PubMed Health⌕ Search

Biomedical subjects

H Zuo

Publications and source records attributed to H Zuo.

At least 19 recordsLinked to original sources
PubMed2006

A review of 227 cases of small papillary thyroid carcinoma.

AIMS: To review differences in biological aggressiveness, clinical behaviors or selected surgical treatments between the PMC and the slightly larger PTC of 1.0<T<or=2.0 cm. METHODS: Two hundred and twenty-seven cases of papillary thyroid carcinoma not larger than 2.0 cm, diagnosed and treated at the Kuma Hospital, Kobe, Japan, with a 10-year follow-up from 1992 to 2003, were reviewed. RESULTS: The small PTCs demonstrated excellent outcomes, and persistent/recurrent disease was identified in only nine patients (4%). None of the patients died of the disease. A multivariate analysis revealed that massive extrathyroidal extension at presentation was the only independent prognostic factor for locoregional recurrence. Subdivision into PMCs and slightly larger tumors (1<T<or=2 cm) did not affect the excellent outcomes, but the patients in the latter subgroup received more lymph node surgery and displayed more aggressive clinico-histological features such as higher rates of extrathyroidal extension, lymph node metastasis, loss of polarity, invasive growth pattern and loss of cohesiveness. CONCLUSIONS: Small PTC not larger than 2.0 cm could be considered as favorable a prognosis as PMC.

Adenocarcinoma, Papillary↗
PubMed2001

Agro-climatic classification systems for estimating the global distribution of livestock numbers and commodities.

Investment in agricultural research in developing countries is being increasingly targeted at those agro-climatic zones and issues where the economic and environmental benefits may be expected to be greatest. This first requires that the zones themselves be defined, along with information on domestic livestock numbers and commodity output within agro-climatic zones in different countries. Different methods for classifying agro-climatic zones were compared. These included methods based on estimated length of growing period (LGP) using rainfall and temperature data, the ratio of precipitation to potential evapotranspiration (PET), and on more detailed agronomic models, remote sensing data and land use information. Zonation based on LGP has already been linked to existing national livestock data. By defining agro-climatic zones and relating concentrations of livestock populations to those of humans, it is possible to make realistic estimates of livestock populations and the production of livestock commodities for most developing countries. Detailed agro-climatic analyses of Mainland East Asia and Sri Lanka have recently been undertaken using the GROWEST agronomic model. Using this model as the basis of agro-climatic classification appears to be significantly superior, particularly in temperate environments, to approaches based solely on LGP. Different ways of subdividing countries and continents into agro-climatic or agro-ecological zones (AEZs) are reviewed in this paper. In addition, we show how the numbers of production and commodities from domestic livestock can be allocated to such zones. We also indicate how some of this information can be applied.

Agriculture↗
PubMed2001

[Complications of third ventriculostomy for hydrocephalus].

OBJECTIVE: To explore the reason of complications of third ventriculostomy and the method for reducing complications. METHODS: Fifteen third ventriculostomies and the related complications were analyzed retrospectively. RESULTS: Two patients had ventricular infection, one subdural fluid collection, one obstraction of fenestration site by neoplasm, one delayed herniation due to hematoma-occupied third ventricle. The complications were due to the inferior quality of the equipment and poor experience. CONCLUSIONS: Advanced equipment and understanding of possible complications can reduce complications.

Adolescent↗
PubMed2001

[The effect of lip repair on maxillary growth in patients with complete unilateral cleft lip and(or) palate].

OBJECTIVE: To investigate the different effects of lip repair on maxillary growth among patients with cleft lip and(or) palate, and also the possible mechanisms accounting for that differences. METHODS: The lateral cephalometric analysis was applied to 84 Chinese patients with unilateral cleft lip and(or) palate at the early stage of permanent dentition, which included 32 UCLA, 20 UCLP (CP unrepaired), and 32 UCLP, and 37 normal Chinese of the same age as controls. RESULTS: The patients with unilateral cleft lip and palate who had lip repaired while the palate was left unrepaired exhibited the similar inhibition on maxillary growth to those who had both lip and palate repaired but the patients with unilateral cleft lip and alveolus had much less inhibition on their maxilla. CONCLUSIONS: The cleft and tissue defect of patients with cleft lip and palate might be the main cause that induced the inhibitive effects of lip repair on maxillary growth.

