[Association between the genetic polymorphism of chemokine genes and asthma in Chinese children].
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Adolescent↗
Biomedical subjects
Hong Chen
Publications and source records attributed to Hong Chen.
[Clinical observation of cryptosporidiosis with intestinal bacterial co-infection].
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Bacterial Infections↗
[Treatment of Hirschsprung disease by botulinum toxin A through anorectal injection].
OBJECTIVE: To investigate the feasibility and short- term effect of injecting botulinum toxin A through anorectum to treat Hirschsprung disease. METHODS: Eight cases with Hirschsprung disease were proven pathologically or diagnosed by barium enema. At the clock point of 3,6,9 under supine posture,a total dose of 1.5 U/ kg of botulinum toxin A was injected into the internal anal sphincter and the submucosa of rectum. The pressure of anus- rectum was measured before operation and 1 month,1 year after operation,respectively. Barium enema examination was carried out within 3-6 months after operation,then once a year during followed- up. RESULTS: All of the patients recovered well without operative or postoperative complications. In the first year,all patients could defecate spontaneously without constipation and abdominal distention. Three cases had constipation again and slight abdominal distention 1 year after operation,but the symptoms were relieved after anus dilatation. The resting pressure of anus- rectum was lower 1 month after operation than that before operation (P< 0.01) in 8 cases,and still lower 1 year after operation (P< 0.05)in 6 cases. Barium enema examination within 3-6 months after operation revealed that the dilated colon shrank. CONCLUSION: Botulinum toxin A injection is a new,feasible and safe method for Hirschsprung disease,but the long- term efficacy needs to be further studied.
Administration, Rectal↗
[Association of mutations of HFE gene and hepatocellular carcinoma following chronic hepatitis B].
OBJECTIVE: To investigate the frequency of HFE gene variants in patients with hepatocellular carcinoma following chronic hepatitis B and to analyze their relationships. METHODS: 56 patients with hepatocellular carcinoma following chronic hepatitis B (HCC group) and 60 healthy blood donors (control group) were studied for the amino acid dimorphism at codon 63 (His63Asp=H63D) and codon 282 (Cys282Tyr = C282Y) of the HFE gene. The codon 63 and 282 dimorphisms were defined by PCR amplification of genomic DNA samples and restriction enzyme digestion using RsaI for C282Y and BclI for H63D. The association between hepatocellular carcinoma following chronic hepatitis B and HFE mutations were analyzed by Chi-square test. RESULTS: The genotype frequency of C2/C2 in the HCC group was markedly higher than that in the normal control group (10.7% vs 0) and there was a significant correlation between them. At the same time, the allele frequency of C2 in the HCC group was markedly higher than that in the normal control group (16.1% vs 1.7%) and there was a significant correlation between them also. CONCLUSION: The mutation of C282Y may be related with susceptibility to HCC after chronic hepatitis B. This outcome suggests that host HFE mutation may be an important factor related to the pathogenesis of HCC.
Adult↗
[Intermediate-term and long-term clinical evaluation of the Ahmed glaucoma valve implantation].
OBJECTIVE: To evaluate the Intermediate-term and long-term clinical results of Ahmed glaucoma valve (AGV) implantation in refractory glaucoma. METHODS: A retrospective study was conducted to document 221 eyes from 206 patients with refractory glaucoma who undergone AGV implantation or combined AGV implantation with other intraocular surgery. The subject had a minimum 6 months of follow-up after surgery. According to the successful definition of AGV implantation, life table's analysis was used to assess the cumulative probabilities of success in different follow-up time. Cox regression analysis was performed to estimate the relationships between survival outcomes and multiple predictors. RESULTS: The follow-up ranged from 6.03 to 86.30 months (mean 36.86 months). The cumulative probabilities of success at 0.5, 1, 2, 3, and 4 years were (88.5 +/- 2.2)%, (85.0 +/- 2.5)%, (76.4 +/- 3.1)%, (66.2 +/- 3.7)%, (55.5 +/- 4.7)% and of total success at 0.5, 1, 2, 3, and 4 years were (91.2 +/- 1.9)%, (88.6 +/- 2.2)%, (88.6 +/- 2.2)%, (86.8 +/- 2.5)%, (86.8 +/- 2.5)%, respectively. Intraocular pressure was reduced from a preoperative mean of (44.59 +/- 13.04) mm Hg (1 mm Hg = 0.133 kPa) to a postoperative mean of (16.52 +/- 4.61) mm Hg (P < 0.01). The early major complications included transient hypotony (19.5%), shallow anterior chamber (14.5%), tube blockage (11.3%), hemorrhage (7.2%). The late major complications were encapsulated bleb (10.9%), exposure of tube (5.0%), tube malposition (4.5%), corneal decompensation (2.3%), extrusion of implant (1.4%). CONCLUSIONS: AGV or combined AGV with other intraocular surgery is effective and relatively safe method for treating refractory glaucoma. The rates of success can be increased by means of effective management of the complications.
