PubMed Health⌕ Search

Biomedical subjects

Hiroyuki Takeuchi

Publications and source records attributed to Hiroyuki Takeuchi.

At least 37 records · Page 2Linked to original sources
PubMed2005

Blood loss estimation during laparoscopic myomectomy using HemoCue.

During laparoscopic myomectomy, it is difficult to determine the actual blood loss volume because physiological saline is used for lavaging to detect the bleeding point or to ensure no active bleeding site. We attempted to estimate blood loss by measuring the waste irrigation fluid's hemoglobin (Hb) concentration using HemoCue (HemoCue, Angelholm, Sweden). Twenty-three women enrolled in our study. After the end of laparoscopic myomectomy, the waste irrigation fluid's Hb content was measured using HemoCue. We estimated blood loss using the following formula. Estimated blood loss (ml)= [waste irrigation fluid volume (ml)]x[Hb concentration in waste irrigation fluid (g/l)]/[patient's preoperative Hb level (g/l)]. The demographic data of the patients showed 35.4 years in age, 159.6 cm in height, and 50.7 kg in weight (mean). The total lavage fluid's Hb level ranged between 0.2 approximately 4.7 (g/l). The total blood loss estimation was calculated and ranged between 17.6 approximately 725.2 ml. There was no significant correlation between the calculated blood loss and the difference in Hb level (Hb level on the first postoperative day minus preoperative Hb level). The HemoCue needs only 10 mcl of blood and it takes only about 45 seconds to produce the result. Furthermore, the HemoCue is a reliable device for measurement of blood hemoglobin concentration. Our method is easy and quick to perform, and blood loss estimation and surgeon's experience (subjective method) show accordance.

Blood Loss, Surgical↗
PubMed2005

Parallel imaging of head with a dedicated multi-coil on a 0.4T open MRI.

BACKGROUND: Parallel imaging is widely used for cylindrical magnetic resonance imaging (MRI); however, few studies apply parallel imaging to open MRI. We previously developed a parallel method called "RAPID" (rapid acquisition through a parallel imaging design) for imaging the heart on a 0.7T open MRI apparatus, and we have now developed a RAPID head coil and shading correction algorithm for imaging the brain with a 0.4T open MRI apparatus. Images acquired with RAPID were compared with those acquired using a conventional quadrature-detection (QD) head coil. MATERIALS AND METHODS: The images were acquired using a dedicated 4-channel RF receiving coil consisting of a solenoid coil and surface coils. For MRI of the brain, we developed 2 methods to acquire the necessary calibration data: a pre-scan method that acquires the calibration data before the main scans and a self-calibration method that acquires the calibration data and imaging data simultaneously. We also modified the algorithm for calculating the shading distribution so that it only uses acquired image data and then corrects the shading. RESULTS: RAPID was applied for T1-weighted, T2-weighted, fluid-attenuation inversion recovery (FLAIR), time-of-flight (TOF), and diffusion-weighted echo-planar (DW-EPI) imaging. The RAPID images had no visible unfolded artifacts or motion artifacts. Images with the same contrast as that with a conventional QD coil were acquired using the RAPID coil and shading correction. CONCLUSION: These preliminary results show that RAPID can be applied to imaging of the head using a 0.4T open MRI apparatus.

Algorithms↗
PubMed2005

[Roles of BPH impact index in the evaluation of impaired urination in patients with BPH].

