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Biomedical subjects

H Weir

Publications and source records attributed to H Weir.

9 recordsLinked to original sources
PubMed2000

Second primary ovarian cancer among women with cancer.

PURPOSE: Some studies suggest women with certain types of cancers are at increased risk for ovarian cancer. This study assessed the risk of second primary ovarian cancer among U.S. women who have cancer by anatomic site, age, race, and time since diagnosis of the first primary cancer.METHODS: We analyzed data from SEER cancer registries for women diagnosed with invasive cancer between 1973 and 1996. Person-years were accumulated from 2 months after initial cancer diagnosis to date of ovarian cancer diagnosis, death, loss to follow-up, or end of follow-up, December 31, 1996. The expected number of cases was obtained by multiplying 5-year age and calendar year interval specific ovarian cancer rates by the accumulated person-years at risk. We calculated the risk (observed [O]/expected numbers [E]) of second primary ovarian cancer by cancer site and age (<50 years, >/=50 years), race (all, white, black), and time since first cancer (0-4, 5-9, 10-14, 15-24 years). Statistical tests and 95% confidence intervals (CI) were based on the assumption of a Poisson distribution.RESULTS: A significant increased risk of ovarian cancer was found for women aged <50 years at time of diagnosis with melanoma (O/E = 3.5, 95% CI = 2.1-5.5) and cancer of the breast (O/E = 6.0, 95% CI = 4.9-7.2), cervix (O/E = 4.2, 95% CI = 2.6-6.3), corpus uteri (O/E = 11.91, 95% CI = 7.3-18.4), colon (O/E = 17.9, 95% CI = 11.1-27.3), and ovary (O/E = 4.9, 95% CI = 2.7-8.2); no increased risk was found for women aged >/=50 years. Ovarian cancer risk remained elevated following all of these first primary cancers 5-9 years after diagnosis; for women with breast and colon cancer, risk remained elevated 15-24 years after diagnosis. A significant increased risk was found for all of these cancers among white women <50 years at diagnosis; risk was increased among black women <50 years with cancer of the breast, cervix, and colon.CONCLUSIONS: We found an ovarian cancer risk higher than expected for women with certain types of cancer; however, the risk was limited to women <50 years of age.

Journal Article↗
PubMed1994

Hydrochloric acid denaturation of colorectal tumour tissue infiltrated with bromodeoxyuridine.

The thymidine analogue, bromodeoxyuridine, is substituted into DNA during DNA synthesis and can be used to identify those cells which have passed through the S-phase of the cell cycle. Incorporated bromodeoxyuridine can be detected using anti-bromodeoxyuridine monoclonal antibodies which bind to the exposed bromodeoxyuridine in single-stranded DNA after a suitable denaturation step. Hydrochloric acid is the most commonly employed denaturation agent in bromodeoxyuridine monoclonal antibody methodologies. This preliminary study was to validate the hydrochloric acid denaturation step for colorectal tumour tissue infiltrated in vivo with bromodeoxyuridine. Standard immunohistochemistry and flow cytometric techniques using Bu20a were employed across a range of hydrochloric acid concentrations. Although high labelling indices were achieved using acid concentrations of 0.10 M HCl, the optimal hydrochloric acid concentration was not the same in all tumours. Sensitivity of DNA to hydrochloric acid denaturation should be carefully considered in bromodeoxyuridine methodologies using the monoclonal antibody Bu20a.

Antibodies, Monoclonal↗
PubMed1981

Lidocaine levels during CPR: differences after peripheral venous, central venous, and intracardiac injections.

Drug administration via peripheral vein, central vein, and intracardiac routes is generally assumed to be equally effective during cardiopulmonary resuscitation (CPR). Experiments were performed in an animal model to evaluate this assumption. Twelve mongrel dogs weighing greater than 20 kg were studied. Arterial blood pressure and electrocardiogram were monitored continuously. Cardiac outputs were evaluated before CPR to determine control. After thoracotomy and fibrillation of the heart, cardiac massage was started and the rate of compression adjusted to give 30% of control cardiac output. A lidocaine bolus of 1.5 mg/kg was given via peripheral vein in four dogs, central vein in four dogs, and intracardiac (left ventricle) in four dogs. Drug levels were sampled through an aortic catheter at the level of the coronary artery ostia every 20 sec for five min, every 30 sec for 10 min, and every 60 sec for 15 min. There was no significant difference in the appearance of effective levels or time of peak levels in the three groups. The peak levels were highest in the central venous group, while peripheral venous and intracardiac peak levels were 63% and 31%, respectively, of the central venous peak. Duration of effective levels was 20 min in the intracardiac group, 14.5 min in the central venous group, and 9.6 min in the peripheral venous group. Further studies are needed to determine whether changes are needed in drug administration during CPR in man.

Animals↗