Biomedical subjects

Ho-Seong Han

Publications and source records attributed to Ho-Seong Han.

At least 19 recordsLinked to original sources

PubMed2006

Total laparoscopic left lateral sectionectomy performed in a child with benign liver mass.

Despite the increasing number of recent reports on laparoscopic liver resection in adults, there are only rare reports on such a procedure being performed in children. The authors report here on a total laparoscopic left lateral sectionectomy that was performed in a 5-year-old girl who had a cystic tumor of the liver. The operative time was 150 minutes, the estimated blood loss was about 100 mL, and no intraoperative transfusion was required. The patient was discharged on postoperative day 11 without any significant complications. The postoperative pathology of the specimen confirmed it to be a mesenchymal hamartoma of the liver with a disease-free resection margin. This case demonstrates that laparoscopic liver resection can be a safe and feasible operative procedure for the pediatric patient with liver disease.

Child, Preschool↗

PubMed2006

The safety of a laparoscopic cholecystectomy in acute cholecystitis in high-risk patients older than sixty with stratification based on ASA score.

The purpose of this study was to evaluate the safety of a laparoscopic cholecystectomy (LC) for acute cholecystitis (AC) in patients older than sixty years of age, with stratification based on the ASA (American Society of Anesthesiologists) score. For five years, 137 patients older than sixty, who had undergone a LC for AC, were classified into three groups; ASA 1 (n = 33), ASA 2 (n = 79) and ASA 3 (n = 25). Preoperative percutaneous gallbladder drainage was performed in eight of the 137 cases (5.8%). All except one underwent one-stage management and 19.7% patients underwent emergency surgery within 24 hours of the index admission of AC. The preoperative hospital stay for ASA 3 (8.8 days) was longer than that for ASA 1 (5.6 days). There was a higher proportion of complicated cholecystitis and a longer operating time in ASA 2 (50.6%, 111 min.) and 3 (66.7 %, 114 min.) than in ASA 1 (24.2%, 85 min.) (p<0.05). Morbidity was more frequent in ASA 3 (20.0%) than in ASA 1 (9.1%). However, the open conversion rate, time to diet, and postoperative hospital stay were similar in the three groups (p>0.05). We conclude that a LC for AC may be an effective treatment option in elderly-high risk patients.

Age Factors↗

PubMed2006

Laparoscopic end-to-end choledochocholedochostomy for bile duct injury during laparoscopic cholecystectomy.

Bile duct injury is one of the serious complications associated with laparoscopic cholecystectomy. If the bile duct injury is circumferential or the bile duct is transected, conversion to open surgery is usually done. With the continuing development of laparoscopic techniques, procedures that previously seemed implausible can now be performed laparoscopically. We describe a case of successful laparoscopic end-to-end choledochocholedochostomy after the common bile duct was mistaken for the cystic duct intraoperatively and transected.

Cholecystectomy, Laparoscopic↗

PubMed2006

Total laparoscopic right posterior sectionectomy for hepatocellular carcinoma.

A 57-year-old man presented with a liver mass that had been detected on physical examination. The abdominal computed tomography scan revealed a 5-cm single nodular hepatoma located in segments 6 and 7. A total laparoscopic right posterior sectionectomy was performed for this lesion. The anatomical demarcation of the posterior section was possible with selective control of a Glissonian pedicle to that section. The patient was discharged on postoperative day 13 without complications. The postoperative pathology confirmed a hepatocellular carcinoma with a 1-cm free resection margin. The patient had no evidence of recurrence at 12-month follow-up. To our knowledge, this is the first reported case of total laparoscopic right posterior sectionectomy in segments 6 and 7.

Carcinoma, Hepatocellular↗

PubMed2006

Syngenic bone marrow cells restore hepatic function in carbon tetrachloride-induced mouse liver injury.

Progenitor cells in bone marrow have been explored for the treatment of liver injury. Stem cell homing to the injured tissue is regulated through stromal cell derived factor-1 (SDF-1) and its receptor CXCR4. We hypothesized that syngenic bone marrow cells (BMCs) would restore hepatic function in the injured liver through the regulation by SDF-1/CXCR4 system. After injecting carbon tetrachloride (CCl(4)), the mice were injected with syngenic BMCs or normal saline. Morphological and functional analysis of the liver was performed. Flow cytometry for the stem cell markers and CXCR4 was done with the liver, BM, and spleen cells from each group. Carboxyfluorescein diacetate succinimidyl ester was used to trace the homing of transplanted BMCs. The SDF-1 expression of the liver was assessed by immunohistochemistry. Hepatosplenomegaly and necrosis of the CCl(4)-injected mouse liver were improved after BMCs transplantation The hepatic enzymes were increased after injury and then decreased after BMCs transplantation. The expression of stem cell markers and CXCR4 was exclusively increased in the damaged liver compared to the BM and spleen, and even more elevated after BMCs transplantation. SDF-1 expression in the liver was observed after CCl(4) injection and it was elevated after BMCs transplantation. The intrinsic and extrinsic BMCs migrate specifically to the injured liver rather than BM or spleen, and the transplanted BMCs contribute to the repair of the damaged liver. SDF-1/CXCR-4 interaction plays a role in stem cell homing toward the damaged organ, and transplanted BMCs are involved in the up-regulated SDF-1 expression seen in the injured liver.