OBJECTIVE: To assess the significance and current status of the benign prostatic hyperplasia (BPH) impact index (BII) in the evaluation of subjective symptoms of impaired urination in so-called QOL disease, BPH. PATIENTS AND METHODS: Over the past 2 year-period, in 159 patients with the diagnosis of BPH were asked to reply to each of the international prostate symptom score (I-PSS), QOL index and BII questionnaires. The subjective symptom scores (a total of 246 points) were evaluated from the viewpoint of clinical statistics in the search for any these and to find which questions cover the BII, most. RESULTS: 1) Statistically significant but moderate correlations were observed among I-PSS total score, QOL index and BII. The correlations among Qmax, BII and QOL were very weak. 2) Out of the 11 domains in both IPSS and BII, 2 questions of BII ("bothersomeness caused by urinary problems" and "degree of worry about well-being") and 4 questions of IPSS ("residual sense," " pollakisuria," "weak urinary stream" and "nocturia") were shared as QOL indices. Patient satisfaction was affected also by the questions in the BII. 3) Of the 7 BPH symptoms assessed in IPSS, 4 symptoms ("residual sense," "pollakisuria," "weak urinary stream" and "nocturia") affected the QOL index, and 4 symptoms ("urgency on micturition," "residual sense," "nocturia" and "strain at urination") affected BII. 4) Of the 7 symptoms assessed by IPSS, different symptoms affected each of the 4 BII questions. CONCLUSION: It is needed to assess BPH symptoms not only by the IPSS and QOL index but also based on BII to provide the detailed therapeutic instructions and thorough patients consultation.

Aged↗
PubMed2005

[Laparoscopic surgery for gynecologic malignancy].

The gynecologic laparoscopic surgery that has developed in the reproductive field is performed for most cases of benign disease. Introduction of laparoscopic surgery for a gynecologic malignancy is relatively late in comparison with other surgical regions and does not qualify for insurance. An operation for a gynecologic malignancy is classified roughly into a surgery of internal pelvic organs and lymph nodes. The techniques which extensive technology is pursued involve radical hysterectomy and paraaortic lymph node excision. In the surgeons who performed many laparoscopic surgeries, it seems that a surgical procedure other than these can be performed at present. The experienced laparoscopic techniques and the particular knowledge of pelvic anatomy were necessary to perform laparoscopic surgery for gynecologic malignancy.

Female↗
PubMed2005

Adhesion-prevention effects of fibrin sealants after laparoscopic myomectomy as determined by second-look laparoscopy: a prospective, randomized, controlled study.

OBJECTIVE: To examine the adhesion prevention effects of 2 types of fibrin sealant after laparoscopic myomectomy (LM). STUDY DESIGN: A prospective, randomized study (Canadian Task Force I) was conducted at a University-affiliated hospital. A total of 91 patients showing a minimal myoma > 5 cm, excluding pedunculated myomas, underwent LM alone: 32 patients in the control group, 29 in the fibrin gel group and 30 patients in the fibrin sheet group. After LM, postoperative adhesions were evaluated by second-look laparoscopy. The frequency of postoperative adhesions was the main outcome. RESULTS: The frequency of postoperative adhesions of the uterus was significantly lower (p < 0.05) in the fibrin gel group, with 20/32 (62.5%) in the control group, 10/29 (34.5%) in the fibrin gel group and 20/30 (67.7%) in the fibrin sheet group. Although no significant differences were found in the incidence of de novo adnexal adhesions, the lowest rate was found in the fibrin gel group, with 4/32 patients (12.5%) in the control group, 2/29 patients (6.8%) in the fibrin gel group and 5/30 patients (16.7%) in the fibrin sheet group. No bilateral adnexal adhesions were observed in the 3 groups. CONCLUSION: After LM for myomas as large as > or = 5 cm, postoperative adhesions were observed in > or = 50% of patients. The use of fibrin gel after LM is recommended.

Adult↗
PubMed2004

Cardiac cine parallel imaging on a 0.7T open system.

