Biomedical subjects

Hua Yang

Publications and source records attributed to Hua Yang.

At least 91 records · Page 5Linked to original sources

PubMed2005

Detection of human immunodeficiency virus type 1 DNA in dried blood spots by a duplex real-time PCR assay.

A dried blood spot (DBS) is a well-accepted means for the collection, transport, and storage of blood samples for various epidemiologic, serologic, and molecular assays for human immunodeficiency virus (HIV) studies. It is particularly important for mother-to-infant-transmission studies of affected individuals living in remote areas. We have developed a real-time PCR method to detect HIV type 1 (HIV-1) DNA in dried blood spots. A cellular gene, RNase P, was coamplified with the HIV-1 DNA in the same tube to monitor the DNA extraction efficiency and the overall assay performance. Our assay is a one-tube, single-step closed-system assay and uses a dUTP/uracil DNA glycosidase anti-PCR contamination control. The HIV-1 primers and probe were derived from a conserved region of the long terminal repeat. The detection of RNase P is attenuated by lowering the forward and reverse primer concentrations so that its amplification will not overwhelm the HIV-1 amplification and yet will provide a semiquantitative measurement of the quality of the isolated DBS DNA. We examined 103 HIV-1-seropositive and 56 seronegative U.S. adults and found that our assay has a sensitivity of 98.1% (95% confidence interval [CI], 95.5% to 100%) and specificity of 100% (95% CI, 99% to 100%). The positive and negative predictive values are 100% and 96.6%, respectively. This duplex PCR assay may be useful in identifying HIV-1-infected persons, particularly infants born to seropositive mothers in remote areas of the world.

Adult↗

PubMed2005

Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase.

OBJECTIVE: To screen and evaluate the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 phosphatase. METHODS: The affinity chromatography purified glutashione-S-transferase/Cdc25A phosphatase fusion protein and Cdc2/cyclin B from the extracts of starfish M phase oocytes are used as the cell cycle-specific targets for screening the antimitotic constituents. We tested 9 extracts isolated from the Chinese medicinal herbs and vegetables including the agents currently used in cancer treatment by measuring the inhibition of Cdc25A phosphatase and Cdc2 kinase activity. The antitumor activity of the extracts was also evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry. RESULTS: Cdc25A inhibitory activity and antitumor activity are detected in the extracts isolated from three Chinese medicinal herbs Agrimona pilosa; Herba solani lyrati; Galla chinesis. CONCLUSION: We found three extracts isolated from Chinese medicinal herbs have potential inhibitory activity of Cdc25 phosphatase using a highly specific mechanism-based screen assay for antimitotic drug discovery.

Apoptosis↗

PubMed2005

[A citation analysis of National Journal of Andrology].

OBJECTIVE: To evaluate the academic level and the popularity of National Journal of Andrology. METHODS: According to the information of Chinese Medical Citation Index (CMCI), a statistical analysis was made of the amount and distribution of the originals published in National Journal of Andrology and cited by the journals included by CMCI. RESULTS: The originals published in National Journal of Andrology were highly qualified and influential, with their authors widely distributed all over China and even in some parts of the world. CONCLUSION: With its unique academic style and character, National Journal of Andrology is a main core periodical of medicine, as well as one of the most important information resources in the field of andrology in China.

Andrology↗

PubMed2005

[Drug concentration in axillary lymph nodes of patients with breast cancer after lymphatic chemotherapy].

BACKGROUND & OBJECTIVE: Lymph node status is one of the decisive prognostic factors of breast cancer. Chemotherapy targeting regional lymphatic tissues has emerged as a promising therapy for malignancies with high tendency to disseminate lymphatically. This study was to detect drug concentration in axillary lymph nodes of patients with breast cancer after lymphatic chemotherapy (LC), and to investigate effect of LC on accumulation of antitumor drugs in regional lymph nodes through comparing with the effect of intravenous chemotherapy (VC). METHODS: Sixty patients with breast cancer, confirmed by preoperative puncture biopsy, were randomized into 2 groups, 30 (LC group) were subcutaneously injected with 4 ml of carboplatin-activated carbon suspension (containing 20 mg of carboplatin) around the primary tumor, the other 30 (VC group) were intravenously injected with an equal dose of aqueous carboplatin. Every 6 patients from each group received modified radical mastectomy 1, 12, 24, 36, or 48 h after injection. Axillary lymph nodes were removed for pathologic examination. The concentration of carboplatin in nodes was detected by Zeeman atomic absorption spectrometry. RESULTS: A total of 275 axillary lymph nodes were resected, with 154 in LC group and 121 in VC group. Of the 275 lymph nodes, 136(49.5%) were from 23 patients (38.3%) had pathologically detected metastases. The concentrations of carboplatin were significantly higher in LC group than in VC group 1, 12, 24, 36, and 48 h after injection [(11.82+/-3.50) microg/g vs. (0.06+/-0.02) microg/g, (23.58+/-7.34) microg/g vs. (0.11+/-0.05) microg/g, (18.22+/-4.93) microg/g vs. (0.10+/-0.02) microg/g, (16.70+/-5.15) microg/g vs. (0.05+/-0.02) microg/g, and (14.62+/-4.29) microg/g vs. 0, respectively, P < 0.001]. Lymph node metastasis had no correlation with drug concentration (P > 0.05). CONCLUSION: Compared with VC, LC can effectively and continuously improve drug concentration in axillary lymph nodes of patients with breast cancer.

