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Biomedical subjects

H Y Ren

Publications and source records attributed to H Y Ren.

8 recordsLinked to original sources
PubMed2006

Determination of steroid estrogens in wastewater treatment plant of a controceptives producing factory.

Steroid estrogens such as estrone (E1), 17beta-estradiol (E2), estriol (E3), and 17alpha-ethynylestradiol (EE2) have been suspected to be the main contaminants, which can affect the endocrine system of animals. Many authors have investigated these chemicals in the domestic wastewater treatment plants (WTP). However, wastewater from industries producing steroid contraceptives has not got ample attention. From the environmental point of view, the four steroids are very significant because even very low concentrations (ng/L) can cause reproductive disturbances in human, livestock and wildlife. The main purpose of the present investigation was to develop an analytical method for the determination of the four steroid estrogens present in WTP of a pharmacy factory, mainly producing contraceptive medicine in Beijing, China. Analysis was performed by solid-phase extraction (SPE) system and liquid chromatography combined with tandem mass spectrometry (LC/MS/MS). The average recoveries from effluent samples ranged from 88% to 103% and the precision of the method ranged from 9% to 4%. Based on 0.5-L wastewater samples, the limit of quantification (LOQ) was determined at 0.7 ng/L for E1, 0.8 for E2, 0.9 ng/L for E3, and 0.5 ng/L for EE2 in influent, and 1.0 ng/L for E2 and EE2, and 2.0 ng/L for E1 and E3 in effluent. In the influent samples, average concentrations of 80, 85, 73 and 155 ng/L were determined for E1, E2, E3 and EE2, respectively, showing that they were removed in this WTP to the extent of 79, 73, 85 and 67%, respectively.

China↗
PubMed2005

Estimation of genetic parameters for lactational milk yields using two-dimensional random regressions on parities and days in milk in Chinese Simmental cattle.

A two-dimensional random regression model with regressions on days in milk (DIM) and parity number was applied to lactational milk yields in Chinese Simmental cattle. Random regressions were fitted for additive genetic and permanent environmental effects using a two-dimensional polynomial on DIM and parity number. A total of 4340 lactational milk yields from Chinese Simmental cattle which calved between 1980 and early 2000 were used in this study. Variance components were estimated using Bayesian methodology via Gibbs sampling. Variances of random regression coefficients associated with all terms of the polynomials were significant. A covariance function showed that heritabilities of lactational milk yields between 200 and 400 DIM over parities varied between 0.25 and 0.45. Heritabilities of 305-day milk yields from 1st to 6-8th parities were 0.28, 0.30, 0.32 0.32, 0.32, and 0.31, respectively. Ratios of permanent environment variances to total variances at each DIM were greater than corresponding heritabilities. Generally, genetic correlations were higher between lactational milk yields with similar DIM and parity number.

Animals↗
PubMed1996

[Clinical and experimental observation of xilixin granule combined with radiotherapy in treating malignant-tumors].

Thirty six patients of pulmonary or nasopharyngeal carcinoma were treated with Xilixin granule (XLXG) combined with radiotherapy and compared their efficacy with that of 31 patients treated by Zhenqi Fuzheng granule combined with radiotherapy for control. Results showed that the symptoms of Yin Deficiency syndrome in treated group were obviously improved, the leucocyte decreased by 5.6%, while in control group it reached 25.8%, the 3 year survival rate was significantly higher in treated group (75.0%) than that in control group (51.6%). Animal experiment revealed that XLXG had the effects of tumor inhibition, it could increase white blood cells, platelets and hemoglobin of patients, especially in using large dosage. These results suggested that XLXG have some protective effect against radiotherapeutic damage in patients with malignant tumor.

Adolescent↗
PubMed1994

Erythropoietin induces tyrosine phosphorylation and activation of phospholipase C-gamma 1 in a human erythropoietin-dependent cell line.