BACKGROUND: Parallel imaging can be applied to cardiac imaging with a cylindrical MRI (magnetic resonance imaging) apparatus. Studies of open MRI, however, are few. This study sought to achieve cardiac cine parallel imaging (or RAPID, for "rapid acquisition through parallel imaging design") with an open 0.7T MRI apparatus. MATERIALS AND METHODS: Imaging time was shortened in all slice directions with the use of a dedicated four-channel RF receiving coil comprising solenoid coils and butterfly coils. Coil shape was designed through an RF-coil simulation that considered biological load. The auto-calibration of a 0.7T open MRI apparatus incorporated a modified image-domain reconstruction algorithm. Cine images were obtained with a BASG, or balanced SARGE (steady-state acquisition with rewound gradient echo), sequence. Image quality was evaluated with cylindrical phantoms and five healthy volunteers. RESULTS: Multi-slice phantom images showed no visible artifacts. Cine images taken under breath-hold with an acceleration factor of two were evaluated carefully. With auto-calibration, the images revealed no visible unfolded artifacts or motion artifacts. RAPID thus improved the acquisition speed, time resolution, and spatial resolution of short-axis, long-axis, and four-chamber images. CONCLUSION: The use of a dedicated RF coil enabled cardiac cine RAPID to be performed with an open MRI apparatus.

Artifacts↗
PubMed2004

Effects of structured medium- and long-chain triacylglycerols in diets with various levels of fat on body fat accumulation in rats.

The effects of structured medium- and long-chain triacylglycerols (MLCT) in diets containing 50-200 g fat/kg on body fat accumulation were compared with those of long-chain triacylglycerols (LCT) in rats. In rats fed ad libitum, weights of intra-abdominal adipose tissues and carcass fat contents were significantly smaller (P<0.05) in rats fed the 150-200 g MLCT/kg diet than in rats fed 150-200 g LCT/kg diet. Serum and liver triacylglycerol contents were significantly greater (P<0.05) in rats fed 200 g MLCT/kg diet, as were hepatic capacities of citrate synthase and cytochrome oxidase (P<0.05). The effects of MLCT on body fat were also examined in adult rats fed a limited amount of food (approximately 50 % of ad libitum intake). Reduction of body fat deposition during the food restriction was the same between in LCT and MLCT groups. These results suggest that accumulation of body fat was less efficient during long-term feeding of MLCT than LCT in rats fed high-fat diets ad libitum. The effect of MLCT on body fat might be influenced by the dietary fat content or by energy sufficiency.

Adipose Tissue↗
PubMed2004

Association of bone mineral density with vitamin D and estrogen receptor gene polymorphisms during GnRH agonist treatment.

AIMS: This study examined whether or not a decrease in bone mineral density (BMD) induced by the use of gonadotropin-releasing hormone agonist (GnRHa) during sexual maturation is affected by vitamin D receptor and/or estrogen receptor gene polymorphisms, like the phenomenon observed during the postmenopausal period. METHODS: In 43 patients who received GnRHa therapy for 6 months to treat uterine myoma or endometriosis at our department and who were confirmed to have pituitary down-regulation, we measured bone density before and after GnRHa treatment using DXA and analyzed the bone metabolism turnover using bone metabolic markers. Polymorphisms were analyzed by RFLP using FokI and TaqI for the vitamin D receptor gene and PvuII and XbaI for the estrogen receptor gene. The then determined gene polymorphism was analyzed in relation to the percentage decreases in BMD following GnRHa treatment. RESULTS: The patients were divided by f, t into two groups: (f, t) < 2 (Group V-I) and (f, t) > or = 2 (Group V-II). They were also divided by P, x into two groups (P, x) < 3 (Group E-I) and (P, x) > or = 3 (Group E-II). The BMD change was significantly higher in Group V-II than in Group V-I. Group E-II tended to have a higher BMD change than Group E-I, although this difference was not statistically significant. CONCLUSION: Patients who often have f and t polymorphism are more likely to show BMD reduction following GnRHa therapy, like the phenomenon seen during the postmenopausal period, than patients with other gene polymorphisms. Measures to avoid BMD reduction are required when using GnRHa in such patients.

Adult↗
PubMed2004

New monoterpene glucoside from the aerial parts of thyme (Thymus vulgaris L.).

A new monoterpene glucoside (1) was isolated from a methanol extract of the dried aerial parts of thyme (Thymus vulgaris L.), together with known 2- and 5-beta-D-glucopyranosylthymoquinols (2 and 3, respectively), and (-)-angelicoidenol-beta-D-glucopyranoside (4). The structure of 1 was elucidated to be (R)-p-cymen-9-yl beta-D-glucopyranoside by spectral evidence and enantioselective synthesis from (R)- and (S)-p-cymen-9-ol derived from p-cymen-8-ol.

