Biomedical subjects
Publications and source records attributed to Helfried Reuther.
The native extracellular matrix (ECM) laid down on silicon and titanium surfaces by osteoblast-like SAOS-2 cells was exposed by selective removal of cells. This type of material surface ECM-Si, ECM-Ti was shown to promote the nucleation of calcium phosphate from a simulated body fluid (SBF). Microscopic and spectroscopic results revealed the effect was associated with a collagen fiber-free extracellular matrix.
Osteoblast-like SAOS-2 cells were allowed to synthesize and assemble their extracellular matrix (ECM) on titanium surfaces. After the selective removal of cells, Ti coated with a native ECM was obtained (ECM-Ti). The responses of SAOS-2 cells to ECM-Ti compared with those to peptide sequence RGDS- or fibronectin-immobilized Ti were examined, demonstrating the compositional and structural effects needed to trigger the native cell behavior.
Interfacing of water vapor and titanium was employed for a controlled synthesis of surface-supported hydroxyapatite. In this approach, a Ti surface was doped with Ca and P by ion implantation and then subjected to a hydrothermal treatment in a water vapor autoclave. Ion implantation served to prepare a reactive blend of finely dispersed reactants in a stoichiometric ratio incorporated within the network of an outermost layer of the substrate surface. Needle-like carbonate hydroxyapatite in an overlay was identified to deposit onto the surface. The results suggest interface-mediated thin film formation and phase transformation.