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H W Mewes

Publications and source records attributed to H W Mewes.

6 recordsLinked to original sources
PubMed1992

Workshop on two-dimensional gel protein databases.

A workshop on two-dimensional gel electrophoresis (2-DE) protein database, organized by the Committee on Data for Science and Technology (CODATA) of the International Council of Scientific Unions Task Group on Biological Macromolecules, was held at the CODATA Secretariat in Paris on March 9, 1992. Eleven scientists from eight different countries represented various aspects of 2-DE analysis--namely, cellular protein database development and protein microsequencing methodologies. The purpose of the workshop was to explore means of integrating the rapidly expanding body of information on 2-DE resolved proteins from different laboratories. A major proposal emanating from the workshop was the establishment of an intermediary or "relational" 2-DE gel protein database. This intermediary database, which would catalogue pertinent information on 2-DE resolved proteins (experimental source, 2-DE loci, biological information, etc.) could be an adjunct to, and accessed through, the existing international protein sequence databanks. It would function as a pointer for researchers to the individual 2-DE protein databases where primary and more specialized 2-DE data would be housed.

Databases, Factual↗
PubMed1987

A rapid vapor-phase acid (hydrochloric acid and trifluoroacetic acid) hydrolysis of peptide and protein.

A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HCl/trifluoroacetic acid (TFA) vapor mixture. Contamination for the hydrolysis mixture is reduced to low levels (1-3 pmol). Recovery of hydrophobic amino acid is improved. Short reaction times are achieved and rapid removal of acids is facilitated. The reaction temperature is 158 degrees C for reaction times of 22.5 and 45 min with 7 M HCl and 10% TFA containing 0.1% phenol.

Amino Acids↗
PubMed1987

A standardized format for sequence data exchange.

At present there is no agreement upon a standard format for the presentation of sequence data; each of the major sequence databases has adopted their own format. As a result, efforts to pool these data and to develop software to manipulate the data have been hampered. A significant amount of software development time must be invested to handle the incompatibilities among these formats before software to solve biologically interesting problems can be implemented. In principle, the development of a standard format by the database distributors would be the best solution. However, because the databases have invested years of effort in the development of procedures specifically tailored to their own format, they are reluctant to change. Insisting that they convert to a new format would place an extreme burden on the already overtaxed resources of these groups. Furthermore, for certain specialized applications it is more efficient to present the data in nonstandard formats. An alternative solution is presented here. Rather than develop a single standard format for all sequence data, a standardized exchange format has been developed. This format was designed to serve as a common interface between the major formats currently in use. Data can be easily converted to and from it without significant loss of information. This alleviates difficulties inherent in dealing with multiple formats while preserving the local formats of the various databases.

Amino Acid Sequence↗