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H Zorn

Publications and source records attributed to H Zorn.

At least 19 recordsLinked to original sources
PubMed1979

[Simple method for the preparation of haptoglobin].

The present paper deals with a method permitting the isolation of haptoglobin 2-2 from human serum or plasma. The haptoglobin is adsorbed to DEAE-cellulose at pH 5.1 by batching. The loaded cellulose is given into a column and the haptoglobin eluted by a 0.1 M to 0.15 M acetate buffer. The last step is a gel-chromatography on Sephadex G-200. Disc- and immunoelectrophoresis were used to test the purity. As by-product acid alpha1-glycoprotein can be obtained.

Blood Proteins↗
PubMed1978

Proton NMR relaxation study of the binding of pyridine and 1-methylimidazole to some ferriporphyrins and to metmyoglobin.

Proton spin-lattice relaxation times of pyridine and 1-methylimidazole complexed on Fe(III)protoporphyrin IX dimethyl ester, Fe(III)tetraphenylporphyrin in chloroform and on metmyoglobin in 2H2O have been measured. Caused by chemical exchange of the ligand molecules into the bulk solvent phase, the decay of their MZ-magnetization is given by a superposition of two exponentials from which the mean lifetime of the complexed species can be determined. This method offers the possibility to study exchange kinetics of ligand molecules from a well defined molecular configuration. The present data are compared with the results from linewidth measurements of the bulk phase molecules. From both sets of parameters a detailed picture of the ligand exchange can be gained, particularly if spin transition of the paramagnetic organic metal complex occurs, as is the case for some ferriporphyrins. For metmyoglobin, the NMR result is compared with relaxation times extracted from temperature jump experiments under similar conditions.

Calorimetry↗
PubMed1975

Proton nuclear-magnetic-resonance study of low-spin ferriprotoporphyrin(IX) dimethyl ester.

Proton nuclear magnetic resonance shifts, spin-lattice and spin-spin relaxation times have been measured of low-spin bis-pyridine ferriprotoporphyrin(IX) dimethyl ester in chloroform. From the relaxation behavior the hyperfine coupling constant has been obtained and the contact term of the chemical shift was calculated. Deviations between measured and calculated chemical shifts may be attributed to second-order Zeeman interactions. The geometry of pyridine coordinated to the fifth and sixth position of ferriprotoporphyrin(IX) dimethyl ester was estimated from measured relaxation rates. From the non-exponential decay of the Mz magnetization a mean lifetime of taub = 50 ms for pyridine attached to low-spin ferriprotoporphyrin(IX) dimethyl ester was found at 253 K.

Electron Spin Resonance Spectroscopy↗