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Biomedical subjects

Hiroyuki Takeda

Publications and source records attributed to Hiroyuki Takeda.

At least 37 records · Page 2Linked to original sources
PubMed2005

Endothelin-3 controls growth of colonic epithelial cells by mediating epithelial-mesenchymal interaction.

It has been repeatedly reported that endothelin-3 (ET-3) is expressed by gastrointestinal mesenchymes, and that paracrine signaling between ET-3 and its receptor plays an essential role in controlling differentiation of the enteric nervous system in the gut, especially in the colon. However it remains to be solved whether ET-3 plays a role in regulating the growth of gastrointestinal epithelial cells. We have previously reported culture systems for forestomach, glandular stomach and duodenal epithelial cells, but a system for colonic epithelial cells has not been established. In the present study, we examined optimal culture conditions for colonic epithelial cells, and examined whether ET-3 affects the growth of gastrointestinal epithelial cells, with special reference to colonic cells. We found that ET-3 dose-dependently and region-specifically stimulated their growth in primary culture: colonic epithelial cells were most responsive, followed by duodenal and glandular stomach epithelial cells. Reverse transcription-polymerase chain reaction analysis showed that ET-3 and a receptor for ET-3 were expressed by both colonic mesenchymes and epithelia, but the levels were much higher in mesenchymes than in epithelia. These results suggest that ET-3 plays an important role in the growth control of colonic epithelial cells, possibly by mediating epithelial-mesenchymal interactions.

Animals↗
PubMed2004

Zebrafish GADD45beta genes are involved in somite segmentation.

Somites in vertebrates are periodic segmented structures that give rise to the vertebrae and muscles of body. Somites are generated from presomitic mesoderm (PSM), but it is not fully understood how cellular differentiation and segment formation are achieved in the anterior PSM. We report here that zebrafish gadd45beta1 and gadd45beta2 genes are periodically expressed as paired stripes adjacent to the neural tube in the anterior PSM region where presomitic cells mature. In mammals, it is known that GADD45 (growth arrest and DNA damage) family proteins play a role in cell-cycle control. We found that both knockdown and overexpression of gadd45beta genes caused somite defects with different consequences for marker gene expression. Knockdown of gadd45beta genes with antisense morpholino oligonucleotides caused a broad expansion of mesp-a in the PSM, and both cyclic expression of her1 and segmented expression of MyoD were disorganized. On the other hand, injection of gadd45beta1 or gadd45beta2 suppressed expression of mesp-a and her1 in anterior PSM and MyoD in paraxial mesoderm. These results indicate that regulated expression of gadd45beta genes in the anterior PSM is required for somite segmentation.

Amino Acid Sequence↗
PubMed2004

Morphogenesis and regionalization of the medaka embryonic brain.

We examined the morphogenesis and regionalization of the embryonic brain of an acanthopterygian teleost, medaka (Oryzias latipes), by in situ hybridization using 14 gene probes. We compared our results with previous studies in other vertebrates, particularly zebrafish, an ostariophysan teleost. During the early development of the medaka neural rod, three initial brain vesicles arose: the anterior brain vesicle, which later developed into the telencephalon and rostral diencephalon; the intermediate brain vesicle, which later developed into the caudal diencephalon, mesencephalon, and metencephalon; and the posterior brain vesicle, which later developed into the myelencephalon. In the late neural rod, the rostral brain bent ventrally and the axis of the brain had a marked curvature at the diencephalon. In the final stage of the neural rod, ventricles began to develop, transforming the neural rod into the neural tube. In situ hybridization revealed that the brain can be divided into three longitudinal zones (dorsal, intermediate, and ventral) and many transverse subdivisions, on the basis of molecular expression patterns. The telencephalon was subdivided into two transverse domains. Our results support the basic concept of neuromeric models, including the prosomeric model, which suggests the existence of a conserved organization of all vertebrate neural tubes. Our results also show that brain development in medaka differs from that reported in other vertebrates, including zebrafish, in gene-expression patterns in the telencephalon, in brain vesicle formation, and in developmental speed. Developmental and genetic programs for brain development may be somewhat different even among teleosts.