Actins↗

PubMed2006

Initial experiences with laparoscopy-assisted and total laparoscopy for anatomical liver resection: a preliminary study.

Although laparoscopic surgery has become more popular, its technical difficulties have limited the applications of this technique to liver surgery. We report here on our experience with liver resection with using the laparoscopy-assisted (Lap-Assist) and total laparoscopic (Total-Lap) methods. From April 2001 to June 2003, a total of 20 laparoscopic anatomical resections of the liver were retrospectively reviewed. These were comprised of 10 cases in which the Lap-Assist method was used (these were performed during the early study period), and 10 cases in which the Total-Lap was used (these were done in the later study period). In the Lap-Assist group, the following resections were performed: 7 cases of left lateral sectionectomy, a case of left hemihepatectomy, a case of right hemihepatectomy and a case of open conversion. In the Total-Lap group, 6 cases of left hemihepatectomy and 4 cases of left lateral sectionectomy were performed. The sizes of the incisions were 8.7 cm and 4.6 cm, respectively, (p=0.000). There were no differences in the operation times, the transfusion amounts, the starting days of the patients' diets, the complication rates or the durations of the hospital stay between the two groups. Both the laparoscopy-assisted method and the total laparoscopic method are feasible to use for performing anatomical liver resection.

Adult↗

PubMed2005

Nontraumatic perforation of the bile duct treated with laparoscopic surgery.

Nontraumatic perforation of the bile duct (NPB), which has also been reported as spontaneous perforation of the bile duct, is a disease in which spontaneous perforation occurs in the wall of the extrahepatic or intrahepatic duct without any traumatic or iatrogenic injury. Although NPB in adults is extremely rare, we have treated two such patients using a laparoscopic technique. Both cases presented with intra-abdominal abscesses associated with common bile duct stones, and laparoscopic surgery for those conditions was initially performed. During the procedure, NPB was identified in the right intrahepatic duct in one case and in the left intrahepatic duct in the other case. Laparoscopic surgery consisting of common bile duct exploration, T-tube placement, cholecystectomy, and abscess drainage was safely performed in both cases. Both patients eventually recovered without major complications. Although NBP is an uncommon disease, it can be treated successfully with a laparoscopic procedure.

Abdominal Abscess↗

PubMed2004

New operative method for fundal variceal bleeding: fundectomy with periesophagogastric devascularization.

Cirrhotic patients with gastric fundal bleeding occasionally require operative intervention. Fundal variceal bleeding may be controlled by performing a fundectomy in addition to periesophagogastric devascularization. For 23 patients with cirrhosis, preoperative nonsurgical intervention alone could not achieve definitive control of variceal bleeding from the gastric fundus, and periesophagogastric devascularization with the fundectomy--specifically resection of the varix-bearing lesion in the fundus--was performed. Direct inspection and palpation determined the area for the fundectomy, and resection was limited to the engorged varix-bearing area. The overall mortality rate was 26.1%. There were two postoperative deaths (18.2%) among 11 patients in the elective operation group and four deaths (33.3%) among 12 patients in the emergency operation group ( p > 0.05). The mortality rates among class A, B, and C patients, following the Child-Pugh classification for hepatic functional reserve, were 0% (0/4), 23.1% (3/13), and 50.0% (3/6), respectively ( p > 0.05). The patients who died had been transfused preoperatively with a mean of 13.0 +/- 10.5 units, and those who survived received a mean of 6.00 +/- 5.96 units ( p > 0.05). Patients who were successfully stabilized by preoperative endoscopic intervention had significantly lower mortality ( p < 0.001). During follow-up there was no recurrent bleeding from gastric varices, and there was only one case (4.35%) of hemorrhage from esophageal varices. Hence, periesophagogastric devascularization and fundectomy offers an alternative operative method for cirrhotic patients with variceal hemorrhaging from the gastric fundus.

Adult↗

PubMed2004

Nontraumatic perforation of the bile duct in adults.

