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Hajime Utsuno

Publications and source records attributed to Hajime Utsuno.

3 recordsLinked to original sources

Facial soft tissue thickness in Japanese female children.

Facial reconstruction techniques used in forensic anthropology are based on soft tissue thickness measurements. Many studies of facial tissue thickness in adults have been published that take racial background into account. However, the only data on facial thickness in children are derived from studies of American, British, and Hispanic children. The authors therefore measured facial tissue thickness in Japanese children, with the aim of providing data for producing accurate facial likenesses and to evaluate matching of skull-photo superimposition images. Cephalometric X-ray images give an approximately 10% enlargement from true size and can demonstrate the relationship between soft and hard tissue. Facial soft tissue thickness was measured at 12 anthropological points using X-ray cephalometry.

Adipose Tissue↗

Preliminary study of post mortem identification using lip prints.

Identification using lip prints was first performed in the 1950s and was the subject of much research in the 1960s and 70s, leading to the acceptance of this technique as evidence in the criminal justice system. Previous research has focused on identifying lip print types or on methods of obtaining hidden lip prints left at the crime scene. The present study aimed to clarify characteristics of lip prints from cadavers with various causes of death (including drowning and hanging) and to determine the effects of fixation on post mortem changes in lip impressions.

Adult↗

Influence of template DNA degradation on the genotyping of SNPs and STR polymorphisms from forensic materials by PCR.

Detection of single nucleotide polymorphisms (SNPs) and short tandem repeat (STR) polymorphisms by PCR is widely used to analyze degraded DNAs in forensic science. The success of DNA analysis from human remains largely depends on the quality of the template DNA. We examined two SNPs (HLA-DQA1 and ABO) and two STR polymorphisms (VWA and CD4) by SSCP gel or denaturing gel electrophoresis, using two kinds of degraded DNA samples (165 teeth and blood stains contaminated with saliva) derived from the same person and investigated the influence of template DNA degradation on genotyping. As the degradation of DNA proceeds, unbalanced amplification of alleles occurred in the analysis of both SNPs and STRs, followed by allele drop, and further by loss of amplification. Non-target allelic products of STRs were amplified from highly degraded DNA samples; however, false allelic products of SNPs were not amplified from them. Amplification efficiency increased in proportion to the decrease of PCR target size, but reduction of the PCR target sizes also increased the chances of amplifying contaminating DNA, especially in highly degraded DNA specimens. The present results will help investigators to evaluate the genotyping of highly degraded DNA samples in forensic casework.

ABO Blood-Group System↗