Animals↗
PubMed2004

TE-TM mode coupling in two-dimensional photonic crystals composed of liquid-crystal rods.

We theoretically demonstrate the TE-TM mode coupling in two-dimensional photonic crystals composed of liquid-crystal rods due to anisotropies of liquid crystals. In such structures, the classification of the TE and TM modes is generally impossible, that is, the TE-TM mode coupling occurs. Frequencies of the mode coupling are investigated by the plane-wave expansion method, and the mode coupling is discussed by transmittance calculated by the finite-difference time-domain method. Changes of transmittance by rotating directors of liquid crystals are also discussed.

Journal Article↗
PubMed2004

The zebrafish iguana locus encodes Dzip1, a novel zinc-finger protein required for proper regulation of Hedgehog signaling.

Members of the Hedgehog (Hh) family of intercellular signaling molecules play crucial roles in animal development. Aberrant regulation of Hh signaling in humans causes developmental defects, and leads to various genetic disorders and cancers. We have characterized a novel regulator of Hh signaling through the analysis of the zebrafish midline mutant iguana (igu). Mutations in igu lead to reduced expression of Hh target genes in the ventral neural tube, similar to the phenotype seen in zebrafish mutants known to affect Hh signaling. Contradictory at first sight, igu mutations lead to expanded Hh target gene expression in somites. Genetic and pharmacological analyses revealed that the expression of Hh target genes in igu mutants requires Gli activator function but does not depend on Smoothened function. Our results show that the ability of Gli proteins to activate Hh target gene expression in response to Hh signals is generally reduced in igu mutants both in the neural tube and in somites. Although this reduced Hh signaling activity leads to a loss of Hh target gene expression in the neural tube, the same low levels of Hh signaling appear to be sufficient to activate Hh target genes throughout somites because of different threshold responses to Hh signals. We also show that Hh target gene expression in igu mutants is resistant to increased protein kinase A activity that normally represses Hh signaling. Together, our data indicate that igu mutations impair both the full activation of Gli proteins in response to Hh signals, and the negative regulation of Hh signaling in tissues more distant from the source of Hh. Positional cloning revealed that the igu locus encodes Dzip1, a novel intracellular protein that contains a single zinc-finger protein-protein interaction domain. Overexpression of Igu/Dzip1 proteins suggested that Igu/Dzip1 functions in a permissive way in the Hh signaling pathway. Taken together, our studies show that Igu/Dzip1 functions as a permissive factor that is required for the proper regulation of Hh target genes in response to Hh signals.

Amino Acid Sequence↗
PubMed2004

Tunable photonic band gaps in two-dimensional photonic crystals by temporal modulation based on the Pockels effect.

We theoretically demonstrate the tuning of photonic band gaps in two-dimensional photonic crystals composed of rods by temporal modulation based on the Pockels effect. Refractive indices of rods can be changed periodically by applied alternating voltage under the influence of the Pockels effect, which causes new photonic band states other than original ones. Therefore, photonic band gaps can be closed by the new photonic band states, which may provide novel applications for tunable photonic crystals.

Journal Article↗
PubMed2004

Early fin primordia of zebrafish larvae regenerate by a similar growth control mechanism with adult regeneration.

Some vertebrate species, including urodele amphibians and teleost fish, have the remarkable ability of regenerating lost body parts. Regeneration studies have been focused on adult tissues, because it is unclear whether or not the repairs of injured tissues during early developmental stages have the same molecular base as that of adult regeneration. Here, we present evidence that a similar cellular and molecular mechanism to adult regeneration operates in the repair process of early zebrafish fin primordia, which are composed of epithelial and mesenchymal cells. We show that larval fin repair occurs through the formation of wound epithelium and blastema-like proliferating cells. Cell proliferation is first induced in the distal-most region and propagates to more proximal regions, as in adult regeneration. We also show that fibroblast growth factor signaling helps induce cell division. Our results suggest that the regeneration machinery directing cell proliferation in response to injury may exist from the early developmental stages.

