the typical Langer type of mesomelic dwarfism is presented. The finding of a variable degree of Madelung's deformity and mesomelic shortening in both parents and in the maternal family tends to support the hypothesis that this type of mesomelic dwarfism may be the clinical manifestation of a homozygous state for dyschondrosteosis.

Diagnosis, Differential↗
PubMed1979

Partial monosomy of the long arm of chromosome 16: a distinct clinical entity?

A 7-month-old male child with a de novo, seemingly balanced reciprocal 5p/16q translocation and karyotype 46,XY,t(5;16) (p14;q21), resulting from a maternal meiotic error, is described. The clinical findings in this patient are strikingly similar to those in the only patient with partial deletion 16q hitherto described, [del(16)(q21)], indicating that during the 5p/16q rearrangement, 16q material was lost and suggesting that partial or total deletion of the long arm of chromosome 16 distal to band q21 is accompanied by a distinct clinical phenotype.

Chromosome Deletion↗
PubMed1979

Chromosome abnormalities in acute promyelocytic leukemia (APL).

Sixteen patients, 15 adults and one child, with APL have been studied cytogenetically; 14 of these had an abnormal karyotype (87%). Eleven of these consistently showed a t(15;17)(q26;q22) structural anomaly, one patient showed a 47,+8 karyotype, one a rearrangement of chromosomes No. 15 and No. 17, apparently different from that in the other patients, and one a No. 17 deletion without a demonstrable translocation. as an additional chromosome change trisomy No. 8 was found in 5 cases and monosomy No. 7 in two. The t)15;17)(q26;q22) structural anomaly is highly characteristic of APL, is found in APL of children and adults, but it is apparently not associated with a clinically different form of APL.

Adult↗
PubMed1979

Chromosomes and causation of human cancer and leukemia: XXXI. Dq- deletions and their significance in proliferative disorders.

Three cases with myeloproliferative disorders associated with Dq- deletions (13q- and 15q-) in their marrow cells are described. In addition, a summary of the experience with 14 cases of Dq- at Leuven, Belgium and the cases in the literature is presented. The findings indicate frequent involvement and susceptibility to deletion of the long arms of chromosomes No. 13 and No. 15 in lympho- and myeloproliferative disorders, thus adding these chromosomes to the list of those involved nonrandomly in human neoplasia.

Adult↗
PubMed1979

A new characteristic karyotypic anomaly in lymphoproliferative disorders.

A new characteristic chromosome anomaly t(11;14)(q14;q32?) in lymphoproliferative disorders (LPD) is described in 4 cases. The extra material was found on a "14 chromosome (14q+) and belonged to the long arm of one "11 chromosome in 3 cases and to the long arm of a "14 in the other case. These cases confirm that the distal end of chromosome 14q may function as a "receptor site," according to the hypothesis of Kaiser-McCaw et al. and also tend to indicate that chromosome "14 may not be unique in showing so-called "donor" and "receptor sites," and that other chromosomes, in casu chromosome "11, may behave similarly.

Adult↗