Biomedical subjects

Hao Yang

Publications and source records attributed to Hao Yang.

27 records · Page 2Linked to original sources

PubMed2004

[Study of correlation dimension on EEG].

The study of non-linear EEG is of great significance in clinical practice and research work. This paper has gone into the feasibility of calculating the correlation dimension and has developed some subjects with the characters of correlation dimension and the difference under four conditions: (1) passive eyes closed(PEC); (2) mental arithmetic with eyes closed(MAEC); (3) passive eyes open(PEO); (4) mental reasoning with eyes open (MRED). The results show it is feasible and meaningful to calculate correlation dimension and the correlation dimension can reflect the regular patterns of mental activity.

Algorithms↗

PubMed2004

[Recovery of CO from an ammonia plant tail gas with pressure swing adsorption process].

Ammonia plant tail gas is an important CO source for C1 chemistry if an efficient separation/purification technology is available. Presented is pressure swing adsorption using a home made catalyst PU1 for recovery of CO from ammonia plant tail gas. A performance comparison of PU1 with a commercial zeolite was also presented. The pilot plant experiments were carried out in an intermission one-bed PSA unit with ammonia plant tail gas and a feed flow rate of 0.2-0.8 m3/h. The purity and recovery of CO was studied under various operating conditions. The operating conditions for the process were optimized and an optimum cyclic sequence of operation steps was proposed. A good performance of PU1 in the process was experimentally demonstrated. Product gas with a CO purity over 98% could be produced at a CO recovery of 75% for a feed gas CO concentration of 30% and gas pressure of 0.3 MPa.

Adsorption↗

PubMed2003

Differentiation-inducing and protective effects of adult rat olfactory ensheathing cell conditioned medium on PC12 cells.

Transplantation of olfactory ensheathing cells has been one of the promising strategies in enhancing central nerve fiber regeneration. Membrane surface molecules on olfactory ensheathing cells mediating cell-cell interactions as well as various factors released from them are thought to be important for nerve regeneration. The latter, however, has not been fully substantiated by experimental data, particularly regarding the olfactory ensheathing cells of the adult animals. In the present study, the effects of adult olfactory ensheathing cell conditioned medium on PC12 cells were examined. The results show that the factors secreted by the adult olfactory ensheathing cells can promote PC12 cell differentiation and protect it from Zn(2+) insult.

Animals↗

PubMed2003

[Noninvasive monitoring of hypertensive intracerebral hemorrhage and perihematomal tissue edema].

OBJECTIVE: To investigate the dynamic change regularity of intracerebral hemorrhage and perihematomal tissue edema. METHODS: The cerebral electric impedance (CEI) of 100 normal volunteers and 24 patients with hypertensive intracerebral hemorrhage was measured, and the results of measurement were compared with the volumes of hematoma detected by CT. RESULTS: In the normal persons, the CEI values in the left and right sides of cerebral hemispheres were 11.5 +/- 1.3 k(Omega) vs 11.4 +/- 1.8 k(Omega) at the frequency of 20 kHz, 10.2 +/- 1.0 k(Omega) vs 10.3 +/- 1.1 k(Omega) at the frequency of 50 kHz, and 9.1 +/- 0.8 k(Omega) vs 9.2 +/- 0.8 k(Omega) at the frequency of 100 kHz (all P > 0.05). Age, sex and measuring time did not obviously affect the CEI values. In the patients with hypertensive intracerebral hemorrhage the CEI of hematoma side was significantly decreased within 2 days after onset and then increased significantly for more than 10 days. The rate of EI change in the hematoma side within 24 h had a negative correlation with the volume of hematoma (r = -0.895 3, P < 0.01). however, the rate of CEI change in the hematoma side had a positive correlation with the volume of perihematomal edema (r = 0.881 1, P < 0.01). CONCLUSION: CEI is a stable physical parameter reflecting the electrical character of human brain tissue. CEI sensitively reflects the change of hematoma and perihematomal edema, and guides the clinical treatment of cerebral hemorrhage.

Adolescent↗

PubMed2003

Intraneuronal localization of Nogo-A in the rat.

Nogo-A is known to be a myelin-associated protein with strong inhibitory effect on neurite outgrowth and has been considered one of the major factors that hinder fiber regeneration in the central nervous system. Recent studies have demonstrated widespread occurrence of nogo-A mRNA and Nogo-A protein in neurons. Our concurrent immunohistochemical study substantiated the widespread distribution of neuronal Nogo-A. The present study was thus focused on its intraneuronal distribution in the central nervous system, using Western blotting, immunohistochemical, and immunogold electron microscopic techniques. Western blotting of the nucleus, cytoplasm, and membrane subcellular fractions of the cerebellum and spinal cord tissues demonstrated that all three fractions contained Nogo-A. Nogo-A immunoreactivity could be identified under confocal microscope in the nucleus, perikayon, and proximal dendrite and along the cell membrane. Under the electron microscope, the perikaryonal Nogo-A immunogold particles were mainly distributed at polyribosomes and rough endoplasmic reticulum, suggesting its relationship with translation process. The immunogold particles could also be found beneath or on the plasma membrane. In the nucleus, the Nogo-A immunogold particles were found to be localized at the chromatins of the nucleus, indicating its possible involvement in gene transcription. The presence of Nogo-A in the nucleus was further supported by transfection of COS-7L cells with nogo-A. This study provides the first immunocytochemical evidence for intraneuronal distribution of Nogo-A. Apparently, the significance of Nogo-A in the central nervous system is far more complex than what has been envisioned.

