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H Wakuri

Publications and source records attributed to H Wakuri.

At least 19 recordsLinked to original sources

Intercalated duct cells in the chicken pancreatic islet with special reference to the alloxan administration.

The intercalated duct cells were observed in the A and B islets of the chicken pancreas. These cells adhered with each other by intercellular junctional complexes at the apical side. They had many microvilli projecting into the lumen. Abluminally, they displayed extended slender cytoplasmic processes between islet endocrine cells. Administration of alloxan resulted to denser cytoplasm and a more prominent thickening of cytoplasmic processes of the intercalated duct cells, although the blood glucose levels did not show appreciable changes by the treatment. The intercalated duct epithelial cells appeared clearly as stellate cells. The lysosomes increased in size and number with passage of time after alloxan administration. The present findings may suggest that intercalated ducts are not only anatomically important as a structure passing through the islet but also play physiologically by protecting the islet endocrine cells.

Alloxan↗

Intercalated duct cells in the moorhen (Gallinula chloropus) pancreatic islet.

Intercalated duct cells are present in the alpha and beta islets of the moorhen pancreas. The intercalated duct cells adhere to each other via intercellular junctional complexes on the apical side, projecting many microvilli into the lumen. They also extend slender cytoplasmic processes between islet endocrine cells. These intercalated duct cells appear to have a stellate shape, and to wrap their cytoplasm around the endocrine cells. These observations suggest that intercalated ducts not only pass through the islets, but also play a role in support to islet cells.

Animals↗

What is anatomy for? Considerations on the body as a whole and whole anatomy.

In anatomy today, construction of the body is devoid of the water element, and the framework of the discipline is sectionalized (segmented, specialized, and divided), so that the body is not described in an integrated, harmonious manner. In this paper, the present authors selected the body, body water, and skin as objects of contemplation and deliberated on the history, significance, and resuscitation of anatomy. (1) Living things are dependent on water, and water is a vital component of the body. Therefore, greater consideration should be given to the presence of body water in morphological description of the body. (2) Since anatomy is sectionalized, understanding of the body is dwarfed and distorted. Whole anatomy, which described the body as a whole is the ideal style. (3) The body as a whole should always be placed in the center of anatomy. This should be the ultimate form of teaching anatomy.

Anatomy↗

Electron microscopic study of intercalated duct cells in the chicken pancreatic islet and effects of tolbutamide administration.

Intercalated duct cells are present in the alpha and beta islets of chicken. The intercalated duct cells adhere to each other via intercellular junctional complexes at the apical side, projecting many microvilli and a few cilia into the lumen. They also extend slender cytoplasmic processes between the islet endocrine cells. These intercalated cells appear to have a stellate form, and to wrap their cytoplasm around endocrine cells. Administration of tolbutamide led to increased electron density in the cytoplasm of intercalated duct cells. Lysosomes are present in these cells in various numbers and sizes and tend to increase with time after administration of tolbutamide. These observations suggested that the intercalated ducts not only pass through the islet, but also play a role in supplying islet cells.

Animals↗

Intercalated duct cells in the chicken pancreatic islet.

Intercalated duct cells are present in the alpha and beta islets of the chicken pancreas. The intercalated duct cells adhere to each other via intercellular junctional complexes at the apical side, projecting many microvilli and a few of cilia into the lumen. Abluminally, these cells extend slender cytoplasmic processes between islet endocrine cells. These intercalated duct cells appear to have a stellate form, and to wrap around endocrine cells with their cytoplasm. These observations suggest that intercalated ducts not only pass through the islet, but also play a role in supplying islet cells.

Animals↗

Developmental-anatomical observations on cleavage line patterns of the skin in Chinese miniature pigs.

In twelve Chinese miniature pigs (CMP), of both sexes and ranging from newborn to 5 months of age, the postnatal developmental features of the cleavage lines of the skin of the whole body were examined by the stab-wound method, using a metal probe with a sharp conical point. 1). The CL patterns of the skin in the 5-month-old CMP were largely similar to those of adult Landrace-strain pigs (Wakuri et al., 1993). 2). At each monthly stage, the CL of the skin of the inter-ocular area, medial ocular angle, umbilicus, preputial orifice and anus presented a convergent arrangement. An annular pattern was found in the skin around the eye, the base of the ear, and the vulva. 3). Some alterations in CL patterns were seen during the general and regional growth of CMP, as shown in the skin of the lateral side of the trunk, the prepuce and the scrotum.

Abdomen↗

Prolactin in the Cestodes, Taenia solium and Taenia hydatigena: an immunocytochemical study.