Cleft Lip↗
PubMed2001

[Relationship between the expression of Bax and apoptosis induced by hyperthermia in BcaCD885 cells].

OBJECTIVE: This study aimed to investigate the role of Bax gene in apoptosis induced by hyperthermia in BcaCD885 cells. METHODS: After inducing apoptosis through hyperthermia, the expression of Bax protein and mRNA were examined with FCM antibody analysis and RT-PCR technique respectively. RESULTS: Up regulation of Bax protein was noted after hyperthermia. The correlation coefficient r between the apoptotic rate and the expression Bax protein was 0.9240. The expression of Bax mRNA was also raised after hyperthermia. CONCLUSION: Hyperthermia acting as an external signal may up-regulates expression of Bax gene and then induces apoptosis in BcaCD885 cells.

Apoptosis↗
PubMed2001

[Inhibitive effects of lip repair on maxillary growth in patients with complete unilateral cleft lip and palate].

OBJECTIVE: The aim of this study was to assess the isolated effects of lip repair on inhibition of maxillary growth in patients with complete unilateral cleft lip and palate. METHODS: The lateral cephalometric analysis were applied to 20 patients with unilateral cleft lip and palate who only had lip repaired in childhood, 32 patients with unilateral cleft lip and palate who had both lip and palate repaired in childhood, as well as 37 normal Chinese of the same age as controls. RESULTS: Both complete unilateral cleft lip and palate groups had almost the same significant degree of maxillary retrusion compared with the normal control group. CONCLUSION: Lip repair is one of important factors that could inhibit maxillary growth in patients with complete unilateral cleft lip and palate.

Adolescent↗
PubMed2000

Development of surgical confocal scanning microscope for intra-operative imaging of brain tumors using near infrared fluorescence: technical note.

We developed a confocal laser scanning microscope for intra-operative imaging of brain tumors using near infrared fluorescence. The quality of near infrared images of Indocyanine Green (ICG) was compared with the surgical confocal scanning (SCS) microscope and a conventional charge-coupled device (CCD) camera; we compared images of a tube filled with ICG, which was located in the mouse brain. Compared to the CCD camera, the SCS microscope could obtain a more precise image of ICG fluorescence through the brain tissue. In addition, the SCS microscope could image ICG fluorescence clearly in a relatively light room because of elimination of stray light, while the CCD camera required high darkness to obtain ICG images. The present SCS microscope can give useful intra-operative imaging of brain tumors, particularly detection of residual tumor tissues that extend into normal brain tissues.

Animals↗
PubMed2000

[Long-term follow-up results of microsurgical treatment for glossopharyngeal neuralgia].

OBJECTIVE: To discuss effective surgical methods for the treatment of glossopharyngeal neuralgia. METHODS: During 1986 - 1998, 21 patients with glossopharyngeal neuralgia were treated microsurgically. Of whom, 5 patients were treated by microvascular decompression (MVD), 4 by MVD combined with rhizotomy of glossopharyngeal nerve root and upper 1st to 2nd rootlets of vagus nerve root, and 12 by rhizotomy of glossopharyngeal nerve root and upper 1st to 2nd rootlets of vagus nerve root. RESULTS: Neuralgia disappeared in all patients postoperatively. In 3 patients, occasional coughing episodes occurred postoperatively. One of the 3 patients also suffered from mild hoarseness and swallowing difficulty. All patients were followed up on average 7.2 years with no recurrence. CONCLUSIONS: MVD, rhizotomy of glossopharyngeal nerve root and upper 1st to 2nd rootlets of vagus nerve and their combination are effective for the treatment of glossopharyngeal neuralgia. Operative methods should be chosen according to the following two points: compression of nerve roots by affected vessels or not; and the condition of compression.

Adult↗
PubMed2000

[Identification of telomerase activity in the experimental carcinogenesis of oral cavity].