HYPOTHESIS: Nontraumatic perforation of the bile duct in adults is rare, and the management of this condition should resolve the primary pathologic lesion. DESIGN: Retrospective analysis of 11 patients who were diagnosed as having nontraumatic perforation of the bile duct. SETTING: A public university medical center and a private university medical center. PATIENTS: Five men and 6 women (median age, 64 years) with nontraumatic perforation of the bile duct were treated between September 1993 and May 2003. INTERVENTIONS: Two patients with common bile duct (CBD) stones, who were initially diagnosed as having mediastinal abscess and subcapsular biloma, respectively, were treated by nonoperative management, ie, endoscopic sphincterotomy and percutaneous abscess drainage. The remaining 9 patients were treated surgically, which included an exploration of the CBD, placement of a T tube, and a liver resection. MAIN OUTCOME MEASURES: Initial manifestation, primary disease, perforation site, management, surgical morbidity, and mortality. RESULTS: All patients had acute abdominal pain, which was caused by intraabdominal abscess in 7 patients, diffuse bile peritonitis in 3, and subcapsular biloma in 1. Their primary diseases were CBD stones in 7 patients, intrahepatic duct stone in 2, a choledochal cyst in 1, and phytobezoar with food stuff in the CBD in 1. Perforations occurred at the left intrahepatic duct in 9 patients, the CBD in 1, and the cyst wall in 1. All patients recovered, except 1 patient who expired owing to multiorgan failure because the operation could not be performed in time. CONCLUSIONS: Nontraumatic perforation of the bile duct should be suspected if perihepatic abscess or peritonitis is combined with biliary stone disease. The management of nontraumatic perforation of the bile duct should include the eradication of the primary pathologic lesion and the control of abscess or peritonitis.

Adult↗

PubMed2004

Laparoscopic exploration of the common bile duct in a patient with situs inversus totalis.

Situs inversus totalis (SIT) is a rare anomaly that can present technical difficulties during laparoscopic surgery. We report the first case of a combination of SIT and choledocholithiasis in a patient who was treated successfully with a laparoscopic exploration of the common bile duct (CBD). The chest x-ray showed a right-sided heart. An abdominal ultrasound and computerized tomography scan diagnosed the CBD and gallbladder stones as well as the SIT, with the liver and gallbladder on the left side, and the spleen on the right. The surgical techniques were modified in a mirror image fashion in order to provide access to the left upper quadrant. The surgeon and the camera assistant stood on the patient's right side, and the first assistant stood on the left. The ports were introduced in a mirror image of the conventional setup. The SIT was confirmed using a telescope via an umbilical incision. A laparoscopic exploration of the CBD with a cholecystectomy was carried out successfully. The postoperative course was uneventful. This report shows that a CBD stone in a patient with SIT can be treated safely using laparoscopic surgery.

Cholecystectomy, Laparoscopic↗

PubMed2004

Laparoscopic Roux-en-Y choledochojejunostomy for benign biliary disease.

Roux-en-Y choledochojejunostomy (RYCJ) is frequently used for biliary bypass surgery. However, reports on laparoscopic RYCJ are scarce. Between February 1997 and February 2002, laparoscopic RYCJ was performed in 6 patients with a recurrent common bile duct (CBD) stone or a benign biliary stricture. The first procedure involved the laparoscopic preparation of the CBD. The Roux limb prepared with an endo-GIA was brought up near the proximal CBD. An end-to-side choledochojejunostomy was then performed using an intracorporeal suture. Jejunojejunostomy was performed using an endo-GIA. The mean operating time was 358.3 minutes. A postoperative complication occurred in 1 patient who had an episode of melena, which resolved spontaneously. All patients were free of symptoms during the follow-up period (27.5 months). Laparoscopic RYCJ may be a useful option, especially in the treatment of benign biliary disease.

Adult↗

PubMed2004

Laparoscopic treatment of intrahepatic duct stone.

Between October 1998 and November 1999, 12 patients with IHD stones, without severe ductal stricture, adhesion due to previous exploration, intrahepatic abscess or suspicious malignancy, were in the subjects of this study. A 4-port-technique was performed, with a 4-way flexible choledochoscope, via a choledochotomy, for removal of the stones. The postoperative follow-up for recurrent stones was performed with a cholangiogram and an ultrasonogram, and the quality of life was evaluated by a questionnaire for the symptoms. The mean operating time was 288.3 (150-420) minutes. There was only one postoperative complication, but no intraoperative transfusions or perioperative mortality. Remnant stones were detected in 1 patient on the final T-tube cholangiogram, which was removed by percutaneous choledochoscopy, through the tract matured by the T-tube. There were no cholangitis or recurrent stones during the mean follow-up period of 31.4 (10-45) months. With increasing demand for minimal invasiveness, the laparoscopic treatment of IHD stones could be an effective option.

