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Biomedical subjects

Hiromoto Ito

Publications and source records attributed to Hiromoto Ito.

At least 19 recordsLinked to original sources
PubMed2006

De Quervain's disease: efficacy of intra-sheath triamcinolone injection.

The methods and clinical outcomes of intra-sheath triamcinolone injection in the treatment of de Quervain's disease are described. We used 38 hands of 36 patients. A mixture of 1 ml of triamcinolone and 1 ml of 1% lidocaine hydrochloride was injected, with an interval of 2 weeks. The fluid was injected into one point above the induration for the first 18 hands and into two points over the extensor pollicis brevis and abductor pollicis longus tendon in the induration for hands 19-38. The efficacy rate was 89%, with the treatment results of the two-point injection better than those of the one-point injection. Recurrence was observed in ten hands, and complications in 13 hands; however, over 90% of patients were satisfied with the injection. The accurate injection of triamcinolone into the sheath of both the extensor pollicis brevis and abductor pollicis longus tendon was considered very effective for de Quervain's disease.

Adult↗
PubMed2006

Closing radial wedge osteotomy for Preiser's disease: a case report.

A case of Preiser's disease treated with closing radial wedge osteotomy is reviewed and the therapeutic effect is reported. A good result was obtained in the short term and we believe that this surgical method could become one of the choices for surgical treatment to alleviate pain in Preiser's disease.

Arthralgia↗
PubMed2005

The role of bone marrow-derived cells in bone fracture repair in a green fluorescent protein chimeric mouse model.

We investigated the role of bone marrow cells in bone fracture repair using green fluorescent protein (GFP) chimeric model mice. First, the chimeric model mice were created: bone marrow cells from GFP-transgenic C57BL/6 mice were injected into the tail veins of recipient wild-type C57BL/6 mice that had been irradiated with a lethal dose of 10Gy from a cesium source. Next, bone fracture models were created from these mice: closed transverse fractures of the left femur were produced using a specially designed device. One, three, and five weeks later, fracture lesions were extirpated for histological and immunohistochemical analyses. In the specimens collected 3 and 5 weeks after operation, we confirmed calluses showing intramembranous ossification peripheral to the fracture site. The calluses consisted of GFP- and osteocalcin-positive cells at the same site, although the femur consisted of only osteocalcin-positive cells. We suggest that bone marrow cells migrated outside of the bone marrow and differentiated into osteoblasts to make up the calluses.

Animals↗
PubMed2005

The anti-cell death FNK protein protects cells from death induced by freezing and thawing.

The FNK protein, constructed from anti-apoptotic Bcl-xL with enhanced activity, was fused with the protein transduction domain (PTD) of the HIV/Tat protein to mediate the delivery of FNK into cells. The fusion protein PTD-FNK was introduced into chondrocytes in isolated articular cartilage-bone sections, cultured neurons, and isolated bone marrow mononuclear cells to evaluate its ability to prevent cell death induced by freezing and thawing. PTD-FNK protected the cells from freeze-thaw damage in a concentration-dependent manner. Addition of PTD-FNK with conventional cryoprotectants (dimethyl sulfoxide and hydroxyethyl starch) increased surviving cell numbers around 2-fold compared with controls treated only with the cryoprotectants. Notably, PTD-FNK allowed CD34+ cells among bone marrow mononuclear cells to survive more efficiently (12-fold more than the control cells) from two successive freeze-thaw cycles. Thus, PTD-FNK prevented cell death induced by freezing and thawing, suggesting that it provides for the successful cryopreservation of biological materials.

Animals↗
PubMed2005

Association of single nucleotide polymorphisms in the promoter region of the pro-opiomelanocortin gene (POMC) with low bone mineral density in adult women.

Among multiple factors influencing osteoporosis, genetic variations involved in bone-mineral metabolism can affect risks predisposing to the disease onset. Here, we studied single-nucleotide polymorphisms (SNPs) in the pro-opiomelanocortin (POMC) gene for possible association with bone mineral density (BMD) among 384 adult Japanese women and observed significant correlation between adjusted BMD and three SNPs in the promoter region (r>0.14, p<0.01). The most significant correlation was observed for -2353G/A (r=-0.16, p=0.002); homozygous carriers of the major (G) allele had the highest BMD (0.405+/-0.054 g/cm2) while heterozygous carriers were intermediate (0.390+/-0.053 g/cm2) and homozygous A-allele carriers had the lowest BMDs (0.369+/-0.048 g/cm2). Although no association was detected between these SNPs and body weight or body mass index (BMI), significant association was detected between the -2313A/C genotype and plasma total cholesterol level (r=-0.12, p=0.019). We propose that POMC is among the likely susceptibility genes for osteoporosis and may also be involved in dyslipidemia.