OBJECTIVE: Telomerase is a ribonucleoprotein enzyme which synthesizes telomere DNA repeat sequences and maintain stably telomere length. The activity of telomerase may be necessary for the growth of immortalized cells overcoming cellular senescence. The researches have shown that telomerase activities are associated with most cancers. The purpose of the study is to detect the development of telomerase activity during golden hamster cheek pouch carcinogenesis induced by DMBA. METHODS: First, 52 golden hamsters were divided into 2 groups. Four of them were not done any special treatment and were killed after 3 days. The others were covered with DMBA on the surface of cheek pouch on one side in order to induce carcinogenesis, the other side of cheek pouch was treated as the control. Then, the 48 golden hamsters were divided into 4 groups and were killed in 7, 10, 14 and 20 weeks. The telomeric repeat amplification protocol (TRAP) based on PCR and ELISA was used to analysis the activity of telomerase. RESULTS: 1. The expression of telomerase activity existed in normal cheek pouch mucosa of golden hamsters, which meant that telomerase played an important role on controlling cell proliferation. 2. The level of telomerase activity gradually increased while hyperplasia and dysplasia was observed in the cheek pouch mucosa covered with DMBA. It reached its top at a later premalignant period and gradually decreased after that. 3. There was negative correlation between the degree the telomere length was shortened and the activity of telomerase (r = -0.9654), and there was positive correlation between the reduced rate of telomere length and the activity of telomerase (r = 0.9471). CONCLUSION: The activity of telomerase is one of important factors that can effect the stability of telomere length and plays a crucial role in the progression of oral cancer. So the activity of telomerase is an early molecular marker of carcinogenesis.

Animals↗
PubMed2000

[The method of time series modeling and its application in the spectral analysis of lubricating oil].

In this paper, we discuss the applications of time series modeling method in the analysis of lubricating oil of mechanical equipment. We obtained satisfactory results by applying AR model to perform time series modeling and forecasting analysis to the collected spectral analysis data of the air engine. So we have built a practical method for state monitoring and trouble forecasting of mechanical equipment.

English Abstract↗
PubMed1999

Cerebral blood oxygenation changes induced by auditory stimulation in newborn infants measured by near infrared spectroscopy.

Recent neuronal activation studies on newborns using functional MRI or near infrared spectroscopy (NIRS) have suggested that the increase in O2 consumption accompanying neuronal activation exceeds the increase in O2 delivery in the visual cortex during photic stimulation. In the present study, we evaluated the cerebral blood oxygenation (CBO) changes induced by auditory stimulation in the frontal lobe of newborns using NIRS. We studied 28 newborns; the postnatal age at CBO measurements was 3.1+/-0.3 days (mean+/-S.E.M.). We measured concentration changes of deoxyhemoglobin (Deoxy-Hb), oxyhemoglobin (Oxy-Hb), and total hemoglobin (Total-Hb) induced by auditory (music) stimulation in the bilateral frontal lobes of the newborns. Twenty-six (92.9%) out of 28 subjects showed increases of Oxy-Hb and Total-Hb during the stimulation. In these subjects, 17 (60.7%) subjects showed an increase of Deoxy-Hb associated with increases of Oxy-Hb and Total-Hb, while nine (32.1%) subjects showed a decrease of Deoxy-Hb. Although the direction of the Deoxy-Hb differed, these two groups did not differ for Oxy-Hb and Total-Hb (P > 0.05). Two (7.1%) subjects showed other changes. The frontal lobe of newborns shows CBO responses similar to those observed in the visual cortex, specifically neuronal activation causes an increase of Deoxy-Hb associated with increases of Oxy-Hb and Total-Hb. These results support the hypothesis that increments in O2 consumption exceed increments in O2 delivery during neuronal activity in newborns.

Acoustic Stimulation↗
PubMed1998

Continuous suction surgical cottonoid for microneurosurgery.

A new suction device for microneurosurgery is presented. A surgical cottonoid is covered with a polyethylene film so that vacuum power is maintained throughout the length of the cottonoid. One end is connected to a vacuum pump via a plastic tube. Five to 10 mm of the surgical cottonoid are exposed for suctioning at the other end of the cottonoid. The suction surgical cottonoid is flexible and safe, avoiding tissue damage. In addition, the surgeon can manipulate the exposed cottonoid at the tip as any other surgical cottonoid.

Equipment Design↗
PubMed1998

Language-activated cerebral blood oxygenation and hemodynamic changes of the left prefrontal cortex in poststroke aphasic patients: a near-infrared spectroscopy study.

