Illegal import of equine wormers.
Explore the source record for details and available documents.
Animals↗
Biomedical subjects
Helen Barnes
Publications and source records attributed to Helen Barnes.
v-Src induces Shc binding to tyrosine 63 in the cytoplasmic domain of the LDL receptor-related protein 1.
We recently observed that the LDL receptor-related protein 1 (LRP-1) is tyrosine phosphorylated in v-Src-transformed cells. Using a GST-fusion protein containing the cytoplasmic domain of LRP-1, we show that LRP-1 is a direct substrate for v-Src in vitro. To study LRP-1 phosphorylation in vivo, we constructed an LRP-1 minireceptor composed of the beta chain linked at the amino-terminus to a Myc epitope (Myc-LRPbeta). When expressed together with v-Src, Myc-LRPbeta becomes phosphorylated on tyrosine. Of the four tyrosine residues present in the cytoplasmic domain of LRP-1, only Tyr 63 is phosphorylated by v-Src in vivo or in vitro. Using fibroblasts deficient in Src, Yes and Fyn, we were able to show that there are multiple kinases present in the cell that can phosphorylate LRP-1. Tyrosine-phosphorylated LRP-1 associates with Shc, a PTB and SH2 domain containing signaling protein that is involved in the activation of Ras. Binding of the purified Shc PTB domain to Tyr 63 containing peptides shows that the interaction between LRP-1 and Shc is direct. We found that DAB, a PTB domain containing signaling protein that is involved in signaling by LDL receptor-related proteins in the nervous system, did not bind to full-length LRP-1. Our observations suggest that LRP-1 may be involved in normal and malignant signal transduction through a direct interaction with Shc adaptor proteins.
Adaptor Proteins, Signal Transducing↗
Evaluating a respiratory intermediate care team.
This article examines the work of a respiratory intermediate care team. Findings from an audit and a patient satisfaction postal questionnaire are presented to assess the effectiveness of the team's work and evaluate the effect of specialist nursing teams on hospital bed days and patient preference. From the evaluation it is apparent that the respiratory intermediate care team is an effective means of saving hospital bed days through prevention of admission and early discharge in patients with chronic respiratory disease. The results of the patient satisfaction postal questionnaire show that the majority of patients find hospital-at-home style care favourable because they feel well-supported at home to self-manage their condition. This outcome is important because it indicates that specialist nursing teams in primary care are valuable to patients and save the NHS money.
Acute Disease↗