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Biomedical subjects

H Wakamatsu

Publications and source records attributed to H Wakamatsu.

9 recordsLinked to original sources
PubMed1979

[Diagnostic system for assessment of left ventricular function with 99mTc-albumin (author's transl)].

Diagnostic system is described that obtains a set of serial gated images (SGI) covering the entire cardiac cycles and left ventricular (LV) volume curve (VC) with high temporal resolution (10 msec). The system consists of two functional parts. The one, which is based upon an inexpensive modification of multiformat imaging device, yields SGI. The other, which is based on a minicomputer system, acquires data only from around about LV area and yields LVVC from ECG P wave without the reconstruction of these images. In as little as 5 min result, which is also corrected uniformity of gamma-camera, is given.

Albumins↗
PubMed1978

A new method for preparation of an antiserum to penicillin and its application for novel enzyme immunoassay of penicillin.

A new method for the preparation of ampicillin-BSA conjugate by a three step procedure was developed. The first step is the introduction of a maleimide residue to ampicillin by a cross-linking reagent, MBS. The second step is reductive cleavage of disulfide bonds in BSA. The third step is thioether formation between the introduced maleimide residues and the reduced thiol groups. The obtained ampicillin-BSA conjugated raised an anti-ampicillin serum in rabbits. A new reagent, MPGS, was used for enzyme labelling of ampicillin to avoid immunological cross reaction. Using the enzyme labelled ampicillin and anti-ampicillin serum, enzyme immunoassay of ampicillin was successful in detecting 4 ng to 1 mug. Cross reactivities of anti-ampicillin to ampicillin analogs were studied by the enzyme immunoassay to find that the antiserum is specific to penicillin especially to ampicillin but hardly reacts with cephalosporins or the penicilloic acid derivative of ampicillin.

Ampicillin↗
PubMed1976

Sleep satiation in narcoleptic patients.

UNLABELLED: Polygraphic sleep recording was performed in 20 narcoleptics with one or more of the auxiliary symptoms, 4 narcoleptics with only sleep attacks and 10 normals during one night and into the following day. Total sleep time in the narcoleptics did not differ significantly from that in the normals. Sleep of the narcoleptics with auxiliary symptoms was unstable with frequent awakening. The temporal organization of the REM--NREM sleep cycle was irregular in the narcoleptics with auxiliary symptoms, compared with those in the other two groups. Percentage of Stage 1 was significantly larger in the narcoleptics with auxiliary symptoms than in the other two groups and percentage of Stage 2 was smaller. Percentage of Stage 4 was smaller in the narcoleptics with auxiliary symptoms than in those with only sleep attacks. Percentages of Stages 3 and REM did not differentiate the three groups. Spindle density during Stage 2 did not differentiate the three groups. Sleep onset Stage REM was frequently observed exclusively in the narcoleptics with auxiliary symptoms. Excluding the instances showing sleep onset Stage REM, mean latency of initial episodes of REM sleep in the three groups was shorter after daytime sleep onset than after nighttime sleep onset. In the narcoleptics with auxiliary symptoms, no significant correlation was found between the percentage of Stage REM and clinical findings. CONCLUSIONS: the sleep attacks in narcoleptics are due to an ill-timed, strong tendency to fall asleep (including both REM and NREM sleep), and it is therefore suggested that they are manifestations of their disturbed circadian rhythm of sleep--wakefulness cycle.

Adolescent↗
PubMed1976

[Efficiency of microbe filtration of air filter for rearing germfree animals (author's transl)].

Filtration efficiency of 3 types of air filter frame and cylinder (Fig. 1) was examined by culture of the air obtained from the operating isolators at intervals of 3 to 21 days. The air of isolator was bubled in 30 ml of nutrient broth for 30 or 120 minutes (4approximately15 l/min.) and each of 1 ml of the broth was inoculated into thioglycollate medium (TGC), cooked meat medium (CMM), heart infustion broth (HIB) and glucose peptone water (GPW). The media were incubated for 7 to 14 days at 30 or 25 degrees C. As seen in Table 1, microbes could be effectively removed through all types of filter for over 10 months, though there were a very few positive cases suggesting technically acidental contamination at sampling.

Air Microbiology↗