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H Wintz

Publications and source records attributed to H Wintz.

22 records · Page 2Linked to original sources

Location and nucleotide sequence of two tRNA genes and a tRNA pseudo-gene in the maize mitochondrial genome: evidence for the transcription of a chloroplast gene in mitochondria.

We report the nucleotide sequence of three tRNA genes from maize mitochondria. The genes are located in two BamHI fragments, 3.55 and 5.7 kb long, adjacent to the S2 sequence in the maize mitochondrial genome. On the 3.55 kb BamHI fragment, we have characterized a tRNA(Cys)(GCA) gene. A strong sequence homology of this tRNA(Cys)(GCA) gene with its chloroplast counterpart in wheat suggests that it may be part of a chloroplast DNA insertion into the mitochondrial genome. This gene has been found to be transcribed in the mitochondrion. Two tRNA genes are located on the 5.7 kb BamHI fragment, separated from each other by 250 bp. One is a mitochondrial tRNA(Ser)(GCU) gene. The other, a non-transcribed tRNA(Phe)-like gene, is interrupted by a 49 base-pair inserted DNA sequence in the variable loop and has a Leu (UAA) anticodon.

Base Sequence↗

Presence of a chloroplast-like elongator tRNAMet gene in the mitochondrial genomes of soybean and Arabidopsis thaliana.

The nucleotide sequence of elongator tRNA(Met) genes from soybean chloroplast and mitochondria and Arabidopsis thaliana mitochondria have been determined. The mitochondrial tRNA(Met) genes from soybean and A. thaliana are identical, and they differ from the soybean chloroplast tRNA(Met) gene by only four nucleotides. Analysis of the flanking regions indicates that the mitochondrial tRNA(Met) gene is not present on a large chloroplast DNA insertion in the mitochondrial genome, but it suggests that they have a common origin. Comparison of the three genes and the evolutionary implications are discussed.

Base Sequence↗

Nucleotide sequence and transcription analysis of the gene coding for subunit III of soybean chloroplast proton-translocating ATPase.

The gene coding for subunit III of CF0-ATPase complex (atpH gene) has been localized on the soybean chloroplast genome by heterologous hybridization with the atpH gene of wheat chloroplast. The gene, 243 bp in length, is located in a 7.5-kb BamHI fragment generated from the 14.8-kb PvuII fragment in the large single-copy region of the chloroplast genome. The nucleotide sequence of the gene has been determined. The deduced amino acid (aa) sequence shows 2 aa changes relative to its homologue from wheat and spinach and 1 aa with respect to that found in tobacco chloroplast. The gene is apparently transcribed independently as an 850-nt transcript.

Amino Acid Sequence↗