Adult↗

PubMed2003

In vitro differentiation of natural killer T cells from human cord blood CD34+ cells.

Natural killer T (NKT) cells are involved in innate immune defence and also in the regulation of adaptive immune responses. However, the development of NKT cells in vitro has not been fully characterized and culture conditions have not been fully optimized. In the present study, we found that an NKT cell fraction developed during the in vitro culture of cord blood (CB) CD34+ cells, and this was subsequently characterized both phenotypically and morphologically. CD34+ cells purified from 10 human CB were cultured in the presence of several cytokines and analysed by flow cytometry, light microscopy and electron microscopy. The NKT cell fraction, defined phenotypically (CD3+CD16+CD56+CD94+) as expressing the invariant T-cell receptor Valpha24 and Vbeta11, appeared in the CD56hi fractions. Intracytoplasmic staining demonstrated that interferon-gamma and interleukin 4 (IL-4) were detected in the CD56hi fractions. IL-15 was essential and, in combination with either flt3-ligand (FL) or stem cell factor (SCF), was sufficient to induce the development of NKT cells. The phenotype of the NKT cell fraction was CD45RO+CD45RA- and CD4+CD8alpha+. Morphologically, they were very large, with either round or oval nuclei, moderately condensed chromatins, voluminous weakly basophilic cytoplasm and various cytoplasmic granules such as dense core granules, multivesicular bodies, and intermediate form granules. When CD34+ cells purified from bone marrow (BM) were compared with those from CB, the latter were consistently more efficient at generating CD56hi NKT cell fractions. In conclusion, IL-15 in combination with FL and/or SCF can induce the differentiation of NKT cells from human CB CD34+ cells.

Antigens, CD↗

PubMed2003

Laparoscopic wedge resection with handsewn closure for gastroduodenal tumors.

PURPOSE: To evaluate the feasibility and efficacy of laparoscopic wedge resection with handsewn closure in gastroduodenal tumors. METHODS: Laparoscopic wedge resection was performed in 16 patients with gastroduodenal tumor between May 2000 and December 2002. Every case, except one, was performed via an extragastric approach; a transgastric approach was adopted in a single case. Excision of the lesion was performed manually by means of electrocautery or ultrasonic coagulating shears and closed by manual intracorporeal running suture. RESULTS: Among the 16 cases, two cases were treated using a laparoscope-assisted method, but there was no case of conversion to open surgery. Mean size of lesions was 27.9 mm in diameter and the mean operation time was 219 minutes. In all cases, a complete tumor excision with negative surgical margins was obtained. The final pathologic diagnoses were ectopic pancreas (4 cases), gastrointestinal stromal tumor (3 cases), leiomyoma (2 cases), adenomyoma (2 cases), tubular adenoma (1 case), Brunner's gland hyperplasia (1 case), carcinoid tumor (1 case), eosinophilic granuloma (1 case), and post-endoscopic mucosectomy state for early gastric cancer (1 case). The average numbers of days to first postoperative oral food intake and hospital stay were 3.1 days and 6.0 days, respectively. There were no postoperative complications. CONCLUSIONS: Laparoscopic wedge resection with handsewn closure should be considered as a valid treatment option for selected gastroduodenal tumors in terms of feasibility, safety, and cost. A more efficient surgical instrument and technique should be developed in future.

Adolescent↗

PubMed2003

Hand-assisted laparoscopic total gastrectomy.

Although the standard method to manage gastric cancer is still radical gastrectomy, minimally invasive surgery is of great interest in early gastric cancer because of its potential impact on improving the quality of life, if the disease is curable. With its degree of technical difficulty, laparoscopic total gastrectomy has not yet met with widespread acceptance. However, using a hand-access device, a total gastrectomy and Roux-en-Y esophagojejunostomy with a D1 plus alpha lymph node dissection and omentectomy with an Ultrashear was performed in its entirety. The operation took 6 hours, and the blood loss was 500 mL. The patient recovered uneventfully and was discharged on the 16th postoperative day. In terms of recovery and quality of life, laparoscopic total gastrectomy is a technically feasible and reasonable option for the management of early gastric cancer in the proximal stomach, especially when an endoscopic mucosal resection is not indicated.

Adenocarcinoma↗

PubMed2003

Laparoscopy-assisted D2 subtotal gastrectomy in early gastric cancer.

