Biomedical subjects

Hideaki Kano

Publications and source records attributed to Hideaki Kano.

4 recordsLinked to original sources

PubMed2006

Three-dimensional vibrational imaging of a microcrystalline J-aggregate using supercontinuum-based ultra-broadband multiplex coherent anti-stokes Raman scattering microscopy.

Vibrational properties of a porphyrin J-aggregate microcrystal have been investigated by ultra-broadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy using a supercontinuum light source generated from a photonic crystal fiber. Owing to a strong resonance effect due to an excitonic transition, clear spectral and spatial profiles of the CARS signal have been successfully obtained. On the basis of the comparison between the CARS and the fluorescence images, the spatial dependence of the CARS signal can be explained by the spatial inhomogeneity of the excitonic transition energy in the single J-aggregate microcrystal.

Journal Article↗

PubMed2006

Hyper-Raman microspectroscopy: a new approach to completing vibrational spectral and imaging information under a microscope.

We have developed hyper-Raman scattering microspectroscopy and applied it to a microcrystal of all-trans-beta-carotene. The hyper-Raman spectrum of all-trans-beta-carotene exhibits a Raman-inactive but infrared-active vibrational mode at 1564 cm(-1). Hyper-Raman imaging of a microcrystal was performed with this band. Infrared-active vibrational imaging was achieved with a spatial resolution much higher than that of conventional infrared microscopy. The combination of Raman and hyper-Raman spectroscopy opens up a new scope for high-spatial-resolution vibrational microspectroscopy that is not restricted by the selection rule.

Crystallography↗

PubMed2005

Cascading third-order Raman process and local structure formation in binary liquid mixtures of benzene and n-hexane.

The cascading third-order Raman process in binary mixtures of benzene and n-hexane was studied by six-wave mixing coherent anti-Stokes Raman scattering spectroscopy. By examining the concentration dependence of the cascading third-order signal intensity, we investigated the formation of local structures of benzene in the binary mixtures. A significant deviation from the dependence expected for homogeneous mixtures was observed at benzene concentrations above 7 mol dm(-3). This deviation can be interpreted in terms of optical inhomogeneity caused by the formation of domain structures of benzene molecules. We discuss the feasibility of the cascading third-order process as a sensitive probe for the microscopic structures that are formed in liquids and solutions.

Journal Article↗

PubMed2003

Characterization of a supercontinuum generated from a photonic crystal fiber and its application to coherent Raman spectroscopy.

The temporal and spectral profiles of supercontinuum radiation generated from a photonic crystal fiber are evaluated with a polarization-gate frequency-resolved optical gating technique. The supercontinuum is then applied to coherent inverse Raman spectroscopy. A stimulated Raman signal of cyclohexane is observed as an induced absorption signal with an instantaneous response. The Raman signal has a peak at a slight negative delay time, which is explained by perturbed Raman-induced coherence.

Journal Article↗