Age Factors↗
PubMed2004

Possible roles of zic1 and zic4, identified within the medaka Double anal fin (Da) locus, in dorsoventral patterning of the trunk-tail region (related to phenotypes of the Da mutant).

Double anal fin (Da) is a spontaneous medaka mutant that exhibits an unique ventralizing phenotype, a mirror-image duplication across the lateral midline in the dorsal trunk-tail region. In the mutant, early D-V specification appears normal but the altered phenotype becomes evident during late embryogenesis. In this study, we genetically specified the mutation to a 174-kb region harboring two zinc-finger type transcription factors, zic1 and zic4, and compared the genomic structures of this region between wild-type and Da mutant fish. No mutation was found in the coding regions of either gene of the mutant, while two fragments, 324 bp and 3-4 kb long, were found inserted downstream of zic1 and zic4, respectively. Probably as a result of this, the expression of both genes is lost in the derivatives of the dorsal (epaxial) somite and the region dorsal to the terminal axis bending. All these tissues are morphologically affected or become ventralized in the mutants. In contrast, the expression in the head region and dorsal spinal cord remained unchanged. Detailed characterization of Da phenotypes revealed a novel defect in the axial skeleton (spina bifida occulta) that was also found in zic1-deficient mice. Finally, zic1-morpholino injection partially phenocopied early Da phenotypes. These findings strongly suggest that zic1 and/or zic4 are required for dorsal identity in the trunk-tail region and that loss of their expression in the epaxial somite derivatives and tail region causes the Da phenotypes.

Animals↗
PubMed2004

Characterization of mutations affecting embryonic hematopoiesis in the medaka, Oryzias latipes.

In a genetic screen for mutations affecting organogenesis in the medaka, Oryzias latipes, we identified eight mutants with defects in embryonic hematopoiesis. These mutations were classified into seven complementation groups. In this paper, we characterize the five mutants that were confirmed in the next generation. The beni fuji mutant was defective in the generation of blood cells, exhibiting reduced blood cells at the initiation of circulation. Mutations in two genes, lady finger and ryogyoku, caused abnormal morphology of blood cells, i.e., deformation, along with a progressive decrease in the number of blood cells. The sekirei mutant exhibited photosensitivity with autofluorescent blood cells. Mutations in kyoho resulted in huge blood cells that were approximately three times longer than the wild-type blood cells. The spectrum of phenotypes identified in this study is similar to that of the zebrafish hematopoietic mutants except for the huge blood cells in kyoho. Our results demonstrate that medaka, as well as zebrafish, is a useful model to study hematopoiesis.

Animals↗
PubMed2004

Medaka genomics: a bridge between mutant phenotype and gene function.

Recent advances in medaka genetics have proven that the medakafish is an excellent model system for developmental and evolutionary biology studies and that it can complement similar studies in zebrafish. Large-scale mutagenesis projects are now being conducted by several groups in Japan and are delivering a vastly expanded pool of medaka mutant stocks. This growing availability of genomic resources will greatly accelerate progress in moving from mutant phenotypes to the elucidation of gene function. This phenotype-driven approach can be expected to lead to the identification and characterization of novel genes and pathways in vertebrate genomes. This review discusses the current state of medaka genomic resources, the state of medaka gene mapping and medaka genome sequencing projects.

Animals↗
PubMed2004

Molecular cloning of two exo-beta-glucanases and their in vivo substrates in the cell walls of lily pollen tubes.