The demonstration of prolactin in cestodes, Taenia solium and Taenia hydatigena, was investigated by using immunocytochemical method. PRL immunoreactivity was widely distributed in the CNS and PNS in the worms. In the scolex PRL-IR perikarya occurred in the cerebral ganglia, around the basic area of the sucker and the rostral border region between the rostellum and the suckers with strong immunoreaction, distinctly in Taenia solium. A number of PRL-IR nerve fibres were observed in the main nerve cords, and a few fibres in the transverse nerve commissure. The holdfast musculature of the scolex was innervated by the PRL-IR fibres. A large number of PRL positive cells with processes and nerve plexuses were seen in the ootype/Mehlis' gland complex, oviduct, the duct of uterum, seminal receptacle and its associated duct, conspicuously in Taenia hydatigena. PRL-IR was evident in the wall of vas efferens of tests and vas deferens. A few PRL positive cells occurred in the medullary parenchyma amongst testes and sent their varicose processes to terminate to the testes. On the wall of the main excretory duct a few PRL-IR cells and fibres were seen.

Animals↗

Ultrastructural study of neurosecretory cells in the nervous system in the cestode (Taenia hydatigena).

The neurosecretory cells in nervous system of the Taenia hydatigena was studied light- and electron-microscopically. On the basis of the cytological structure they were divided into two types: type I neurosecretory cells, which containing large dense-cored vesicles, small elongated mitochondria and a large amount of free ribosomes, were located mainly in the central and peripheral nervous system; type II neurosecretory cells were characterized by their moderate amount of free ribosomes, endoplasmic reticulum which contacted with the membranes of perikarya, large ovoid mitochondria, large dense-cored vesicles and their localization in the musculature near serve profiles. The synaptic and nonsynaptic contacts i.e. omega figure and exocytosis release sites were seen in the neuropile, musculature and excretory ducts. The present findings suggest that neurosecretory cells in nervous system may play an important integrative roles of both neuronal and endocrine in the flatworm.

Animals↗

Microscopic anatomy of the equine skin with special reference to the dermis.

We have observed and re-evaluated the histology of the skin of the horse, using samples from four Thoroughbreds. The skin was composed of the usual three components: epidermis, dermis and subcutis. In particular, the dermis was found to have three fibrous components: a papillary layer, a reticular layer and a cordovan-leather tissue layer. The cordovan-leather tissue layer was subdivided into a superficial main layer and a deeper accessory layer. The superficial main layer was thick, and present in all of the skin samples. The deeper accessory layer was found in the dorsal and dorso-lateral parts of the neck and trunk and in the extremities. Hair bulbs and sudoriferous glands did not extend into the cordovan-leather tissue layer and subcutis.

Animals↗

Immunocytochemical evidence for the presence of prolactin in the plerocercoid of Ligula intestinalis (Cestoda: Pseudophyllidea).

Immunoreactivity to prolactin in the nervous system of the plerocercoid of Ligula intestinalis was demonstrated by immunocytochemical method. Numerous PRL immunoreactive perikarya with long varicose fibres were observed in the peripheral nervous system in the worm, mainly in the transversal muscle layer and medullary parenchyma of the midbody. A few fibres were found in the main nerve cords of the central nervous system. PRL positive neurons sent their processes to associate with the main nerve cords. The immunostaining terminals appeared in the subtegument region in the lateral border of the plerocercoid. The result indicates that PRL immunoreactivity is well-developed in the plerocercoid of the cestode. The significance of the localization of prolactin in the worm is discussed.

Animals↗

Development of cleavage line patterns in the skin of the ovine fetus.

In fifty six ovine fetuses of both sexes, ranging in length from 20 mm to 41 cm crown-rump length, the developmental features of the cleavage lines of the skin over the whole body were examined by the stab-wound method using a metal probe with a sharp conical point. 1). Cleavage lines were observed as early as the 20 mm fetal stage. During each fetal stage, the various regions and areas of the ovine body were represented by individual cleavage line patterns. 2). The cleavage line patterns changed as the occurred the various structures elongated or thickened during fetal growth. 3). Among the fetuses, similar cleavage line patterns recurred in particular regions or areas, e.g., the flank, the antebrachium, the crus and fore- and hind-digits. 4). This recurrence of cleavage line patterns reflected the alterations with occurred with the growth of the whole body, in accord with the so-called functional-morphological rhythm of development.

Animals↗

Anatomical observations on cleavage line patterns of the skin in adult pigs.

In six pigs from 7 months to 3 years of age the cleavage line pattern of the skin was examined in the whole body by a thick metal probe with a sharp conical point. Directionality of the cleavage lines was determined from the relationship of the line of the long axis of each physical structure. The cleavage lines of adult pigs were compared with those of young pigs (Wakuri, 1967). The cleavage lines of the ear, neck, trunk, and free extremities of appendage were quite similar in adult and young pigs. Those of the buccal, interscapular and sacral regions, umbilicus and scrotum showed slight differences. The most noticeable directional variability in cleavage lines appeared in the masticatory region, arms and legs of adult pigs. Cleavage lines were found to be transverse in arrangement in the masticatory region, auricular root, neck, trunk, tail and legs. They showed an annular pattern around the preputial orifice and in the arms and anogenital region.

Animals↗

Histochemical study on the gland of pelvis renalis in the horse.

