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Biomedical subjects

H Weissbach

Publications and source records attributed to H Weissbach.

At least 181 records · Page 10Linked to original sources

Translation in vitro of total nuclear RNA from HeLa cell nuclei infected with adenovirus 2.

Total heterogeneous nuclear RNA from HeLa cells infected with adenovirus for 18-20 hr stimulates amino acid incorporation into protein in a cell-free system from Ehrlich ascites tumors. This stimulation of protein synthesis by the nuclear RNA requires intact nuclei. The role of nuclei in this system is unknown, but evidence is presented that the nuclei are involved in the conversion of high-molecular-weight RNA to low-molecular-weight species. Some of the newly synthesized polypeptides appear to resemble the virion polypeptides.

Adenoviridae↗

Multiple forms of elongation factor 1 from calf brain.

Heavy and light forms of elongation factor 1 (EF-1) from calf brain have been partially purified. The heterogeneous heavy species (EF-1(H)) with molecular weights of 2.5 x 10(5) to over 1 x 10(6) appears to be a complex or aggregate of the light form of the enzyme (EF-1(L)); the latter has a molecular weight of between 50,000 and 60,000. EF-1(H) but not EF-1(L), contains significant amounts of free and esterified cholesterol. Although EF-1(H) and EF-1(L) are both active in aminoacyl-tRNA binding to ribosomes, EF-1(L) reacts with GTP and aminoacyl-tRNA more efficiently than EF-1(H).

Ammonium Sulfate↗