Adult↗
PubMed2005

Percutaneous leverage pinning in the treatment of Bennett's fracture.

Twelve patients (eleven males, one female; mean age 36 years) with Bennett's fracture having a gap or a step-off of more than 2 mm and in whom maintenance of the reduced position was difficult were treated by percutaneous leverage pinning. Bone union was obtained in all patients, and the wire was removed 37 days on average after the initial treatment. On the final follow-up examination, mild pain with motion was noted in two patients, and moderate to severe pain was noted in one patient. The reduction of the articular surface of the carpometacarpal joint of the thumb was confirmed by radiography; it was less than 1 mm in nine patients, less than 2 mm in two, and more than 2 mm in one. Because reduction and fixation are performed utilizing the leverage force of the wire inserted into the trapezium, percutaneous pinning has advantages, such as technical simplicity and the ability to apply tension to the fractured site.

Adolescent↗
PubMed2005

Epithelioid hemangioendothelioma of the phalanx: a case report.

Epithelioid hemangioendothelioma (EH) of a hand bone is extremely rare. We describe a patient with a phalangeal EH. This was treated by resection of the entire middle phalanx with no recurrence within a 10-year postsurgical follow-up period. We recommend wide resection as the safest treatment for EH of the hand phalanx.

Bone Neoplasms↗
PubMed2005

Intrasheath triamcinolone injection for the treatment of trigger digits in adult.

The purpose of this study is to describe our technique of intrasheath triamcinolone injection for trigger digit and to evaluate the treatment outcome. Seventy-two patients of 81 digits treated with intrasheath triamcinolone injection were followed up for at least one year and interviewed in our clinic. The injection was performed in all patients by one of the authors at two-week intervals. The amount used was 1 ml (10 mg) of triamcinolone and was mixed with 1 ml of 1% lidocaine and tried precise injection into the tendon sheath. According to the evaluation method developed by Patel and Moradia, they were excellent in 67 digits, good in ten digits, fair in three digits, and poor in one digit. Satisfactory results were obtained in 95% of the digits, and the effectiveness rate was higher than previously reported. But it should also be noted that triamcinolone easily induces local side effects such as dermatitis.

Adult↗
PubMed2005

Electromyographic functional analysis of the lumbar spinal muscles with low back pain.

The lumbar paraspinal muscles were examined by surface electromyography (EMG) in 22 patients with low back pain and 22 healthy volunteers. Surface electrodes were placed bilaterally on the lumbar multifidus and longissimus muscles at the level of the spinous process of the third lumbar vertebra. Kinesiologic EMG was recorded, in standing resting position, during the following trunk motions: trunk forward flexion and extension, lateral bending and axial rotation. No muscular activity was observed in the full trunk flexion position in the control group. In contrast, continuous muscle activity was observed in the low back pain group. On axial rotation, an intermuscular time lag was observed at the beginning of the motion in the control group. In the low back pain group, there was no such time lag. Paraspinal muscle activities restricted lumbar range of motion, and protect from injury for movement. This suggests their role as stabilizers.

Adult↗
PubMed2005

A case report of rapidly progressing cauda equina symptoms due to rheumatoid arthritis.

Although rheumatoid involvement of the lumbar spine is relatively rare, we report a patient with rapidly progressing cauda equina symptoms due to rheumatoid diskitis. A 72-year-old woman was admitted to our hospital because of motor weakness below the iliopsoas muscle and sensory disturbance beneath the level of L2. Plain X-ray films, computed tomography, and magnetic resonance imaging demonstrated destruction of the L2/3 intervertebral disc and endplates with subluxation of the facet joints. The dural sac was compressed. Based on a diagnosis of spinal canal stenosis due to rheumatoid diskitis, we performed partial laminectomy and posterolateral fusion with pedicle screws. The neurological deficits improved immediately. The mechanism of intervertebral disc destruction in this case is thought to be rheumatoid nodes or enthesitis. Destruction of the facet joints and the intervertebral disc might have led to severe instability and spinal canal stenosis.

