A putative neuromuscular acetylcholinesterase gene from Dictyocaulus viviparus.
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Acetylcholinesterase↗
Biomedical subjects
Harm W Ploeger
Publications and source records attributed to Harm W Ploeger.
Path analysis of subsistence farmers' use of veterinary services in Zimbabwe.
We used factor and path analyses to identify the causal paths or relationships between variables affecting the demand and use by subsistence farmers of the various services offered by government-employed veterinary livestock technicians (VLTs) in Zimbabwe in 1996. We examined whether the farmer had implemented the VLT-recommended livestock-management procedures. Various factors that have been reported or theorized as important in the demand for veterinary services by subsistence farmers are described. The important factors positively affecting whether farmers had sought veterinary assistance in the last 24 months included familiarity of the farmer with the VLT, if situated closer to the VLT's base, and if the farmer received further training in agriculture (either through courses or from attendance at demonstrations on animal husbandry conducted by the VLT). Factors affecting whether farmers implemented the recommended management practices that had a monetary cost included familiarity with the VLT, proximity to the VLT's premises, farmer literacy, club membership, and farmer's wealth. There was a difference in the paths generated for preventive measures that had a monetary cost versus those measures that involved only a labour or effort costs. For the former practices, the VLT played an important role informing farmers by extension and demonstrations, and farmer's membership of a club increased their performance.
Animal Husbandry↗
Cytochrome oxidase c subunit 1 polymorphisms show significant differences in distribution between a laboratory maintained population and a field isolate of Cooperia oncophora.
A 474 bp fragment of the mitochondrial cytochrome oxidase c subunit 1 (cox1) of Cooperia oncophora was cloned and sequenced. The overall nucleotide diversity of the cox1 fragment varied from 0.5 to 2.0% between individuals. Two nucleotide substitutions were found within two RsaI endonuclease restriction sites and were used in a PCR-based restriction fragment length polymorphism (PCR-RFLP) assay to asses the intra-population variation of C. oncophora. Testing 816 individuals revealed the existence of three different haplotypes, having either both (type I) or only one (types II and III) RsaI site. Laboratory maintained individuals obtained at different time points after infection showed no significant difference in the distribution of the three haplotypes. Neither was there a difference in the distribution between male and female worms, confirming that the mitochondrial genome of C. oncophora is also maternally inherited. Nevertheless, there was a significant difference in the prevalence of the RsaI point mutation in the cox1 gene between the laboratory maintained population of C. oncophora and a Dutch field isolate, indicating that these RFLPs can be used to study genetic variation within or among C. oncophora populations.
Animals↗
Dictyocaulus viviparus: re-emerging or never been away?
For over 40 years a highly effective vaccine against the bovine lungworm has been commercially available. The use of it successfully reduced the number of outbreaks in calves. However, the past decade has seen a dramatic increase in lungworm outbreaks in adult cows in the UK. This might indicate that Dictyocaulus viviparus is re-emerging as a significant parasite in the dairy cattle industry. Much is still unknown, and here the most important aspects requiring urgent attention are put into perspective.
Animals↗