The purpose of this study is to prove the safety and efficacy of laparoscopy-assisted subtotal gastrectomy and D2 lymph node dissection using 4 ports and an EEA stapler with a Billroth I anastomosis. From 1999 to 2001, 20 patients with EGC located in the distal stomach underwent laparoscopy-assisted Billroth I gastrectomy (LABIG). A 4-port-technique was performed for omentectomy, vascular ligation, and D2 lymph node dissection. A mini-incision was created between the two ports in the epigastric area and a gastroduodenal anastomosis with an EEA stapler and a distal resection was performed. The mean operating time was 261.8 (170-410) minutes. There was one postoperative complication without any intraoperative transfusions or perioperative mortality. The number of harvested nodes was 31.9 +/- 11.4. Mean distance from the lesion to the margin of resection was 5.3 +/- 2.2 cm proximally and 4.0 +/- 2.0 cm distally. On average, oral liquids were started at the 4.7th (3rd-8th) postoperative day. LABIG is a safe and effective way of performing D2 gastrectomy in terms of morbidity and oncological principles. A randomized controlled clinical study to compare long-term survival and quality of life is warranted.

Abdomen↗

PubMed2003

Serum cytokine profiles in patients with Plasmodium vivax malaria: a comparison between those who presented with and without hyperpyrexia.

Serum cytokine profiles in patients with Plasmodium vivax malaria who presented with and without hyperpyrexia were compared by a retrospective review of the medical records of the consecutive patients seen at the military hospitals near the demilitarized zone in the Republic of Korea from April 2000 through October 2001. Of 162 male patients studied, 120 (86.4%) presented with hyperpyrexia (i.e., an axillary temperature > or = 40 degrees C). The mean +/- SEM ages of the patients with and without hyperpyrexia were 21.5 +/- 0.14 and 21.9 +/- 0.39 years, respectively (P = 0.33). The mean +/- SEM concentrations of serum interleukin (IL)-6 (379.7 +/- 44.1 pg/mL versus 105.4 +/- 26.8 pg/mL; P = 0.002), IL-10 (583.4 +/- 58.2 pg/mL versus 142.4 +/- 39.7 pg/mL; P = 0.0001), and interferon-gamma (312.6 +/- 33.9 pg/mL versus 112.9 +/- 27.1 pg/mL; P = 0.0001) were significantly higher in patients with hyperpyrexia compared with those without hyperpyrexia. The mean +/- SEM concentrations of serum tumor necrosis factor-alpha were 155.5 +/- 54.5 pg/mL and 109.9 +/- 29.3 pg/mL (P = 0.27) in patients who presented with and without hyperpyrexia, respectively. Further studies are needed to examine whether serum concentrations of these cytokines also parallel their concentrations at the tissue sites of their production and action.

Adult↗

PubMedDate not supplied

Evaluation of preoperative sonography in acute cholecystitis to predict technical difficulties during laparoscopic cholecystectomy.

PURPOSE: The aim of this study was to evaluate the role of preoperative sonography in predicting technical difficulties during laparoscopic cholecystectomy in patients with acute cholecystitis. METHODS: Sonographic assessment of 14 parameters was performed in 55 patients during a 9-month period: volume of gallbladder (GB), thickness of GB wall, pattern of GB wall thickening, size of largest gallstone, gallstone mobility, adhesion of GB to its bed, fat plane between GB and hepatoduodenal ligament, free fluid in GB fossa, common bile duct (CBD) dilatation, CBD stone(s), color and power Doppler signals in GB wall, and increased color and power Doppler signals in adjacent liver. Each of the 5 operative steps of laparoscopic cholecystectomy was scored as being difficult (1) or not (0). The scores for each step were added to obtain the overall difficulty score (0-5). We evaluated prospectively whether there were significant associations among the preoperative sonographic findings and the overall difficulty score, scores for each of the 5 operative steps, and operation time. RESULTS: The overall difficulty score was significantly associated with a GB volume of 50 cm(3) or more, GB wall thickness of 3 mm or more, and presence of color Doppler signals in the GB wall. Increased GB volume also made dissection of adhesions from the GB and dissection of Calot's triangle more difficult. Extraction of the GB from the abdomen was more difficult with a thickened GB wall or adhesion of the GB to its bed. The presence of a CBD stone, dilatation of the CBD (> or = 8 mm), color Doppler signals in the GB wall, and increased power Doppler signals in the adjacent liver were significantly associated with increased operation time. CONCLUSIONS: Based on our experience, preoperative determination of GB volume, GB wall thickness, and presence of color Doppler signals in the GB wall in patients with acute cholecystitis helps predict technical difficulties during laparoscopic cholecystectomy.

Adult↗