Full-length cDNA sequences of two exo-beta-glucanases, LP-ExoI and LP-ExoII, secreted into cell walls of lily (Lilium longiflorum) pollen tube, were determined by RT-PCR. LP-ExoI exhibited over 80% similarity to LP-ExoII at both DNA and amino acid levels. RT-PCR showed that LP-ExoI transcripts were abundant in pollen grains and tubes, but could not be detected in leaf, stem, stigma, style, ovary, petal, filament, young root, young bud, and scale leaf of bulb. However, LP-ExoII transcripts ubiquitously existed in all the tissues tested. To determine the potential substrates of exo-beta-glucanases, cell wall components of lily tissues were analyzed. Linkage analysis revealed that pollen tubes contained high levels of 3-Glc in hemicellulose (44.3%), while pollen grains had no detectable 3-Glc. The hemicellulose fraction of pollen tubes was treated with lichenase and the product was analyzed by HPLC-PAD to determine the origin of 3-Glc. Specific tetra-saccharide was liberated from hemicellulose of pollen tubes, suggesting the presence of 1,3 : 1,4-beta-glucan in lily pollen tube hemicellulose. The structure of this 1,3 : 1,4-beta-glucan may be different from cereal plant 1,3 : 1,4-beta-glucan, since tri-saccharide was not detected in hemicellulose fraction after lichenase treatment. LP-ExoI and LP-ExoII, expressed in pollen grains and tubes, may be involved in the regulation of pollen tube elongation by hydrolyzing callose and 1,3 : 1,4-beta-glucan within pollen tube walls.

Amino Acid Sequence↗
PubMed2004

Telomere length in leukocytes and cultured gingival fibroblasts from patients with aggressive periodontitis.

BACKGROUND: The association of genetic risk factors with the pathogenesis of aggressive periodontitis (AgP) has been a focus of attention. Telomeres, which are nucleoprotein complexes at the ends of chromosomes, could be a genetic marker for Down's syndrome and Hutchinson-Gilford progeria, in which patients' premature aging is involved in the pathogenesis. It has been reported that these patients tend to experience severe periodontitis. Therefore, we investigated the telomere length of peripheral blood leukocytes (PBL) obtained from patients with AgP and that in the patients' gingival fibroblasts undergoing cellular aging in vitro. METHODS: Twenty-one patients with AgP and 50 age-matched, periodontally healthy subjects (HS) participated in this study. Genomic DNA from PBL and from human gingival fibroblasts (HGF) was analyzed by Southern blotting for telomere length. The percentage of HGF positive for beta-galactosidase (beta-gal), a marker for cellular senescence, was also investigated. RESULTS: There was no significant difference in the telomere length (P = 0.20, Student's t test) between the two groups, and wide interindividual variation was found (5.93 to 11.4 kbp, average 8.35 +/- 1.19 kbp). The telomere length from PBL negatively correlated with donor age, but no significant difference in telomere loss between the two groups was observed. With HGF undergoing aging in culture, the mean telomere length of these cells from six patients with AgP and seven HS decreased an average of -67.5 bp and -81.0 bp, respectively. No association was found in the telomere length between PBL and HGF from the same donors (r = 0.56, P = 0.20). A significant association was found between the telomere length and the percentages of beta-gal-positive HGF during cell passages (r = 0.70, P < 0.001). CONCLUSIONS: These results indicate that patients with AgP do not have excessive telomere loss and thus do not support the notion of the occurrence of a generalized premature cellular aging in patients with AgP. Further studies are required to investigate the association between telomere length and beta-gal in HGF.

Acute Disease↗
PubMed2003

Coupling of the TE and TM modes of electromagnetic waves in two-dimensional photonic crystals with surface defects of liquid crystals.

We theoretically demonstrate the coupling of the TE and TM modes of electromagnetic waves in two-dimensional photonic crystals with surface defects of liquid crystals. Due to anisotropies of liquid crystals, the TE and TM modes cannot be classified generally in the surface defects, which causes the coupling of the TE and TM modes. The coupling of the TE and TM modes occurs strongly at frequencies at which group velocities of electromagnetic waves become zero, especially at surface defect modes. Possibility of the sharp tunability and the switching of the transmittance in this system has been demonstrated theoretically by the control of directors of liquid crystals by applied electric field due to their anisotropy.

Journal Article↗
PubMed2003

Disappearances of uncoupled modes in two-dimensional photonic crystals due to anisotropies of liquid crystals.

