Biomedical subjects

Hongbo Zhou

Publications and source records attributed to Hongbo Zhou.

4 recordsLinked to original sources

PubMed2006

Simple pulse compression scheme based on filtering self-phase modulation-broadened spectrum and its application in an optical time-division multiplexing system.

We propose a simple and stable pulse compression scheme based on a filtering self-phase modulation broadened spectrum in a highly nonlinear fiber. It is found experimentally that under offset filtering the compressed pulse has a better quality compared with center filtering, such as a no pulse pedestal. Furthermore, numerical simulations reveal that the scheme has the potential to improve the pulse extinction ratio during compression. Finally, based on the obtained pulse train the 4 x 10 Gbits/s optical time-division multiplexing system is studied for verification of the high pulse quality. The compression scheme has the advantages of simple configuration, being pedestal-free, and having a high pulse extinction ratio.

Journal Article↗

PubMed2006

Genome-sequence analysis of the pathogenic H5N1 avian influenza A virus isolated in China in 2004.

Analysis of the sequences of the genome of the avian influenza A/chicken/Hubei/327/2004 (H5N1) virus, isolated from a poultry farm during the outbreak of avian influenza (AI) in Hubei Province, central China, in the spring of 2004, revealed that the hemagglutinin (HA) gene of the virus was genetically similar to those of the H5 highly pathogenic avian influenza virus (HPAI). Notably, the neuraminidase gene of the virus had a 20-amino acid deletion in the stalk region and a 5-amino acid deletion in the NS gene which belonged to allele B. Furthermore, the internal genes (PB2, PA, NP, M2) of the A/chicken/Hubei/327/2004 virus with the particular amino acid residues were more closely related to H5N1 viruses of 2000-2003 isolated in Hong Kong and the AIV of Thailand and Vietnam in 2004, but less likely to evolve from the viruses of Hong Kong 1997. Finally, our results demonstrated that the influenza A/chicken/Hubei/327/2004 (H5N1) virus was similar to those of the AI viruses isolated from Hong Kong (2000-2003), Vietnam, and Thailand rather than the viruses from the 1997 lineage of Hong Kong and with closest genetic relatives to the influenza A/Chicken/Hong Kong/61.9/02 (H5N1) virus. These data suggest that the influenza A/chicken/Hubei/327/2004 (H5N1) virus which circulated in central China derived its internal gene from a virus similar to the influenza A/Chicken/Hong Kong/61.9/02 (H5N1) virus.

Amino Acid Sequence↗

PubMed2006

Development of a specific latex agglutination test based on a recombinant hemagglutinin protein to detect antibodies to H5 avian influenza viruses.

A latex agglutination test (LAT) was developed for rapid detection of antibodies to H5 avian influenza viruses (AIVs). The hemagglutinin protein of H5 AIV was covalently linked to carboxylated latex by ethyl-dimethyl-amino-propyl carbodiimide to prepare the sensitized latex beads. The LAT was evaluated with the hemagglutination inhibition (HI) assay as the reference test. The H5-LAT showed a sensitivity of 87.0% and specificity of 88.9% in detecting 126 serum samples from experimentally infected chickens and a sensitivity of 82.5% and specificity of 86% in detecting 587 field chicken serum samples from mostly vaccinated chickens. The agreement ratio between H5-LAT and HI was found to be 87.3% and 83.1% for the two groups of samples, respectively. Difficulty with background agglutination in stored chicken sera was overcome by serum pretreatment with either dried chicken liver powder or dilution buffer containing detergent Tween-20. The H5-LAT has advantages over a previously reported whole-virus LAT in terms of biosafety in preparation, chemical stability, and higher specificity. It is a rapid and simple test suitable for field monitoring of antibodies to H5-type AIV.

Animals↗

PubMed2003

[Changes of prostaglandin E2 levels in periapical exudates after root canal treatment].

OBJECTIVE: The aim of this study was to examine the levels of prostaglandin E2 in periapical exudates before and after root canal treatment. METHODS: Periapical exudates specimens were quantitatively sampled from root canals of 25 teeth at consecutive treatment visits, and the concentration of prostaglandin E2 in each sample was determined using a commercial kit. RESULTS: The mean prostaglandin E2 levels significantly decreased after the endodontic therapy. Significant correlations between the levels of prostaglandin E2 and the clinical features of periapical periodontitis were also found in this study. CONCLUSION: The results suggest that the level of prostaglandin E2 in periapical exudates reflects the state of periapical periodontitis.

Adolescent↗