Glucosides↗
PubMed2004

[XY type gonadal dysgenesis, trisomy X and variants].

Sex determination and differentiation depend on differentiation of the indifferent gonad to the testis or ovary, which leads to masculine or feminine differentiation of internal and external genitalia. Recently, genes involved in this cascade have been identified with the advance of molecular genetical analysis. XY gonadal dysgenesis, this is a condition that has XY chromosome but is characterized by the indifferent testis. There are complete and incomplete types. Complete type has bilateral gonads of cordee, does not show physical characteristics of Turner's syndrome, has the uterus and ovaries, and has the vagina in female type, though the external genitalia are immature. Incomplete type is characterized by bilateral testicular hypoplasia(male pseudohermaphroditism) or unilateral testicular hypoplasia and bilateral restiform gonads(mixed gonadal dyspenesis), and the sexuality of the external genitalia is unclear. XX gonadal dysgenesis, complete type is characterized by bilateral restiform gonads, female type internal and external genitalia and sexual immaturity, though it does not show any characteristics of Turner's syndrome. It presents hypergonadotropic hypogonadism endocrinologically. It shows a familial incidence with autosomal recessive inheritance, and sensorineural deafness is accompanied in some cases. Incomplete type has rudimentary ovaries and show a varying degree of secondary sexual characteristics. Mixed dysgenesis, many cases have XO/XY mosaic and this dysgenesis is characterized by unilateral hypoplastic testis and contralateral restiform gonad. It may occur in cases of incomplete type XY gonadal dysgenesis. Trisomy X, cases of trisomy X have three X chromosomes as this term indicates. There are some cases of polisomy with four or more X chromosomes. The frequency of trisomy X has been reported to be one in 1,000 births of female, which means that it is a relatively common chromosomal aberration. It has been reported that about 20% of cases of trisomy X have sexual dysfunction, predominantly with primary amenorrha.

Chromosomes, Human, X↗
PubMed2003

Fibrosis and smooth muscle metaplasia in rectovaginal endometriosis.

Rectovaginal (RV) endometriosis presents with a nodular lesion composed of fibromuscular and endometriotic tissue, and the fibromuscular tissue is the major component in the severe stage. The purpose of our study was to examine the extending process of fibromuscular tissue in RV endometriosis. Histological examinations using immunostains, were performed in 90 RV tissue specimens from 37 women. Fibrosis was present in 89 specimens. In each specimen, the intensity of the fibrosis differed from area to area: in mildly fibrotic areas, the collagen fibers were present around the endometriotic tissue, and in severely fibrotic areas, the fibrosis widely extended into fat and connective tissus as well as within the endometriotic tissue. In the 60 specimens containing endometriotic tissue, the increase in the amount of endometriotic tissue significantly correlated to the increase in degree of fibrosis in the entire tissue. The presence of aggregated smooth muscles, unassociated with blood vessels, was defined as smooth muscle metaplasia (SMM), which was always present within the fibrotic areas, and was observed in 80 specimens. The degree of SMM in the entire tissue was significantly correlated with the degree of fibrosis. From these findings, the following was hypothesized. Initially, endometriotic tissue was present sporadically and fibrosis was present around the endometriotic tissue. Thereafter, proliferation of endometriotic tissue and an increase in fibrosis occur consecutively. The SMM was present within the fibrotic areas, and it became more severe, correlating with the increase in fibrosis. In conclusion, this is the first report describing the extending process of the fibromuscular tissue of RV endometriosis from a histological viewpoint, and we think that recognization of this process is useful for histological diagnosis and clinical management of RV endometriosis.

Actins↗
PubMed2002

Evaluation of adhesion formation after laparoscopic myomectomy by systematic second-look microlaparoscopy.