Erythropoietin (Epo) is the major regulator of the proliferation and differentiation of erythroid precursors through interaction with its receptor (Epo-R). Although Epo-R lacks a tyrosine kinase consensus sequence within its intracellular domain, the addition of its ligand to Epo-responsive cells, UT-7/Epo, induces the rapid and transient tyrosine phosphorylation of 145-, 130-, 80-, and 40-kDa cellular proteins. Tyrosine phosphorylation of these proteins occurred dose- and time-dependently. We showed that the tyrosine phosphorylated 145- kDa protein is identical to phospholipase C-gamma 1 (PLC-gamma 1). Tyrosine phosphorylation of this protein is detectable within 30 s and almost reaches the maximum at 1 min. This can last up to 10 min and declines thereafter. Additionally, in Epo-stimulated cells, PLC-gamma 1 become physically associated with 80- and 40-kDa proteins which have been tyrosine-phosphorylated in response to Epo. The activity of PLC-gamma 1 was also investigated using inositol 1,4,5-trisphosphate (Ins-P3) as an indicator. We found that stimulation of UT-7/Epo cells with Epo induces a significant accumulation of Ins-P3. This effect is dose-dependent and occurs very rapidly. The production of Ins-P3 can explain the Epo-induced mobilization of calcium from intracellular stores in these cells. These results demonstrate that Epo induces tyrosine phosphorylation and activation of PLC-gamma 1 to produce Ins-P3 and then it mobilizes calcium from intracellular stores. This signal transduction pathway may play a role in regulating the proliferation of erythroid cells.

Calcium↗
PubMed1993

[Pathomorphologic changes on 150 chronic atrophic gastritis patients by treatment based on syndrome differentiation].

Xiang-sha Warming (XSW) and Shashen Nourishing Decoction (SSN) were used in treating Yang Deficiency Syndrome (YaDS) and Yin Deficiency Syndrome (YiDS) of chronic atrophic gastritis (CAG) respectively. 121 cases with Spleen YaDS, and 29 cases with Spleen YiDS were selected. 26 and 30 specimens were taken from gastric mucosa for observation under the SEM and TEM, including 12 and 12 YaDS cases, 14 and 12 Spleen Qi Deficiency cases respectively, 2 cases of YiDS were also observed with TEM. The specimens were taken from the same site of the gastric mucosa directly under the gastroscope pre- and post-treatment. 43 patients (35.6%) with spleen YaDS and 5 cases (17.2%) in Spleen YiDS showed marked effect after 3-month treatment. It showed that the effect in Spleen YaDS was better than YiDS. The XSW was superior than that of SSN. The effects were related to the syndromes and the degrees of pathologic change. This showed that the recipe could somewhat reverse and restore the abnormal glands of gastric mucosa.

Biopsy↗
PubMed1992

[Histopathology of spleen deficiency syndrome of chronic atrophic gastritis and its ultrastructural changes].

Pathologic change of gastric mucosa in 247 CAG patients of Spleen Qi (Yang) Deficiency (QYD) and Spleen-Stomach Yin Deficiency(YD) Syndrome were observed. The surface of gastric antrum mucosa of 26 patients with scanning electron microscope as well as the gastric mucosa of 34 patients with transmission electron microscope was observed for their intracellular structure. It was found that the CSAG and CAG I of Spleen QYD amounted to 165 cases (66.8%), and CAG II and III of Spleen-Stomach YD amounted to 34 cases (64.2%). It denoted that the Spleen QYD belonged to mild case, while the Spleen-Stomach YD belonged to severe case. The mitochondria of all kinds of gastric antrum mucosa cells showed retrograded degeneration, the pathologic basis of Spleen Deficiency of CAG, those of Spleen-Stomach QD had sparse broken and swollen mitochondria (82.3%), while the 53.3% of Spleen-Stomach YD displayed vacuolation as its predominant change. The different severity of retrograde degeneration and damage of mitochondria yielded various stage in atrophy of gastric mucosa which was the basis of Syndromes evolution from Defi. to QYD and then YD. Inflammatory change of the gastric mucosa was predominant in Spleen-Stomach YD (45.3%), and far less in QYD (27.1%), P < 0.01.

Gastric Mucosa↗