We demonstrate disappearances of uncoupled modes in two-dimensional photonic crystals due to anisotropies of liquid crystals theoretically. Mirror symmetry disappears in wave vectors by rotating directors of liquid crystals, which results in disappearances of uncoupled modes that cannot be excited by external plane waves. This property may provide large tunabilities in two-dimensional photonic crystals utilizing liquid crystals.

Journal Article↗
PubMed2003

Tunable refraction effects in two-dimensional photonic crystals utilizing liquid crystals.

Tunable refraction effects in two-dimensional photonic crystals utilizing liquid crystals are theoretically demonstrated. Due to liquid crystals with anisotropies, the incident light propagates in photonic crystals at refractive angles different from those in photonic crystals composed of isotropic materials. Moreover, refractive angles can be changed by rotating the directors of liquid crystals. Tunable refraction effects are also discussed for the light with two kinds of frequencies: one is a frequency at the edge of a band gap, and the other is a frequency a little far from the edge of a band gap.

Journal Article↗
PubMed2003

The prickle-related gene in vertebrates is essential for gastrulation cell movements.

Involving dynamic and coordinated cell movements that cause drastic changes in embryo shape, gastrulation is one of the most important processes of early development. Gastrulation proceeds by various types of cell movements, including convergence and extension, during which polarized axial mesodermal cells intercalate in radial and mediolateral directions and thus elongate the dorsal marginal zone along the anterior-posterior axis [1,2]. Recently, it was reported that a noncanonical Wnt signaling pathway, which is known to regulate planar cell polarity (PCP) in Drosophila [3,4], participates in the regulation of convergent extension movements in Xenopus as well as in the zebrafish embryo [5-8]. The Wnt5a/Wnt11 signal is mediated by members of the seven-pass transmembrane receptor Frizzled (Fz) and the signal transducer Dishevelled (Dsh) through the Dsh domains that are required for the PCP signal [6-8]. It has also been shown that the relocalization of Dsh to the cell membrane is required for convergent extension movements in Xenopus gastrulae. Although it appears that signaling via these components leads to the activation of JNK [9,10] and rearrangement of microtubules, the precise interplay among these intercellular components is largely unknown. In this study, we show that Xenopus prickle (Xpk), a Xenopus homolog of a Drosophila PCP gene [11-13], is an essential component for gastrulation cell movement. Both gain-of-function and loss-of-function of Xpk severely perturbed gastrulation and caused spina bifida embryos without affecting mesodermal differentiation. We also demonstrate that XPK binds to Xenopus Dsh as well as to JNK. This suggests that XPK plays a pivotal role in connecting Dsh function to JNK activation.

Adaptor Proteins, Signal Transducing↗
PubMed2003

Expression and function of cell wall-bound cationic peroxidase in asparagus somatic embryogenesis.

Cultured asparagus (Asparagus officinalis L. cv Y6) cells induced to regenerate into whole plants through somatic embryogenesis secreted a 38-kD protein into cell walls. The full-length cDNA sequence of this protein (Asparagus officinalis peroxidase 1 [AoPOX1]) determined by reverse transcriptase-polymerase chain reaction showed similarity with plant peroxidases. AoPOX1 transcripts were particularly abundant during early somatic embryogenesis. To evaluate the in vivo function of AoPOX1 protein, purified recombinant AoPOX1 protein was reacted with a series of phenolic substrates. The AoPOX1 protein was effective in the metabolism of feruloyl (o-methoxyphenol)-substituted substrates, including coniferyl alcohol. The reaction product of coniferyl alcohol was fractionated and subjected to gas chromatography-mass spectrometry analysis and (1)H-nuclear magnetic resonance analysis, indicating that the oxidation product of coniferyl alcohol in the presence of AoPOX1 was dehydrodiconiferyl alcohol. The concentration of dehydrodiconiferyl alcohol in the cultured medium of the somatic embryos was in the range of 10(-8) M. Functions of the AoPOX1 protein in the cell differentiation are discussed.

Amino Acid Sequence↗