Biomedical subjects

H W Margraf

Publications and source records attributed to H W Margraf.

17 recordsLinked to original sources

PubMed1982

Detection of antibody to carcinoembryonic antigen by the hemolytic plaque assay.

A modification of the standard hemolytic plaque-forming cell assay is described. The conjugation of a soluble antigen, carcinoembryonic antigen, to the membrane of the sheep red blood cell was accomplished and used as the indicator system to detect antibody-forming cells. The specificity of the assay was determined by cross-reacting studies and competitive inhibition by antigen blocking.

Animals↗

PubMed1977

A trial of silver-zinc-allantoinate in the treatment of leg ulcers.

Three hundred thirty-nine of 400 chronic cutaneous ulcers in 264 patients (including some with multiple or bilateral ulcers of both) were healed with silver-zinc-allantoinate creamed (AZAC 1%). Some of the patients treated had failed to respond to medicated wrappings, saline dressings, and various other therapeutic agents, including antibiotics. In one week of treatment with AZAC 1%, bacterial counts were reduced on the average from approximately 2 X 10(7) to 2 X 10(5) (99%). Silver-zinc-allantoinate cream also demonstrated a wide spectrum of antimicrobial activity. It did not give rise to resistance by the infecting organisms, was nonallergenic, debrided necrotic tissue, and stimulated healthy granulation. Treatment was well tolerated, side effects being limited to a burning sensation in three patients. Most patients cared for themselves at home with minimal interference in their usual daily activities.

Administration, Topical↗

PubMed1977

The use of silver-zinc-allantoin powder for the prehospital treatment of burns.

We describe our experimental studies of a powder formulated to treat serious burn wounds on-the-scene. The wound powder comprises two parts silver-citro-allantoinate, two parts zinc allantoinate and 96 parts pure allantoin. The back skin of 62 rats was shaved and exposed to actively boiling water for ten seconds, resulting in third degree burns of 20% of the total body surface. Immediately, 1 ml of a culture containing 2 X 10(8) Pseudomonas aeruginosa was applied to the burn. The animals were isolated. Of the 30 control rats, six were powdered with allantoin only. Thirty-two rats were dusted with the silver-zinc-allantoin powder within 15 minutes of burning. Cultures were taken at 48 hour intervals. Eighty-seven percent of the control animals died an average of six days postburn. In the treated animals, the mortality was 15%. A mean of 27% of the applied silver (0.35 gm) became incorporated in the eschar. In all control rats, sepsis was detected under the eschar. In treated animals, bacterial concentration fell from an initial average of 5 X 10(4) at 4 hours postburn to 6 X 10(2) at 96 hours.

Allantoin↗

PubMed1975

Fat and solid filtration in clinical perfusions.

The purpose of this study was to define the fat filtration characteristics of a packed polyester wool filter when used alone in the cardiotomy suction (12 cases) and the arterial lines (10 cases) during clinical hemodilution, extracorporeal circulation. The pressure gradient-flow relationship and the efficiency of removal of fibrin microparticles were first ascertained in vitro. Total fat was extracted from the polyester wool with a Soxhlet device. Whole blood and plasma lipids were measured before and after bypass. The results showed a 23 mm. Hg pressure drop across a wool filter with a water flow rate of 5 L. per minute and a 40 to 52 percent efficiency for fibrin microparticles 15 to 250 mu. The total lipid extracted from the cardiotomy filters averaged 376 plus or minus 72 mg. compared with 512 plus or minus 95 mg. in the arterial line filters, although there was no statistically significant difference. Additionally 245 plus or minus 101 mg. of nonlipid material were extracted from the arterial line filters. The postperfusion whole blood contained a higher concentration of lipid than the preperfusion blood when corrected for dilution. It was concluded that significant quantities of solids, two thirds of which were fat, were removed by packed polyester wool filters during total body perfusions. Most of the fat was derived from the cardiotomy suction system. The amount of filtered fat and solids increased with perfusion time. These findings endorse the concept of cardiotomy filtration during extracorporeal circulation.

Blood↗

PubMed1974