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Hong-Hui Lin

Publications and source records attributed to Hong-Hui Lin.

10 recordsLinked to original sources
PubMed2006

Chiral multinuclear macrocyclic polyamine complexes: synthesis, characterization and their interaction with plasmid DNA.

A series of multinuclear macrocyclic polyamine metal (Zn(2+), Cu(2+), Co(2+)) complexes containing chiral dipeptide linkage were synthesized and used as artificial nuclease enzyme model. The interaction between the complexes and plasmid DNA (pUC19) was studied, and the results revealed that these complexes could act as powerful catalysts for the cleavage of plasmid DNA under physiological conditions.

Catalysis↗
PubMed2006

Synthesis and DNA cleavage activities of mononuclear macrocyclic polyamine zinc(II), copper(II), cobalt(II) complexes which linked with uracil.

Mononuclear macrocyclic polyamine zinc(II), copper(II), cobalt(II) complexes, which could attach to peptide nucleic acid (PNA), were synthesized as DNA cleavage agents. The structures of these new mononuclear complexes were identified by MS and (1)H NMR spectroscopy. The catalytic activities on DNA cleavage of these mononuclear complexes with different central metals were subsequently studied, which showed that copper complex was better catalyst in the DNA cleavage process than zinc and cobalt complexes. The effects of reaction time, concentration of complexes were also investigated. The results indicated that the copper(II) complexes could catalyze the cleavage of supercoiled DNA (pUC 19 plasmid DNA) (Form I) under physiological conditions to produce selectively nicked DNA (Form II, no Form III produced) with high yields. The mechanism of the cleavage process was also studied.

Cobalt↗
PubMed2006

The conjugates of uracil-cyclen Zn(II) complexes: synthesis, characterization, and their interaction with plasmid DNA.

As an important nucleobase in RNA, uracil was introduced into the side chain of cyclen (1,4,7,10-tetraazacyclododecane) by using phenylene dimethylene group as bridge. The target compounds 5 were obtained in high yields. Subsequent experiments demonstrated that the uracil-cyclen conjugates can bind Zn(2+) cation rapidly in water, and the catalytic activities of their Zn(II) complexes 6 in DNA cleavage were also studied. The results showed that Zn(II) complexes can catalyze the cleavage of supercoiled DNA (pUC 19 plasmid DNA) (Form I) to produce nicked DNA (Form II and Form III) with high selectivity. In water solution, complex 6b may form a unique and stable supramolecular structure, which benefits the DNA cleavage process.

Base Sequence↗
PubMed2006

The oxidative damage of plasmid DNA by ascorbic acid derivatives in vitro: the first research on the relationship between the structure of ascorbic acid and the oxidative damage of plasmid DNA.

To study the structure-function relationship of the oxidative-damage effect of ascorbic acid, we have focused on the interaction between plasmid DNA pUC19 and a series of ascorbic acid derivatives modified on different OH groups in the presence of transition metal ions. Some ascorbic acid derivatives can selectively cleave plasmid DNA from Form I to Form II in the presence of low concentration of Cu2+ just like ascorbic acid itself, while other derivatives oxidatively damage plasmid DNA slightly. We found that those derivatives with unattached 2-OH and 3-OH groups retain the ability to cleave the plasmid DNA. The derivatives that have been methylated on 2-OH or 3-OH can only cleave plasmid DNA softly, and those derivatives that have been protected on both 2-OH and 3-OH can hardly exert an oxidative damage on plasmid DNA under the same condition. Form these results, we can draw the conclusion that 2-OH and 3-OH groups of the ascorbic acid molecule contribute most to this biological activity.

Ascorbic Acid↗
PubMed2006

[Construction of infectious cDNA clone of cucumber mosaic virus satellite RNA XJs1 and preliminary study on its biological function].