STUDY OBJECTIVE: To evaluate adhesion formation after laparoscopic myomectomy by second-look laparoscopy using a microlaparoscope. DESIGN: Prospective, nonrandomized study. (Canadian Task Force classification II-1). SETTING: University hospital. PATIENTS: One hundred fifteen women who underwent laparoscopic myomectomy and 51 who underwent second-look minilaparoscopy. INTERVENTION: Laparoscopic myomectomy, in which fibrin glue spray was applied to prevent postoperative adhesion formation, and second-look laparoscopy. MEASUREMENTS AND MAIN RESULTS: The mean interval between surgeries was 5.1 +/- 3.0 months (range 2-18 mo). The mean size of enucleated myomas was 6.1 +/- 1.5 cm (range 3.0-10.5 cm), and mean number of myomas removed/patient was 3.0 +/- 2.2 (range 1-9 myomas). At assessment of 152 myomectomy sites, the rate of adhesions was 29.4%/patient and 11.2%/myomectomy site. Risk factors that influenced adhesion formation were posterior location and intramural myoma. In most cases the organ adhered to the myomectomy site was sigmoid colon. The frequency of adnexal adhesions was 17.6%/patient and 9.8%/site. CONCLUSION: The rate of adhesion formation after laparoscopic myomectomy was low, and routine second-look microlaparoscopy was useful to evaluate the efficacy of the first surgery.

Adult↗
PubMed2002

Body fat accumulation is greater in rats fed a beef tallow diet than in rats fed a safflower or soybean oil diet.

The effects of dietary fats , consisting of different fatty acids, on body fat accumulation and uncoupling protein (UCP) in interscapular brown adipose tissue were studied in rats. Metabolisable energy in experimental diets based on safflower oil, soybean oil or beef tallow was measured strictly (experiment 1). Male Wistar rats were then meal-fed an isoenergetic diet for 8 weeks (experiment 2). Each group of rats showed the same weight gain during the 8-week experimental period. Carcass fat content was greater in rats fed the beef tallow diet than in those fed the with the safflower or soybean oil diets, whereas the weight of abdominal adipose tissue was the same for all three dietary groups. Gene expression of UCP1 and the UCP content of the interscapular brown adipose tissue was lower in the beef tallow diet group than in the other dietary groups. A negative correlation was observed between carcass fat content and n-6 unsaturated fatty acid content in dietary fats. These results suggest that the greater body fat accumulation in rats fed the beef tallow diet results from lower expression of UCP1 mRNA and lower UCP content in brown adipose tissue. n-6 Polyunsaturated fatty acids may be the most effective fatty acids in limiting body fat.

Adipose Tissue↗
PubMed2002

Effects of dietary medium-chain triacylglycerols on serum lipoproteins and biochemical parameters in healthy men.

The objective of this study was to investigate effects of dietary medium-chain triacylglycerols (MCTs) on serum lipid levels, liver function, and hepatic fat accumulations in healthy men. Eleven subjects consumed 2200-2600 kcal daily, of which 70-80 g was fat; the fat included 40 g of MCTs or else 40 g of long-chain triacylglycerols (blended vegetable oil). The diet was followed for 4 weeks in this controlled double-blind study. At the end of the experiment, significant differences were not found in the concentrations of serum total cholesterol, very low density lipoprotein cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol between the groups. Serum triglycerol levels were not significantly different in the groups. Adverse effects from ingestion of MCTs on liver functions, the liver-to-spleen ratio on computed tomography (an index of fatty liver), or results of blood tests were not seen. The results suggest that the long-term effects of dietary MCTs on serum cholesterol were similar to those of unsaturated fatty acids found abundantly in vegetable oil, and that consumption of MCTs in the amount of 40 g/day for a month does not cause liver fat accumulation or liver dysfunction.

Adult↗
PubMed2002

Effect of triacylglycerols containing medium- and long-chain fatty acids on serum triacylglycerol levels and body fat in college athletes.

