Biomedical subjects

Hong-bing Jiang

Publications and source records attributed to Hong-bing Jiang.

4 recordsLinked to original sources

PubMed2005

[In vitro study on cranial neural crest differentiating into ectomesenchymal cell of the first branchial arch by FGF-8].

OBJECTIVE: To investigate the effects of FGF-8 on cranial neural crest cell (CNCC) differentiating into ectomesenchymal cell of the first branchial arch, and determine the appropriate dose and stage of CNCC exposure to FGF-8. METHODS: Cranial neural crest explants were cultured in free-serum medium containing modified DMEM/F12 and different doses of FGF-8. The differentiation type of CNCC were determined by in situ hybridization for Hoxa2 and immunocytochemistry for vimentin. RESULTS: Pre-emigrating CNCC demonstrated the negative Hoxa2 stain and positive vimentin stain after treated by 100 ug/FGF-8. Both post-emigrating CNCC group and control group were positive for Hoxa2 and vimentin stain. CONCLUSIONS: On the early stage of CNCC emigration, the first branchial arch phenotype of CNCC could be induced by FGF-8. This experiment could provide in vitro model for study on the mechanism of tooth-jaw regeneration.

Animals↗

PubMed2004

[The correlation between neck lymphy node metastasis and matrixmetalloproteinase-2 expression at the invasive tumor front of oral squamous cell carcinomas].

OBJECTIVE: To explore the correlation between neck lymph node metastasis and matrix metalloproteinase-2 expression at the invasive tumor front of oral squamous cell carcinomas(OSCC). METHODS: Immunohistochemistry LsAB technique was used to observe the expression of MMP-2 at the invasive tumor front and center of OSCC, and the correlation between the expression of MMP-2 in OSCC and neck lymph node metastasis were respectively analyzed by statistics. RESULTS: The results demonstrated that MMP-2 existed in all 71 cases, which the expression of MMP-2 at the OSCC front was more significant than that of MMP-2 at the OSCC center (P < 0.01), and related to neck lymph node metastasis (P < 0.05). CONCLUSION: The expression of MMP-2 at the OSCC front could be considered as an index of judging the present of neck lymph node metastasis of OSCC.

Adult↗

PubMed2004

[In vitro culture and biological characteristics of cranial neural crest stem cell].

OBJECTIVE: According to development biology, cranial neural crest stem cell (CNCSC) can differentiate into precursor cells of tooth, jaw and peripheral nerve system, but in vitro study is less reported. In the present study, CNCSC were dissociated and cultured in vitro, and the biological characteristics of CNCSC was investigated. METHODS: Cranial neural tubes, dissected from mouse E9d, were explanted onto fibronectin-coated dishes. CNCSCs emigrated from the explanted neural tubes, cultured in a serum-free medium containing modified DMEM/F12. Biological characteristics of CNCSC were detected by morphology, nuclear labeled with BurdU and immunocytochemistry. RESULTS: Fibroblast-like CNCSCs demonstrated the characteristics of stem cell, such as clonality, self-renewal and multipotentiality. The result of immunocytochemical stain showed that CNCSC expressed HNK-1 antigen. CONCLUSION: CNCSC were cultured successfully, providing a experimental basis for study on tooth/jaw-like differentiation of CNCSC in vitro, especially providing a cell source for investigating tooth/jaw regeneration.

Animals↗

PubMed2002

[The development of an automatic microscopic urinary sediment analyzer].

This paper introduces an automatic microscopic urinary sediment analyzer. A set of basic algorithms, including pretreatment and picking up characteristics is brought forward to realize the automatic segmentation of urinary sediment. This paper also characterizes the visible components in urinary sediment in morphology and has sum up 7 morphology parameters and a simple method of classification.

Algorithms↗