The glands of pelvis renalis in adult and fetal horses were examined by histochemically. The glandular terminal was divided into two types, intraepithelial gland (IE) and extraepithelial gland (EE) by their locations and histochemical characters. Both glands were composed of mucous cells. The former lay in the transitional epithelium and were stained reddish with galactose oxidase-Schiff (GOS) and mild oxidation-Schiff (MOS), bluish purple with periodic acid-cold thionine Schiff-NaOH-PAS (PCP). The latter distributed in the lamina propria, but was not discovered in the fetus. They were stained weak or negative with GOS and MOS, reddish purple with PCP. Their difference of histochemical character might be reflect to chemical structure of the sialic acid. These mucous cells may cope with the urine contents as hippuric acid.

Animals↗

Considerations on the morphology and terminology of the organs.

In anatomy, the morphological features of organs are captured in a variety of ways, and this allows wide interpretations of the terminology for organs. However, the present terminology is considered to include terms that require re-evaluation for appropriate ranking of organs. Organs should be understood in a proper perspective of their anatomical hierarchy and architectural characteristics. I consider that organs should be classified morphologically by the terminology of "organelle" on the cytological level, "sub-organ" on the histological level, "organ" on the macroscopic anatomical level, and "organ system", which may also be expressed as "apparatus" or "organa", on the level of systematic anatomy. I strongly demand that N.A.V.-N.H. (1983) acknowledge this hierarchial order and adopt the general term "organum".

Anatomy↗

Binocular dissecting microscopic studies on the density of toruli tactile in the La Mancha goat.

The Merkel cells and associated nervous terminal plates have been called toruli tactiles (TT). These structures can be ubiquitously seen on the shaved hairy skin after staining with a methylene blue solution. Four La Mancha goats of both sexes were examined for the density of TT. TT were visually located under a binocular dissecting microscopic (14x) and then the number of TT in the field was directly counted. The density of TT in different corporal areas of the body surface was estimated from the mean of several two-cm square samples of skin. The results obtained are as follows: The mean number per two-cm square on the whole body surface ranged from 10.06-11.36 in the male specimen and from 12.98-14.32 in the female specimen. The density of TT was rather high in the temporal and buccal areas, on the neck, chin and cranial breast, and on the lateral surface of the arm, thigh and leg. Density was moderate in the fronto-parieto-occipital area (female), on the loin and buttocks, in the upper costal, lower caudo-costal, xiphoid-umbilical and umbilical-pubic areas, and on the lateral surface of the forearm. TT were absent or very few in number on the nose and chin, the scrotum, caudal-pubic area of males, the udder, the vulva, and the digitorum manus et pedis.

Animals↗

Density of Toruli Tactiles in the goat (Japanese saanen).

Toruli Tactiles (TT) were clearly observed to be stained with methylene blue solution, and the density of distribution of TT on the body surface was measured in the goat. TT in the goat were present on the hairy skin as papilla-like structure of about 200 to 300 microns in plane-diameter. A high density of TT on the body surface occurred in the areas from the lateral portion of the arm to forearm, and from the thigh to leg. On the other hand, areas of low density of TT were present in the facial-cranial region except for the buccal-masseteric area, cranial-breast, perineum (male), axilla, inguen, carpus-metacarpus, and tarsus-metatarsus. TT were rarely observed in the ears, on the nose, around the mouth, perianal parts, palms and soles.

Animals↗

Arnold-Chiari malformation and associated anomalies in a dicephalic newborn calf.

A full-term female newborn calf of the Japanese black short-horn breed which was two-headed (dicephalus) and had two incomplete necks was carefully dissected. The calf was one of twins, the other being a normal male. There was no record of reproductive problems with the cow. The left and right heads were of almost the same size and shape. Each head had a nose, mouth, eyeballs, and ears. The brains were very congested. That is to say, in both the left and right brains, the longitudinal fissure was unclear, and these was narrower pattern in the cerebral gyri. The surfaces of the two brains displayed poly- or microgyria-like features. The cerebellar gyri ran longitudinally among the occipital lobes of the cerebral hemispheres. The caudal half of the cerebrum, the cerebellum and the brain stem were distorted ventrally at an angle of approximately 80 degrees. Twisting of the brain stem was observed in the right brain. The end of the medulla oblongata and a tongue-like process of the cerebellum extended through the foramen magnum of the skull into each enlarged vertebral canal (Arnold-Chiari malformation). They extended under the arch of the each 2nd cervical vertebra. The 4th ventricle was also displaced and irregularly dilated. The two necks were conjoined in the single 3rd cervical vertebra at an angle of approximately 45 degrees to each other. The one trunk was slightly distorted. The ribs revealed the presence of a left 14th extra tib. In the lumbar region, spina bifida was apparent. The pelvis was distorted asymmetrically. The tail was short. The viscera appeared as one set, but the rectum and anus were duplicated. Although the calf displayed female features, the vagina was undeveloped. The four limbs appeared normal and the joints moved freely.

Animals↗