Biomedical subjects
Publications and source records attributed to Hsien-Yeh Chou.
Conversion disorder is caused by previous severe stress, emotional conflict, or an associated psychiatric disorder, and usually presents with one or more neurologic symptoms. Clinically, it is challenging to diagnose diseases such as transient ischemia attack, stroke, brain tumor, spinal cord injury, and neuropathy. In this case report, we present a male stroke patient who had a typical conversion disorder.
It has been shown that mouse 24p3 protein is a dexamethasone-inducible secretory protein which suggests the involvement of an autocrine mechanism in the L929 cell line. The aim of this study was to investigate the possibility of this mechanism in L929 cells and to also demonstrate further processing of this protein after association with L929 cells. We have characterized the internalization of 24p3 protein in L929 cells with fluorescein isothiocyanate- and Alexa555-labeled protein supplement instead of secreted 24p3 protein. As endocytotic inhibitors could reveal the inhibition of protein internalization, we confirmed the existence of receptor-mediated 24p3 protein internalization in L929 cells by carrying out an inhibition test. Plus-chase experiment of L929 cells with biotinylated 24p3 protein demonstrated a release of internalized 24p3 protein into the extracellular region. The protein recycling inhibitor, bafilomycin A1, arrests the transport of 24p3 protein by accumulating in early endosome, but no effect could be observed in protein release from early to late endosome by nocodazole. These results provide the first evidence on recycling of apo-24p3 protein in L929 cells.