BACKGROUND AND PURPOSE: In normal subjects, regional cerebral blood flow (rCBF) is greatly increased by neuronal activity, whereas the cerebral metabolic rate for O2 is increased only slightly. However, it is not clear what kinds of cerebral blood oxygenation and hemodynamic changes can be induced by language activities in language-relevant areas of poststroke aphasics. In the present study, we investigated the difference in the changes of cerebral blood oxygenation and hemodynamics in the left prefrontal cortex induced by language activities between normal subjects, poststroke nonaphasic patients, and nonfluent aphasic patients using near-infrared spectroscopy (NIRS). METHODS: Twenty-nine participants performed speech tasks, such as confrontational naming, to evaluate changes among poststroke nonfluent (Broca's) aphasia patients (10 cases; mean+/-SEM, 56.9+/-2.2 years), age-matched normal subjects (13 cases; 50.7+/-2.2 years) and poststroke nonaphasic patients (6 cases; 52.5+/-3.9 years). The optodes of NIRS were placed over the left prefrontal cortex. We analyzed the NIRS parameter (oxyhemoglobin [oxy-Hb], deoxyhemoglobin [deoxy-Hb], and total hemoglobin [total-Hb]) changes by qualitative pattern analysis of the parameter changes and quantitative analysis of the parameter values among the groups. RESULTS: The most common NIRS parameter change was an increase in oxy-Hb and total-Hb, with a slight decrease or no change in deoxy-Hb in the normal subjects (5 of 13 cases, 38.5%) and the nonaphasic cerebrovascular disease (CVD) patients (3 of 6 cases, 50.0%). In contrast, the most common pattern in the aphasic patients was an increase of deoxy-Hb, with an increase of oxy-Hb and total-Hb (5 of 10 cases, 50%). However, this pattern was observed in only 3 of 13 cases (23.1%) in the normal subjects and 1 of 6 cases (16.7%) in the nonaphasic CVD patients. The mean (+/-SEM) changes of deoxy-Hb of the aphasic patients, the normal subjects, and the nonaphasic CVD patients were 0.78+/-0.29, 0.06+/-0.16, and -0.18 +/- 0.22, respectively. The statistical analysis demonstrated a significant effect for deoxy-Hb (P<0.05), with the aphasic patients differing significantly from the normal subjects and the nonaphasic CVD patients, while the 2 nonaphasic groups did not differ from each other. CONCLUSIONS: The present results demonstrate a multiplicity of language-activated cerebral blood oxygenation and hemodynamic changes in the left prefrontal cortex in the nonaphasic and aphasic groups. The increase of deoxy-Hb with increases of oxy-Hb and total-Hb in the aphasics during language tasks suggests that the left prefrontal cortex of the aphasics utilizes more oxygen than the nonaphasics during language tasks. Finally, functional MRI, which images the activation area in the brain by detecting the reduced concentration of deoxy-Hb during neuronal activation, should be performed on the patients with cerebral dysfunction, giving special consideration to the possible multiplicity of the rCBF and cerebral oxygen metabolism responses to functional tasks.

Aged↗
PubMed1998

[Evaluation of the effectiveness nine years after primary immunization with local produced plasma-derived hepatitis B vaccine].

OBJECTIVE: To observe the effectiveness of hepatitis B vaccine nine years after primary immunization. METHODS: A randomized, controlled and double-blinded trial was designed with 126 children primarily immunized with local produced plasma-derived hepatitis B vaccine nine years ago and 135 placebo-control children aged five to nine years. All the children were followed-up, their blood samples collected and their disease occurrence examined. Their hepatitis B surface antigen (HBsAg), antibody against hepatitis B core antigen (anti-HBc) and antibody against hepatitis B surface antigen (anti-HBs) were tested. Causes of lowering in their antibodies were analyzed with multivariate methods. RESULTS: During the nine-year follow-up, 4% to 19% of the study subjects dropped out. In the ninth year after immunization (T108), anti-HBs positive rate in the vaccinees and their geometric mean titer (GMT) (65.3% and 5.47, respectively) were still significantly higher than those in the placebo-controls (28.3% and 1.46, respectively), but significantly lower than those in the 6th year after immunization (T72). Multivariate analysis for the causes of lowering in the antibody during the 4th, 6th and 9th year after immunization showed that antibody titer in the vaccines first year after immunization (T12) was a crucial factor for the persistence of the antibody, and natural booster might also played a certain role in it. In the T108, one clinical case of hepatitis B occurred in the placebo control group, but no cases in the vaccinees found, with a protection rate of 81.4% from HBV infection and 84.6% from HBsAg positive-coversion, similar to those in the T72. CONCLUSION: The effectiveness of HB vaccine still existed nine years after primary immunization, and no booster dose was needed so far.

