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Biomedical subjects

Hideo Inaba

Publications and source records attributed to Hideo Inaba.

9 recordsLinked to original sources
PubMed2006

Dose-related effects of direct hemoperfusion using a cytokine adsorbent column for the treatment of experimental endotoxemia.

OBJECTIVE: To clarify the dose-related effects of direct hemoperfusion using a cytokine adsorbent column (CTR) on the mortality and inflammatory responses to endotoxin-induced shock in rats. DESIGN: Prospective and randomized study. SETTING: University research laboratory. SUBJECTS: Male Wistar rats. INTERVENTIONS: Forty-eight rats were injected intravenously with Escherichia coli endotoxin (15 mg/kg over 2 min), and then were randomly allocated to one of the following four groups (n = 12 per group): control group, treated without CTR for 120 min; quarter-dose treatment group, treated with CTR 0.25 ml for 120 min; half-dose treatment group, treated with CTR 0.50 ml; and full-dose treatment group, treated with CTR 1.0 ml. MEASUREMENTS AND RESULTS: Hemodynamics and arterial blood gases were recorded, and mortality and plasma cytokine concentrations were calculated for the 8-h observation period. The mortality rates 8 h after endotoxin injection were 92%, 58%, 42% and 17% for the control column, quarter-dose, half-dose, and full-dose CTR groups, respectively. The increases in plasma cytokine concentrations were smaller in the half-dose and full-dose CTR treatment groups than in the endotoxemic group. CONCLUSIONS: The present study showed that CTR treatment dose-dependently decreased the mortality rate and inhibited inflammatory responses in vivo.

Analysis of Variance↗
PubMed2006

Effects of post-treatment with direct hemoperfusion using a CTR column on mortality and inflammatory responses to endotoxin-induced shock in rats.

BACKGROUND: To clarify the effects of post-treatment with direct hemoperfusion using a CTR column on the mortality and inflammatory responses to endotoxin-induced shock in rats. METHODS: Thirty-six male rats were randomly assigned to 1 of 3 groups (n = 12/group): the endotoxemic group, receiving intravenous Escherichia coli endotoxin (15 mg/kg over 2 min); the control column group, treated without CTR for 120 min at 2 h after endotoxin injection, and CTR-post-treatment group, treated with CTR for 120 min at 2 h after endotoxin injection. Hemodynamics, arterial blood gases, and mortality were recorded for the 8-hour observation period, and plasma cytokine concentrations were measured every 4 h. RESULTS: The mortality rates were 83, 83 and 33% for the endotoxemic, control column, and CTR post-treatment groups, respectively. The increases in IL-6 concentrations were less for the CTR post-treatment group than the other 2 groups. CONCLUSION: The present study shows that CTR post-treatment inhibited hypotension and elevations in IL-6 concentrations, reducing the mortality rate of rats with endotoxin-induced shock.

Animals↗
PubMed2006

A novel adsorbent of circulating bacterial toxins and cytokines: the effect of direct hemoperfusion with CTR column for the treatment of experimental endotoxemia.

OBJECTIVES: The current study examined the ability of a new adsorbent, CTR, to remove enterotoxins, toxic shock syndrome toxin-1 (TSST-1), and cytokines from blood and/or serum in vitro and the effects of the extracorporeal treatment with CTR column on mortality rate and inflammatory responses to endotoxic shock in vivo. DESIGN: Laboratory investigation. SETTING: University and company experimental laboratory. MATERIALS: CTR is composed of porous cellulose beads to which a hydrophobic organic compound with a hexadecyl alkyl chain has been covalently bound to the surface as a ligand. Human/bovine serum and human blood samples in vitro and Male Wistar rats were used. INTERVENTIONS: CTR's ability to adsorb bacterial toxins and cytokines related to sepsis in serum and/or blood was examined with an in vitro batch adsorption protocol. In vivo, male Wistar rats were anesthetized and assigned to one of three groups (n=14 per group): Escherichia coli endotoxin (15 mg/kg intravenously) alone (endotoxemic), apheresis with control column without CTR for 120 mins (control column), or extracorporeal treatment with CTR column for 120 mins (CTR treatment). MEASUREMENTS AND MAIN RESULTS: With use of the CTR adsorbent, the adsorption rates were 50% to 90% for enterotoxins, TSST-1, and cytokines such as TNF-alpha and interleukin (IL)-6 in the batch tests. In vivo, the mortality rates at 8 hrs after endotoxin injection were 92%, 92%, and 14% for the endotoxemic, control column, and CTR treatment groups, respectively. Hypotension and elevated plasma cytokine concentrations and the infiltration of neutrophils of the lungs were less conspicuous in the CTR treatment group than in the other two groups. CONCLUSIONS: CTR, a novel adsorbent, effectively adsorbed small- to middle-sized proteins, such as cytokines, enterotoxins, and TSST-1 in vitro. Direct hemoperfusion apheresis with CTR column reduced mortality and had inhibitory effects on the inflammatory responses during endotoxemia in vivo. These findings suggest that extracorporeal blood purification with CTR column may be available to use for patients with sepsis and/or endotoxemia.

Adsorption↗
PubMed2005

[Role of anesthesiologists is the education of cardiopulmonary resuscitation in local hospitals].

BACKGROUND: In Japanese local hospitals there are not many persons who can give speedy and exact medical treatment in emergency. We investigated a role of anesthesiologists in the education of cardiopulmonary resuscitation (CPR) in local hospitals. METHODS: A total of 3 doctors and 30 nurses completed the questionnaire about the education of CPR after training of advanced cardiac life support (ACLS) in a local hospital. RESULTS: It showed that all doctors and nurses have had some anxiety about the emergency treatment of patients and could understand the importance of CPR. Moreover, most of them answered that they wanted to learn ACLS more, and that the training of ACLS simulation was the most impressive. CONCLUSIONS: It is important for anesthesiologists to teach ACLS and to promote the spread of ACLS in local hospitals.

Anesthesiology↗
PubMed2003

Frequency of ventricular fibrillation as predictor of one-year survival from out-of-hospital cardiac arrests.

This study determined whether electrocardiographic analysis of ventricular fibrillation (VF) can predict 1-year survival from bystander-witnessed, out-of-hospital cardiac arrests of cardiac origin. VF was analyzed using fast-Fourier transformation in a community in which emergency medical technicians delivered shock with an automated external defibrillator before arrival to the hospital. The frequency of VF can predict survival 1 year after hospital discharge from shock-delivered, bystander-witnessed cardiac arrests of cardiac etiology.

Aged↗
PubMed2002

Expression of heme oxygenase-1 in the lung and liver tissues in a rat model of burns.

To investigate the functional significance of heme oxygenase (HO)-1 as having anti-oxidant activity in burns, a rat model of 30% burns was prepared, and the lungs and livers were extirpated for immunohistological examination (indirect immunoenzyme techniques). Plasma TNF-alpha levels were significantly higher in the burn group than in the control group, but there was no significant difference in total bilirubin levels between the groups. HO-1 expression was found histologically in the perivascular cells of the lung and liver. It was also found, though only at low levels, in liver parenchymal cells. These results suggest that HO-1 is expressed in the lung and liver tissues in a rat model of burns.

Animals↗