Adolescent↗
[Cytoplasmic expression of VP1 gene of coxsackievirus B3].
OBJECTIVE: To increase the immune effect of gene vaccine, T7 RNA polymerase was used to establish a system of cytoplasmic expression. METHODS: (1) The plasmid pT7 EMCVP1, including T7 promoter sequence, 5'-untranslated sequence of encephalomyocarditis (EMC) virus, VP1 sequence of coxsackievirus B3 (CVB3), was cotransfected with the plasmid pAR 3132, which codes for the T7 RNA polymerase, into HeLa cells and murine peritoneal macrophages. (2) The plasmid pT7 EMCVP1 and pAR 3132 were respectively transformed into the attenuated Salmonella typhimurium SL 7207. The two kinds of transformed bacteria were coinfected into murine peritoneal macrophages. RESULTS: (1) The target antigen VP1 in the cytoplasm was about 2-4-fold higher than that of pcDNA3 VP1 singly transfected. (2) After the murine peritoneal macrophages were coinfected by two kinds of transformed bacteria, the target antigen VP1 could also be detected. CONCLUSION: The pT7 EMCVP1 and pAR 3132 could be expressed in the cytoplasm of HeLa cells and murine peritoneal macrophages and the amount of the antigen VP1 increased remarkably as compared with that of pcDNA3 VP1 singly transfected.
Animals↗
[The effect of CSN1 S2, CSN3 and beta-lg genes on milk performance in Xinong Saanen dairy goat].
PCR-RFLP technique was applied to analyze correlation between the polymorphisms of CSN1 S2 (alpha(s2) casein), CSN3 (kappa casein) and beta-lg (beta-lactoglobulin) genes and milk performance in 69 individuals of Xinong Saanen dairy goat. The results showed that there was significant correlation between different genotypes of CSN1 S2 locus and milk yield:average milk yield of individuals with genotype FF was less than that of genotype NN (P < 0.05); the second lactation milk yield of individuals with genotype NN was over 90 kg higher than that with genotype FF (P < 0.01). This indicates that allele F of CSN1 S2 locus probably has significant negative effect on milk yield. The results of CSN3 gene digested with endonuclease Hind III cleavage showed that no significant difference of milk yield between genotype DE and genotype EE was detected in first, second, third and fourth lactation milk yield and average milk yield (P > 0.05). The results of CSN3 gene with endonuclease Taq I cleavage showed that no significant difference of milk yield among individuals with genotype TT, TC and CC was detected (P > 0.05). No polymorphism was detected in PCR products of CSN3 gene digested with endonuclease Hae III. The analysis of beta-lg gene's 5' flanking region (710 bp) by PCR-RFLP in Xinong Saanen dairy goat showed that milk yield of individuals with genotype AA was higher than that with genotype AB in second, third lactation milk yield and average milk yield (P < 0.05). The results implied that allele A of beta-lg gene's 5' flanking region is probably related to high milk yield.
5' Flanking Region↗
[The synthesis and fluorescence properties of doping europium-benzoic acid-phenanthroline complexes].
Eu1-x Lnx (BA)3Phen(Ln: Gd, La, Y; BA: benzoic acid; Phen: phenanthroline; x = 0.0-0.9) complexes are synthesized, and their similar structures showed by IR spectra. Fluorescence spectra indicate that the complexes can emit intense characteristic fluorescence from europium ion and the doping elements can enhance the fluorescence intensities of central europium ion and have co-fluorescence effect.
Benzoic Acid↗
[Phylogenesis analysis of 10 chinese indigenous pig populations].
Genetic relationship of 10 indigenous pig populations was analyzed by 10 microsatellite markers. Results showed that different phylogenetic methods led to different results. The NJ phylogenetic tree from Nei's genetic distance was consistent with the facts. The 10 pig populations were divided into 3 clusters. The first cluster was Guizhou miniature pig and its inbreeding lines, Diannan xiaoer and Wuzhishan miniature pig, the second Yimeng black pig and Rongchang pig, and the third Erhualian, Jinhua and Bama miniature pig.