Cucumber mosaic virus (CMV) sugar beet isolate caused yellow mosaic, leaf distortion, crinkle and stunt symptoms on sugar beet in nature. It exhibited some special biological properties with narrower host range and had no symptom on Nicotiana glutinosa L. and Nicotiana tobacum L. cv. NC-89. A new satellite RNA, XJs1 was found to be associated with the helper virus. In order to know the cause of the special pathogenicity of the CMV isolate. Full-length infectious cDNA clone of CMV satellite RNA XJs1, pMSC20, was constructed by reverse transcriptase-polymerase chain reaction (RT-PCR). Sequence analysis showed that the satellite RNA consists of 384 nucleotides (nt) (GenBank accession number: D0070748). Compared the nucleotide sequence of satellite RNA XJsl with those of other representative CMV satellite RNAs displayed that it contains typical necrogenic consensus sequence block from positions 325 to 350, and shared 73.27% - 91.93% nucleotide sequence identity with some published CMV satellite RNAs. By in vitro transcription, satellite RNA XJsl was inoculated on Nicotiana tabacum and Nicotiana glutinosa together with CMV-AH, a CMV isolate without satellite RNA. The results showed that satellite RNA XJsl could attenuate symptoms on Nicotiana tabacum and Nicotiana glutinosa induced by CMV-AH. Detection by RT-PCR and Northern blot hybridization revealed XJs1 obtained replication in the above two host plants, showing the pathogenicity changes of CMV-AH on Nicotiana tabacum and Nicotiana glutinosa were induced by co-infecting with satellite RNA XJsl. These results indicated that XJsl is probably an attenuate satellite RNA. The relationship between helper virus, satellite RNA and host plants is discussed.

Cucumber Mosaic Virus Satellite↗
PubMed2005

Dinuclear macrocyclic polyamine zinc(II) complexes linked with flexible spacers: synthesis, characterization, and DNA cleavage.

Dinuclear macrocyclic polyamine zinc(II) complexes, which have two cyclen groups linked by flexible spacers, have been synthesized as DNA cleavage agents. The structures of these new dinuclear complexes are consistent with the data obtained from elemental analysis, MS and 1H NMR spectroscopy. The catalytic activity of these dinuclear complexes on DNA cleavage was studied. The results showed that the dinuclear zinc(II) complexes can catalyze the cleavage of supercoiled DNA (pUC 19 plasmid DNA) (Form I) under physiological conditions to produce selectively nicked DNA (Form II).

DNA↗
PubMed1998

Studies on Light Harvesting Complexes of Photosystem II in a Chlorophyll-deficient Barley Mutant NYB.

As compared with wild type barley, the LHCII content of NYB barley mutant reduced markedly and its polypeptide components also changed: the contents of 24 kD, 27 kD and 30 kD polypeptides reduced and 26 kD polypeptide was missing. Northern blot analysis with specific cab gene probe showed that there was no difference in the LHCII mRNA level between the wild and mutant barley, indicating the decreased LHCII level in mutant barley was not due to a reduced transcription or accumulation of its mRNA. The rudimentary development state of thylakoids in NYB barley may be correlated with the loss of 26 kD polypeptide.

Journal Article↗
PubMed1996

Purification and Characterization of a DTT-sensitive Protease Associated with PS II Particles.

A DTT-sensitive protease was purified by hydrophobic chromatography on butyl-Toyopearl 650 M and anion-exchange chromatography on DEAE-Sephadex A-50 from the 1 M NaCl extract of PS II particles. The results of SDS-PAGE and gel-filtration chromatography on Superose 12 Have showed that it is a monomeric protease with a MW of 37 000. The protease generated polypeptides of 13.2 kD, 12 kD and 10.5 kD fragments from 18 kD protein, and those of 23 kD, 22 kD and 20 kD fragments from 24 kD protein, respectively. The protease had maximum activity at pH 8.0, and showed to be sensitive to DTT and beta-Me but insensitive to the ionic strength of NaCl. Studies with protease inhibitors suggested that this enzyme is not a serine-protease.

Journal Article↗
PubMedDate not supplied

Salicylic acid decreases the levels of dehydrin-like proteins in Tibetan hulless barley leaves under water stress.

The effects of salicylic acid (SA) on the accumulation of dehydrins in leaves of Tibetan hulless barley seedlings under water stress were investigated. The results indicated that SA decreased the levels of the four dehydrin-like proteins induced by water stress. The concentrations of these dehydrin-like proteins increased under water stress. However, their levels in SA-pretreated seedlings were always lower than in those receiving only water stress. Our results also indicated that the levels of dehydrin-like proteins decreased as the SA concentration increased. In SA-pretreated seedlings, electrolyte leakage, MDA and H2O2 content were rather higher than in seedlings receiving only water stress. By these results, we suggest that lower levels of dehydrin-like proteins in seedlings with SA treatment may be due to the greater accumulation of H2O2 induced by SA, which causes more oxidative injury under water stress.

Electrolytes↗