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Biomedical subjects

H Wolf

Publications and source records attributed to H Wolf.

At least 703 records · Page 39Linked to original sources
PubMed1975

Attempts to detect virus-specific DNA sequences in human tumors. III. Epstein-Barr viral DNA in non-lymphoid nasopharyngeal carcinoma cells.

Fourteen tumors of the nasopharyngeal region were analyzed for the presence of Epstein-Barr virus-specific DNA by DNA-cRNA hybridization. These data were compared to the histology of the respective tumors and the seroreactivity of the tumor-bearing patient against EBV-related antigens. With one exception, all tumor pieces containing nasopharyngeal carcinoma cells hybridized significantly with EBV-cRNA. Tumors of predominantly epithelial morphology annealed in the highest range. In situ-hybridization of freeze sections from a tumor containing almost equal amounts of tumor cells and lymphocytes revealed hybridizing DNA within nuclei of non-lymphoid cells. Although these data do not exclude the presence of EBV-DNA within lymphoid cells, they clearly demonstrate that in nasopharyngeal carcinomas the vast majority of EBV-specific DNA rests within non-lymphoid cells.

Carcinoma↗
PubMed1975

The significance of histological changes in serial prostatic biopsy specimens.

Study of the histological appearance of randomly chosen areas of hyperplastic prostatic glands revealed that such areas are highly variable in appearance. Any such area of the size comparable to biopsy specimens does not give a representative picture of the entire gland. It is not possible from the appearance of single, serial biopsy specmens to draw any conclusions on the effect of hormonal treatment of the hyperplastic prostrate.

Biopsy↗
PubMed1975

Extracorporeal immunoadsorption of circulating specific serum factors in cancer patients.

Circulating serum factors have been said to abrogate the effects of immune response in cancer, i.e. "blocking" and "antigenic inhibition". The aim of this investigation was to isolate such specific factors in a purified and native state. F(ab)2 fragments isolated from hypernephroma were insolubilized on the surfaces of an extracorporeal perfusion chamber which was inserted into the circulation by means of an arterio-venous shunt. As a result, 3 proteins not present in normal serum were isolated and eluted for further study. In immunoelectrophoresis the 3 proteins were specifically precipitated by heterolgous (rabbit) antihypernephroma serum but not by anti-serum directed against normal serum components. Moreover C9 components, C3 activator and C3 were isolated in the chamber, the latter complement factor in large concentrations. This further sustained that specific antigen-antibody reactions had occurred in the chamber. One of the 2 patients studied were perfused for 60 h and 40 min. During this period 450 litres of blood were brought into intimate contact with the immunoadsorbent. Proteins in amounts sufficient for immunochemical analysis were isolated within 3 h.

Adenocarcinoma↗
PubMed1975

Sudden massive hemorrhage after renal transplantation.

Five cases of sudden massive hemorrhage after renal transplantation are reported. It is supposed that an infective arteritis with damage of the artery wall caused the observed disruption of the arteries. Prompt ligation far from the infected site is recommended as necessary to prevent recurrence of the bleeding and to afford the best possibility for survival.

Adult↗
PubMed1975

[Continuous negative pressure (cnp) in infants with idiopathic respiratory distress syndrome (irds) (author's transl)].

13 unselected premature infants with idiopathic respiratory distress syndrome (IRDS) have been treated with continuous negative pressure (CNP). A chamber was applied to the infants' body, so that the head remained outside in normal atmospheric pressure. 4 infants died during the period of treatment. 6 out of 13 differently treated IRDS-infants died over the same 6 months period. In comparison 18 out of 27 infants with IRDS died the previous year, during the same period. The frequency of IRDS in both observation periods was the same, as was the therapy of acidosis and hypoxia. The infants responded to CNP with decreased respiratory frequency, lessening of "grunting" and return of a pink skin color. Pathological examination of 3 out of 4 deceased infants in the study group revealed both atelectases and some degree of interstitial emphysema and widened capillaries. No intravascular hyaline thrombi were seen. The remaining infant died with clinical signs of coagulopathy (no autopsy). Of the 9 surviving infants in the study group one developed a hydrocephalus and subsequent mild psychomotor developmental retardation. We conclude that the CNP therapy in the chamber is an asset to the treatment of early and medium-severe cases of IRDS. We believe, that early application might prevent development of severe IRDS. The method is not recommended for severe cases.

Carbon Dioxide↗
PubMed1975

[Concentration and subcellular distribution of iron and copper in the pig liver. II. Studies on the livers of porkers and swine for slaughter with regard to the addition of copper sulfate to the diet].