Algorithms↗
Identification of differential genes in ovarian cancer using representational difference analysis of cDNA.
OBJECTIVE: To identify differential genes between normal ovarian epithelium tissue and ovarian epithelial cancer using representational difference analysis of cDNA (cDNA-RDA). METHODS: cDNA-RDA was performed to identify the differentially expressed sequences between cDNAs from cancer tissue and cDNAs from normal ovarian tissue in the same patient who was in the early stage of ovarian serous cystadenocarcinoma. These differentially expressed fragments were cloned and analyzed, then sequenced and compared with known genes. RESULTS: Three differentially expressed cDNA fragments were isolated using cDNA from normal ovarian tissue as tester and cDNA from cancer tissue as driver amplicon by cDNA-RDA. DP III-1 and DP III-2 cDNA clone showed significant homology to the cDNA of alpha actin gene; DP III-3 cDNA clone showed significant homology to the cDNA of transgelin gene. CONCLUSION: cDNA-RDA can be used to sensitively identify the differentially expressed genes in ovarian serous cystadenocarcinoma. Ovarian serous cystadenocarcinoma involves alteration of multiple genes.
Actins↗
Aberrant methylation: common in thymic carcinomas, rare in thymomas.
Thymic carcinoma, which is a rare epithelial neoplasm of the thymus gland, is different from thymoma in its clinical and pathological features. To clarify the mechanism underlying the aggressive behavior of thymic carcinoma, we examined the clinicopathologic features, aberrant methylation patterns of the tumor suppressor genes, and epidermal growth factor receptor (EGFRs) mutation in both thymic carcinomas and thymomas. Clinical data of 11 thymic cancers and 13 thymomas were reviewed. Resected samples of 5 thymic cancers and 6 thymomas selected from 24 cases were used for methylation and mutation studies. Positive tumor markers were more frequent in thymic cancers than in thymomas (p=0.0233), and the methylation index, which reflects the overall methylation pattern, was significantly higher in thymic carcinomas (p=0.0053). No tumors showed a mutation of EGFR, KRAS, and HER2. Thymic carcinoma is distinct from thymoma not only with respect to clinicopathological features, but also aberrant methylation patterns of the tumor suppressor genes.
Adult↗
[Clinical observation on abdominal cluster-needling combined with sacro-iliac-needling for treatment of chronic pelvic inflammation].
OBJECTIVE: To observe therapeutic effect of abdominal cluster-needling combined with sacro-iliac-needling on chronic pelvic inflammation. METHODS: One hundred and ten cases of pelvic inflammation were randomly divided into a treatment group and a control group. The treatment group of 70 cases were treated by abdominal cluster-needling combined with sacro-iliac-needling; the control group of 40 cases were treated by oral administration of Fuyankang tablet. Their therapeutic effects were compared. RESULTS: The cured rate and the total effective rate were 75.7% and 97.1% in the treatment group, and 37.5% and 85.0% in the control group, respectively, with significant difference between the two groups (P < 0.01, P < 0.05). CONCLUSION: The therapeutic effect of abdominal cluster-needling combined with sacro-iliac-needling is significantly better than that in the control group for chronic pelvic inflammation.
Abdomen↗
[Effects of pricking blood therapy on ACTH contents in the hypothalamus-pituitary-adrenal axis in the rat of experimental allergic rhinitis].
OBJECTIVE: To explore the mechanism of pricking blood therapy (PBT) for allergic rhinitis (AR). METHODS: The model rat of experimental allergic rhinitis (EAR) was established by administration of ovoglohulin and they were divided into five groups randomly: a model group, a PBT group, a TCM point application group, a beclomethasone dipropionate (BDP) treatment group, and a health control group. Adrenocorticotrophic hormone (ACTH) contents in hypothalamus, pituitary gland, adrenal gland and plasma were determined by radioimmunoassay before and after treatment. RESULTS: The ACTH levels in the hypothalamus, pituitary gland, adrenal gland and plasma in the rat of EAR were lower than those in the health control group (P < 0.01). After treatment of pricking blood ther, ACTH levels in the above tissues increased significantly (P < 0.01), with very significant differences (P < 0.01) as compared with the model group, similar to those in the BDP group (P > 0.05). CONCLUSION: Pricking blood therapy has a regulatory action on ACTH in HPA axis of rats with experimental allergic rhinitis.