Child↗
PubMed1997

Noninvasive optical imaging of the subarachnoid space and cerebrospinal fluid pathways based on near-infrared fluorescence.

The authors have developed a noninvasive optical method to image the subarachnoid space and cerebrospinal fluid pathways in vivo based on the near-infrared fluorescence of indocyanine green (ICG). The ICG was bound to purified lipoproteins (ICG-lipoprotein) and injected into the subarachnoid space of neonatal and adult rats. The ICG fluorescence was detected by a cooled charge-coupled device camera. After injection of ICG-lipoprotein into the cerebral subarachnoid space of the neonatal rat, ICG fluorescence was clearly detected at the injection site through the skull and skin. The ICG fluorescence was observed in the cerebellum and the lumbar spinal cord 1 and 8 hours postinjection, respectively. After injection of ICG-lipoprotein into the lumbar spinal subarachnoid space of an adult rat, ICG fluorescence was observed from the injection site to the thoracic levels along the spinal subarachnoid space. In addition, with the rat's head tilted downward, ICG fluorescence had extended to the cerebral subarachnoid space by 1 hour postinjection. The ICG fluorescence imaging of the cerebral subarachnoid space demonstrated an increase in fluorescence intensity around the lambdoid suture and the forebrain. On dissection of the rat brain the former location was identified as the supracerebellar cistern and the latter as the olfactory cistern. The results of this study are the first to demonstrate that an optical technique is applicable to imaging of the subarachnoid space and cerebrospinal fluid pathways in vivo. In addition, ICG-lipoprotein provides a sensitive optical tracer for imaging extravascular biological structures. Finally, ICG fluorescence imaging does not require an intricate imaging system because ICG is localized near the surface of the body.

Animals↗
PubMed1996

[Transsphenoidal microsurgery for pituitary adenoma: analysis of 3 fatal cases].

To prevent severe postoperative complications and increase the survival rate of patients with pituitary adenoma after transsphenoidal microsurgery, 3 fatal cases were analysed in terms of clinical features and caused of death. They included (1) the too deeply inserted suction apparatus with resultant midbrain damage; (2) bleeding from residual tumor into aphragma sellae and dura mater, entering the subarachnoid, causing spasm of the brain vessels. In order to avoid complication, it is necessary to follow strictly the rules for transsphenoidal microsurgery and improve surgical skills.

Adenoma↗
PubMed1995

Determination of alpha-difluoromethylornithine in blood by microdialysis sampling and capillary electrophoresis with UV detection.

A procedure is described for the analysis of alpha-difluoromethylornithine (DFMO), an anti-cancer agent, in plasma microdialysis (MD) samples. DFMO has been shown to be effective alone or in combination with other agents in the treatment of several cancers. Precolumn derivatization of DFMO with naphthalene-2,3-dicarboxaldehyde-cyanide (NDA-CN) in pH 10.0 borate buffer results in the rapid formation of a stable mono-derivatized product (N-substituted 1-cyanobenz[f]isoindole, CBI), which is UV active. An analytical method has been developed to separate CBI-DFMO from NDA-CN derivatization products of 20 standard amino acids using capillary electrophoresis (CE). This method is then employed for the determination of DFMO in plasma microdialysis samples. Separation of DFMO from other components in the dialysate was achieved within 20 min. The response for DFMO in Ringer's solution was linear over the range of 1.2 x 10(-6) to 1.6 x 10(-4) M after derivatization. The detection limit of DFMO in the plasma dialysate is 5 microM using UV detection at 254 nm. This method has been proven to have adequate sensitivity for quantitation of DFMO in i.v. microdialysate samples and has been successfully applied to monitoring the pharmacokinetics of DFMO by CE-UV.

Animals↗