Triacylglycerols containing medium- and long-chain fatty acids (TML) have medium- and long-chain fatty acids in the same molecule. The effects of dietary TML on serum lipid levels and body fat were studied in six young men belonging to a university rowing club. A double-blind crossover study was performed in which for 3 wk the subjects ingested a liquid diet containing 20 g/d of soybean oil or TML in addition to their regular diets. Throughout the study, they were asked to keep their usual lifestyle, including diet and physical activity. The body composition of the subjects was measured weekly. Blood samples were taken at 0, 2, and 3 wk of each treatment period. There was no significant difference in energy intake between the soybean oil diet period and the TML diet period. The rate of variation of serum triacylglycerol concentration was significantly lower after a consumption of the TML liquid diet for 3 wk compared with the soybean oil liquid diet. The rate of variation of body fat mass was also significantly lower after a consumption of the TML liquid diet for 3 wk compared with the soybean oil liquid diet. However, the serum cholesterol concentration did not change significantly during either dietary treatment. These results suggest that TML, compared with soybean oil, may have the potential to prevent hypertriglyceridemia and obesity caused by consumption of a high-fat diet.

Adipose Tissue↗
PubMed2002

Larger diet-induced thermogenesis and less body fat accumulation in rats fed medium-chain triacylglycerols than in those fed long-chain triacylglycerols.

It has been previously shown that a diet containing medium-chain triacylglycerols (MCT) leads to less body fat accumulation as compared to a diet containing long-chain triacylglycerols (LCT). We investigated the involvement of diet-induced thermogenesis in the accumulation of body fat in rats fed a diet containing MCT. Twelve male Wistar rats were administered 1 g of MCT or LCT by gavage, and their oxygen consumption was measured for 6 h (experiment 1). Forty male Wistar rats were fed a diet containing 10% MCT or LCT for 6 wk, and their body composition was determined (experiment 2). In experiment 1, oxygen consumption increased to a greater extent after MCT administration than after LCT administration. Diet-induced thermogenesis was significantly (0.67 +/- 0.14 kcal) larger after the administration of 1 g of MCT. In experiment 2, there were no differences in food intake or carcass protein content between the LCT group and MCT group. However, carcass fat and intra-abdominal fat content were significantly lower in rats fed MCT than in those fed LCT. We calculated that ingestion of 1 g of MCT decreased body fat by 0.94 +/- 0.27 kcal relative to the ingestion of LCT. These results suggest that the larger diet-induced thermogenesis observed in rats fed MCT, compared to that of those fed LCT, is one of the main factors involved in the suppression of body fat accumulation in rats fed MCT.

Adipose Tissue↗
PubMed2002

Comparison of diet-induced thermogenesis of foods containing medium- versus long-chain triacylglycerols.

The purpose of this study was to investigate the effect of 5-10 g of medium-chain triacylglycerols (MCT) on diet-induced thermogenesis in healthy humans. The study compared diet-induced thermogenesis after ingestion of test foods containing MCT and long-chain triacylglycerols (LCT), using a double-blind, crossover design. Eight male and eight female subjects participated in study 1 and study 2, respectively. In both studies, the LCT was a blend of rapeseed oil and soybean oil. In study 1, the liquid meals contained 10 g MCT (10M), a mixture of 5 g MCT and 5 g LCT (5M5L), and 10 g LCT (10L). In study 2, the subjects were given a meal (sandwich and clear soup) with the mayonnaise or margarine containing 5 g of MCT or LCT. Postprandial energy expenditure was measured by indirect calorimetry before and during the 6 h after ingestion of the test meals. Diet-induced thermogenesis was significantly greater after 5M5L and 10M Ingestion as compared to 10L ingestion. Ingestion of the mayonnaise or margarine containing 5 g MCT caused significantly larger diet-induced thermogenesis as compared to that of LCT. These results suggest that, in healthy humans, the intake of 5-10 g of MCT causes larger diet-induced thermogenesis than that of LCT, irrespective of the form of meal containing the MCT.

Adult↗