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Biomedical subjects

H W Harding

Publications and source records attributed to H W Harding.

14 recordsLinked to original sources
PubMed1989

DNA typing and forensic science.

With new typing techniques forensic scientists can characterise individuals at the fundamental level of their DNA. Variation between individuals at this level can be used to discriminate between them. Why this is so and how it is achieved is described in relatively simple terms for legal and medical practitioners who have no formal training in genetics.

Base Sequence↗
PubMed1984

Forensic services in Australia.

Australia is comparable in size to the United States without Alaska, but two-thirds of the continent is desert or semidesert. The population of approximately 15 million is largely centered around major cities near the coastline. There is a federal system of government, and independent forensic services are based in the capital cities of each of the six states and two territories. The need to service both the densely populated urban areas as well as sparsely populated outback regions has created logistic problems. The forensic services in each state and territory have evolved independently and have resulted in a diversity of organizational forms. The organization of forensic services in Australia is described and contact addresses are provided.

Australia↗
PubMed1977

The origin of citrulline-containing proteins in the hair follicle and the chemical nature of trichohyalin, an intracellular precursor.

The present studies have demonstrated that the medulla and inner root sheath cells develop within their cytoplasm a protein that is unique in composition and is present in the trichohyalin granules. The protein is rich in arginine residues, some of which undergo a side-chain conversion in situ into citrulline residues. An unusual Ca2+-dependent enzyme activity distinguishable from cross-linking transamidase has been detected in the hair follicle and will act in vitro on trichohyalin protein as the natural substrate. The conversion in vivo must occur during the time that the medullary and inner root sheath cells move up the follicle and their cytoplasm fills with cross-linked protein containing citrulline. The function of citrulline in these proteins is not understood but its formation is a major process during hair growth.

Amino Acids↗
PubMed1976

Isolation of peptides containing citrulline and the cross-link, epsilon-(gamma-glutamyl)lysine, from hair medulla protein.

The epsilon-(gamma-glutamyl)lysine cross-link has been unequivocally localized in the citrulline-containing protein of hair medulla tissue. This was achieved by the isolation of cross-linked peptides that contain citrulline. The peptides were purified by molecular sieving, cation-exchange and paper chromatography and paper electrophoresis and their amino acid compositions are reported. They were detected by analyzing a large number obtained from a tryptic digest of thermolytic peptides of the medulla protein. There is no evidence at present as to whether the cross-link is in fact inter- or intrachain, or both.

Amino Acids↗
PubMed1976

Arginine transferase activity in homogenates from guinea-pig hair follicles.

The transfer of arginine from tRNA to the amino terminal region of acceptor proteins has been demonstrated in postribosomal supernatant from guinea-pig hair follicle homogenate. The reaction has no requirement for template nucleic acids, ATP, GTP, or Mg++, and is unaffected by high concentrations of cycloheximide. It is concluded that the arginine transfer activity of the guinea-pig hair follicle is due to the action of a soluble enzyme similar to the arginine transferases previously described for other mammalian tissues.

Acyltransferases↗