Aged↗
PubMed2005

Evaluation of the changes in the muscle sympathetic nerve activity and anterior tibial muscle blood flow caused by the Valsalva maneuver in patients with lumbago and healthy subjects.

Clinical symptoms affecting the lower extremities are common among lumber spinal disorder patients. Pain, numbness and sensory disturbance are major signs of these symptoms, and have been suggested to be related to sympathetic nerve disturbance. This study was designed to examine whether these patients experience a difference in sympathetic nerve flow in terms of muscle sympathetic nerve activity (MSA) compared to healthy subjects. Five patients with lumbar intervertebral disc herniation of the spine (LIDH) and four patients with lumbar spinal canal stenosis (LSCS) were examined along with six healthy volunteers. Basic MSAs for IDH and SCS patients that were introduced from a common peroneal nerve were found to be statistically higher than those of the control subjects. MSA behavior and muscle blood flow introduced from the tibialis anterior muscle over 30 seconds while performing the Valsalva maneuver, a well-known technique used to artificially facilitate MSA, were examined for all subjects, and showed relatively slower changes for LIDH and LSCS patients compared to the normal subjects. Muscle blood flow was inversely proportional to MSA for the normal subjects, and this relationship was observed for IDH patients as well as SCS patients. However, MSA and the muscle blood flow of patients gradually changed while performing the Valsalva maneuver relative to the control subjects. This suggests that the systemic physiological response to the maneuver is maintained, but that, some local modification mechanisms exist.

Adult↗
PubMed2005

[Clinical evaluation of low back pain].

On the diagnosis of backache, at first ask patients detailed questions about medical history of backache, the situation at the time of onset and process of the pain, intensity and location on the body of the pain, which movement to strengthen them or which posture to moderate them. Following them, check the abnormality of how to walk and the normal curvature of the spine at standing from behind and the side. Examine the movement and flexibility of the lumbar spine in bending forward, backward and twisting. After them, examine the neurological functions on their legs to search paralysis, muscle weakness, reflex changes and sensory changes. Observe lumbar part and check the presence of redness, swelling and hyperthermia, and examine a location of tenderness. X-rays, CT or MRI examination can be performed if necessary, but should not be fascinated by only image findings, and it is necessary to evaluate in total them with the results of physical examinations.

Diagnostic Imaging↗
PubMed2004

Transduction of anti-apoptotic proteins into chondrocytes in cartilage slice culture.

Peptides of the protein transduction domain (PTD) mediate the introduction of passenger proteins into cells in vitro and in vivo, where the domains are positively charged. This unusual ability can be exploited for medical applications in protein therapeutics. Chondrocytes are embedded in a dense extracellular matrix, whose components are highly negatively charged. We examined whether PTD mediates the delivery of functional proteins into chondrocytes through the matrix using the super anti-apoptotic protein FNK fused with Tat/PTD peptide (PTD-FNK), the FNK protein being constructed from anti-apoptotic Bcl-xL to enhance its activity. The PTD-FNK protein labeled with a fluorescent dye was incorporated into chondrocytes through the matrix and immunostaining confirmed the transduction into the cells. The PTD-FNK protein protected chondrocytes from cell death induced by Fas antibody and nitrogen oxide (NO). Thus, the PTD peptide has the ability to deliver passenger proteins into chondrocytes by penetrating the extracellular matrix of cartilage.

Apoptosis↗
PubMed2004

Oral alendronate treatment for polyostotic fibrous dysplasia: a case report.

Recently, intravenous therapy with pamidronate has been reported to be effective for treating fibrous dysplasia. However, very little is known about the efficacy of oral alendronate for fibrous dysplasia. We describe a patient with polyostotic fibrous dysplasia, who had complained of persistent pain for more than 20 years, that was treated with oral alendronate alone. Follow-up examinations were at intervals of 3 months for 2 years. The pain, radiographic findings, and bone turnover, which was monitored using various markers, improved. No adverse side effect was noted. Oral alendronate is suggested to be a useful option in the treatment of polyostotic fibrous dysplasia.