In the livers of weaners, an average concentration of storage iron of 69.1 +/- 47.6 mug, and in those of pigs for slaughter, 326 +/- 54 mug/g tissue was measured. Addition of 250 ppm of copper as CuSO4-5H2O to the animals' food led to a significantly lower concentration of 248 +/- 63-5 mg Fe/g liver in pigs for slaughter. Of the storage iron, 66-1% for weaners, 79.2% for pigs for slaughter, and 76% for animals given Cu-supplemented food were localized in the microsomal fraction and in the cytosol. The copper concentration in the liver was 5.2 +/- 0.6 mug/g in weaners, 8.1 +/- 2.4 mug/g in pigs for slaughter and 120 +/- 83 mug/g in animals kept on a Cu-supplemented diet, Of these, 72.6% (pigs for slaughter) and 71.9% (weaners) came to the microsomal fraction and the cytosol, whereas addition of 250 ppm copper to the food ration enhanced the proportion in the nuclear fraction from 7.7 to 29.5%, and in the mitochondrial fraction, from 19.8 to 45.1%. The percent distribution of the copper on the subcellular fractions depends on the copper concentration in the liver: in the range of 5-12 mug/g tissue, increased copper concentration in the liver caused preferential localization of the copper in the cytosol, whereas between 25 and 330 mug/g tissue the copper was stored subcellularly in the following order: mitochondrial, nuclear, cytoplasmatic and microsomal fractions.

Animals↗
PubMed1975

Studies on tryptophan metabolism in Danish bladder cancer patients.

Urinary excretion of metabolites of the kynurenine pathway before and after a loading dose of 2 g L-tryptophan was studied in 31 bladder cancer patients from the Copenhagen area. Only 2 patients (6 per cent) showed abnormal tryptophan metabolism, and both patients excreted increased amounts of only 3-hydroxykynurenine after a loading dose of 2 g L-tryptophan. The percentage of bladder cancer patients with abnormal tryptophan metabolism is compared with the percentage found in other parts of the world. It is suggested that these findings may indicate that tryptophan metabolites do not play a major role in the genesis of bladder cancer among bladder cancer patients from the Copenhagen area. Other etiologic factors should be looked for.

Denmark↗
PubMed1975

[The requirements of vitamin D (cholecalciferol) and of 25-hydrocalciferol (25HCC) in premature children during their early life (author's transl)].

The necessary daily amount of vitamin D3 in premature infants was calculated by Hövels and his group as being 25 mcg (1000 I.U.). This was in agreement with our own investigations. Without vitamin D3, however, the incidence of rikkets in premature and small-for-dates newborns occurred usually at the beginning of the second month of life. Clinical signs of rickets are still absent at this time, while an increased activity of the serum alkaline phosphatase signals the beginning of the illness. Three groups of prematurely born infants received up to 50 days daily oral doses of 12.5 mcg vitamin D3 (=500 I.U.), 25 mcg vitamin D3 (=1000 I.U.) or 9 mcg 25-hydroxycholecalciferol (25-HCC), respectively, while one untreated group served as control. No differences were noted between the four groups in the serum values of calcium and phosphorus. The activity of the alkaline phosphatase tended to be higher in the untreated and the 500 I.U. groups. The differences were prouved by the chi2-test to be significant (see table 2 and 3). It appeared that 9 mcg of 25-HCC prevented the rise in alkaline phosphatase slightly better than 25 mcg of vitamin D3 (1000 I.U.). On a mcg base 25-HCC seems three times as effective as cholecalciferol. One could speculate about a delayed hydroxylation of cholecalciferol (vitamin D3) to 25-HCC.

Age Factors↗
PubMed1975

Alteration of hemodynamics, blood gases and lung morphology in bromocarbamide intoxication (animal experiments).

The reaction to animals in bromocarbamide intoxication with respect to blood pressure, resistance in the systemic and pulmonary circulation, cardiac output and arterial blood gas values is presented and discussed in reference to morphological changes in the lung. The animals die due to peripheral circulatory failure if paralysis of respiration is prevented by means of artificial ventilation. The observed morphological lung changes are not the cause of death.

Animals↗
PubMed1975

[Fat-emulsions and acid-base balance in neonates of low weight at birth (author's transl)].

The influence of a single intravenous infusion of triglycerides (1 g triglyceride per kg body weight) on the acid base equilibrium was investigated in 16 newborn infants with low birth weight during the first days of age. No changes of pH, PCO2 and standard bicarbonate, base excess, buffer base and actuelle bicarbonate, respectively, were observed. Fat emulsions can be used, therefore, without hazards in the parenteral feeding of newborn infants.

Acid-Base Equilibrium↗