Animals↗

PubMed2003

Effects of valsartan with or without benazepril on blood pressure, angiotensin II, and endoxin in patients with essential hypertension.

AIM: To evaluate the effects of valsartan (Val) with or without benazepril (Ben) on blood pressure and plasma levels of angiotensin (Ang II) and digoxin-immunoreactive factors (endoxin) in patients with essential hypertension. METHODS: Ninety patients with essential hypertension were randomly divided into 3 groups (n=30 per group): Ben group (Ben 10 mg/d, po); Val group (Val 80 mg/d, po); combination drug therapy group (Val 80 mg/d+Ben 10 mg/d, po); all patients were treated for 12 weeks. Age and sex-matched 20 normal subjects were served as control group. RESULTS: The levels of plasma endoxin and Ang II in patients with essential hypertension were remarkably higher than those in normal subjects. The levels of plasma Ang II and endoxin were all obvious positive correlation with systolic blood pressure (SBP) and diastolic blood pressure (DBP) (Ang II: r=0.5151, 0.7978; endoxin: r=0.4706, 0.7274, respectively). Within 6 weeks of drug intervene, SBP and DBP were remarkably decreased in 3 groups. After 6 weeks, SBP and DBP were continuously decreased in Ben group and Val+Ben group, but not in Val group. Level of plasma Ang II was remarkably decreased as SBP and DBP decreased in Ben group and Val+Ben group; level of plasma Ang II was remarkably increased in Val group. CONCLUSION: Val with or without Ben remarkably decreased SBP and DBP in patients with essential hypertension within 6 weeks. Antihypertensive efficacy was weakened after long-term use of Val alone. The antihypertensive effect of Val+Ben group was the most remarkable among 3 groups and could avoid the side effects of high plasma Ang II.

Adult↗

PubMed2003

[Expression and biological activity of human neurotrophin-3 in olfactory ensheathing cells mediated by retroviral vector].

We successfully constructed a retrovirus expression vector pRev-TRE-NT3. The supernatant with highest retroviral titers was obtained by transfection of two retrovirus vector, pRev-TRE-NT3 and pRev-Tet-On, into an ecotropic Ecopack-293 cells. Primarily cultured rat olfactory ensheathing cells (OECs) were co-infected with retrovirus following pRev-TRE-NT-3 and pRev-Tet-On system of produced retrovirus. By adding different concentrations of Doxycline, genetically modified OECs were induced to secrete human NT-3. The secreted NT-3 in OEC culture supernatant was detected with western-blot, and its biological activity was confirmed by the growth bioassay of dorsal root ganglion (DRG) cells. The results are as follows: (1) 0.78 kb hNT-3 cDNA was harvested and successfully cloned into pRev-TRE vector by PCR and restriction enzyme digestion methods. The constructed plasmid was identified with electrophoresis, and the direction of hNT-3 cDNA inserting pRev-TRE vector was integrated correctly; (2) NT-3-modified OEC culture supernatant contained 28 kd NT-3 which can bind NT-3 antibody in western-blotting assay, and NT-3 expression was concentration-dependent on Doxycline. NT-3 was not detected in control groups; and (3) Numerous DRG cells migrated from DRG tissue mass in NT-3-modified OECs experimental groups, and these cells had long and thin processes with strong dioptre. These processes formed complicated networks. As for unmodified OEC control groups, a few DRG cells migrated from the tissue mass, and the processes of these cells were rather short. In blank groups, no cells migrated and grew. And their processes in control groups are significantly shorter than NT-3-modified OECs experimental groups. Our results indicate that Tet-On-regulated NT-3 expression in OECs was efficient, offering novel material of in vivo transplantation for the repair of CNS injury.

Animals↗

PubMed2003

[A simulation study on brain hematoma real time monitoring by use of electrical impedance tomography].

It is significant to develop a new set of medical equipment in which electrical impedance tomography is applied to brain hematoma real time monitor. This paper describes some progress in reconstruction algorithm of EIT. The methods make it possible to improve the precision as well as computation speed dramatically. In addition, intensive simulation describes all the process for real time monitoring of brain hematoma by exploiting EIT.

Algorithms↗

PubMed2003

[Physiotherapy with drugs for acute lymphadenitis of face and neck].

OBJECTIVE: To observe the clinical efficacy of acute lymphadenitis of face and neck treated by physiotherapy with drugs. METHODS: Ninety-six children with acute lymphodenitis of face and neck were randomly divided into 2 groups. Of the 96 patients, 48 were treated by drugs as the control group, and the other 48 were treated by physiotherapy with drugs as the treatment group. Improvement of clinical symptoms and signs was compared after 3 days, and the treatment period was observed. RESULTS: Clinical symptom and sign of the treatment group were improved more than those of the controls (P < 0.01); the treatment period of the treatment group was shorter than that of the controls (P < 0.01). CONCLUSION: It is more effictive and costs less time to treat acute lymphadenitis of face and neck with physiotherapy with drugs.

Acute Disease↗