Adrenocorticotropic Hormone↗
[Detection of fetal short tandem repeat genotype in maternal plasma by multiplex fluorescent polymerase chain reaction].
OBJECTIVE: To study the feasibility of using free fetal DNA in maternal plasma for non-invasive prenatal diagnosis. METHODS: Maternal DNA extracted from plasma samples of 10 pregnant women at early pregnancy, medium pregnancy and late pregnancy and their husband's DNA isolated from whole blood samples were used to detect genotype by multiplex fluorescent PCR at nine different polymorphic short tandem repeat(STR) loci. RESULTS: Fetus-specific alleles were found in maternal plasma samples studied. By the application of these polymorphic short repeat sequences, every pregnant women/husband pair was informative in at least three of nine loci. Paternally inherited fetal alleles were detected in 23 of 30 plasma samples. They are 6/10 cases in early pregnancy, 8/10 cases in middle pregnancy and 9/10 cases in late pregnancy respectively. CONCLUSION: Fluorescent multiplex PCR can be used for amplification of male and female fetal STRs in maternal plasma to obtain genetic information, which may have implication for non-invasive prenatal diagnosis of certain hereditary diseases independent of the fetal sex.
Adult↗
[Research on the genetic polymorphism in crested ibis by RMAPD].
The genetic polymorphism of Yangxian artificially reared 43 crested ibises (Nipponia nippon) was firstly investigated by RMAPD (random microsatellite amplify polymorphic DNA) marker. The results showed that RMAPD was more stable and polymorphic than RAPD. 2147 bands were amplified by 12 pairs of primers,of which 93 bands had polymorphisms. The band sharing frequency and the genetic diversity index were 0.718 and 3.664, respectively, indicating that the genetic structure of crested ibis population was simple and poor genetic variations existed in crested ibis population in Yangxian. It is imperious to protect the genetic diversity of crested ibis population.
Animals↗
[Determination of the related substances in tirapazamine by high performance liquid chromatography].
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Chromatography, High Pressure Liquid↗
Population genetic structure of Anopheles gambiae mosquitoes on Lake Victoria islands, west Kenya.
BACKGROUND: Understanding the genetic structure of island Anopheles gambiae populations is important for the current tactics in mosquito control and for the proposed strategy using genetically-modified mosquitoes (GMM). Genetically-isolated mosquito populations on islands are a potential site for testing GMM. The objective of this study was to determine the genetic structure of A. gambiae populations on the islands in Lake Victoria, western Kenya. METHODS: The genetic diversity and the population genetic structures of 13 A. gambiae populations from five islands on Lake Victoria and six villages from the surrounding mainland area in the Suba District were examined using six microsatellite markers. The distance range of sampling sites varied between 2.5 and 35.1 km. RESULTS: A similar level of genetic diversity between island mosquito populations and adjacent mainland populations was found. The average number of alleles per locus was 7.3 for the island populations and 6.8 for the mainland populations. The average observed heterozygosity was 0.32 and 0.28 for the island and mainland populations, respectively. A low but statistically significant genetic structure was detected among the island populations (FST = 0.019) and between the island and mainland populations (FST = 0.003). A total of 12 private alleles were found, and nine of them were from the island populations. CONCLUSION: A level of genetic differentiation between the island and mainland populations was found. Large extent of gene flow between the island and mainland mosquito populations may result from wind- or human-assisted dispersal. Should the islands on Lake Victoria be used as a trial site for the release program of GMM, mosquito dispersal between the islands and between the island and the mainland should be vigorously monitored.
Alleles↗
Superluminal pulse reflection and transmission in a slab system doped with dispersive materials.
The reflection and transmission of a pulse through a slab which is doped with two-level or three-level atoms are investigated theoretically. The doped atoms can be passive (absorptive) or active (gain). We find that both the reflected and transmitted pulses can be superluminal simultaneously for the slab doped with absorptive two-level atoms at the slab thickness equal to (2m+1) lambda(0) /4 sqrt[epsilon(b)] (where lambda(0) is the center wavelength of the incident pulse, and epsilon(b) is the background dielectric constant of the slab) or with active three-level atoms at any thickness. By adjusting the thickness or background dielectric constant of the slab, the reflected pulse can be controlled from superluminal to subluminal or vice versa for the slab doped with absorptive two-level or absorptive three-level atoms. The energy percentage in the reflected pulse can also be controlled by changing the thickness of the slab, and the doped atoms.
Journal Article↗