Administration, Oral↗
PubMed2004

Osteogenic differentiation of human bone marrow-derived mesenchymal cells cultured on alumina ceramics.

Alumina ceramics have excellent mechanical and biocompatible properties, but are bioinert and hence have no bone-bonding properties. We took a tissue-engineering approach in an attempt to modify the ceramic surface and so provide an osteogenic/osteoconductive milieu. We obtained human bone marrow mesenchymal cells from four donors and then cultured the cells for two weeks on alumina ceramic in the presence of beta-glycerophosphate, ascorbic acid and dexamethasone. The cells showed extensive alkaline phosphatase staining and mineralization, as evidenced by Alizarin Red S staining and calcein uptake. Biochemical analyses revealed high levels of alkaline phosphatase activity, osteocalcin expression and calcium content. This data indicates the appearance of active osteoblasts that are concomitant with bone matrix formation, i.e., in vitro cultured bone. The cultured bone/alumina composites should prevent the aseptic loosening of all-alumina ceramic joints or the detachment of implanted alumina ceramics, and thus could have clinical significance in orthopedic reconstructive surgery.

Adult↗
PubMed2004

Experimental study on the effect of mechanical stimulation on the early stage of fracture healing.

In an attempt to ascertain the effects of mechanical stimulation on callus in the early stage of bone fracture healing, a tibial fracture was induced in rats and mechanical stimulation applied to the fractures. The callus was then measured quantitatively, while the fractures were analyzed both radiographically and histologically. Following the induction of a closed transverse fracture in the tibia, external anchors were applied and the rats raised by suspending the fractured leg. The rats were divided into two main groups: a Stimulation Group (S Group) and a Control Group (C Group) without the application of any mechanical stimulation. The S Group was further divided into the following three subgroups: an axial compression group (Sc Group) receiving stimulation in the positive direction; an axial distraction group (Sd Group) receiving stimulation in the negative direction; and an axial dynamization group (Sdy Group) receiving stimulation in both directions alternately. For mechanical stimulation, 1.4-N sine waves were applied continuously for 30 minutes a day, three times a week, starting 2 days after fracture-inducing surgery. At 3, 7, and 14 days after surgery, transverse sections of each fractured bone sample were prepared. At 14 days after surgery, each transverse section was divided into two peripheral and central regions to permit calculation of the area ratio of callus. Radiographically, no marked differences were observed among the groups; histologically, differences were seen 7 days after surgery, suggesting that mechanical stimulation facilitated bone healing soon after surgery. At 14 days after surgery, the amount of callus for the C Group was less than that for all three stimulation groups. In the C Group, the amount of callus in the peripheral region was greater than in the central region, and in the Sc Group, the results were the same: callus in the peripheral region was greater than in the central region. In the Sd Group, callus was greater in the central region than in peripheral regions. In the Sdy Group, favorable callus was observed in both the central and peripheral regions. These findings suggest that axial compression facilitates callus primarily in the peripheral region, while axial distraction facilitates callus primarily in the central region. When axial compression and distraction were alternated (dynamization), callus was significantly facilitated in both the central and peripheral regions. Of the three axial stimulation techniques, dynamization was the most effective in facilitating callus in the early stage of bone fracture healing.

Animals↗
PubMed2004

Anterior cruciate ligament injuries among wakeboarders: a case report.

No previous cases of anterior cruciate ligament (ACL) injuries sustained during wake-boarding have been reported. We report on a case involving an ACL injury sustained during wakeboarding. A 27-year-old man sustained an injury while attempting a wakeboarding maneuver(a heel-side back roll, consisting of a jump and simultaneous roll toward the heel side). He failed to complete his roll before landing, striking the water with his right shoulder foremost, then plunging underwater. When his wakeboard struck the water, his left knee was sprained by the rotational force exerted by the board. The patient was diagnosed with an isolated ACL injury and underwent arthroscopic ACL reconstructive surgery. The board used in wakeboarding is wider and subject to greater water resistance than that used in water skiing. The feet of the wakeboarder are firmly attached by binding boots to a board, laterally with respect to the direction of motion, impeding easy separation of the board from the feet in the event of a fall. Thus, wakeboarding conditions would appear to put wakeboarders at particular risk for ACL injuries. These conditions need be assessed from a medical perspective in order to devise ways to minimize the risk of such injuries.

Adult↗