Adult↗

PubMed2005

[Study on association between gestational diabetes mellitus and sulfonylurea receptor-1 gene polymorphism].

OBJECTIVE: To investigate allelic frequency of sulfonylurea receptor-1 (SUR1) in gestational diabetes mellitus (GDM) women of Han nationality in north China in order to find out susceptible gene associated with GDM, and to assess the association between SUR1 allele and body mass index (BMI) and secretion of insulin. METHODS: Seventy cases of pregnant women were selected. It included 35 cases of pregnant women with GDM (GDM group), 35 cases of normal pregnant women (normal control group). Another 35 women patients with type 2 diabetes in the same period as T2DM group. By using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), we detected the distribution of SUR1 alleles frequency in all individuals of three groups. We also examined BMI, fasting insulin (INS(0)) and 2 h insulin levels (INS(120)). RESULTS: There was significantly increased c alleles frequency of SUR1 in GDM and T2DM women compared with normal pregnancy (70.0%, 71.4% and 52.9%, P < 0.05). Also significantly increased A alleles frequency of SUR1 in GDM and T2DM women was found compared with normal pregnancy (41.4%, 44.3% and 24.3%, P < 0.05). In GDM group, women carrying cc genotype had a higher BMI [(29.2 +/- 5.6) kg/m(2)], INS(0) [(14.9 +/- 8.7) mU/L] and INS(120) [(40.2 +/- 12.1) mU/L] as compared with those in carriers of other genotypes (ct and tt) (all P < 0.05). Also they had a lower insulin sensitivity (HOMA-IR) (3.9 +/- 2.5) as compared with carriers of ct genotype. Women carrying AA genotype had a higher INS(0) as compared with carriers of other genotypes (AG and GG) [(15.4 +/- 3.2), (12.1 +/- 4.5) and (11.5 +/- 4.8) mU/L, both P < 0.05]. Logistic regression analysis showed there was relationship between SUR1 cc genotype and severity of GDM (P = 0.005, RR = 25.128). CONCLUSIONS: There are -3t-->c and A/G polymorphism of SUR1 gene in the Han nationality. Alleles c of SUR1 are significantly implicated in the susceptibility to GDM. It suggests that the defect in SUR1 gene (cc and AA) may contribute to insulin hypersecretion, which might be the cause of increased body weight and decreased insulin sensitivity and genotype cc of SUR1 is connected with severe type of GDM. It might be the predictable index of GDM conversion to type 2 diabetes.

ATP-Binding Cassette Transporters↗

PubMed2005

[The value of frequency doubling perimetry in glaucoma screening of aged 40 or more population].

OBJECTIVE: To investigate the diagnosis value of the frequency doubling perimetry (FDP) screening program in the aged 40 or more natural population-based glaucoma screening. METHODS: In 3 natural villages of Daxing District and 4 northern metropolitan communities of Beijing, 4439 subjects aged 40 or over were screened for glaucoma from May to October in 2001. The screening protocol C-20-1 of the frequency doubling perimetry (FDP) was used in all subjects. For suspect glaucoma subjects, TOP threshold visual field testing with an Octopus perimeter, gonioscopy and stereo fundusgraphy were performed on different days. Detected Glaucoma patients were classified as mild (MD 5 dB or less), moderate (MD > 5 dB and < 10 dB) and advanced (MD 10 dB or more) degrees according to their Octopus test results. FDP parameters including test time, numbers of abnormal location, comtotalon score and score by quadrant were analyzed. Data from one eye of normal subjects and milder eye of optic nerve damage in glaucoma subjects were analyzed. RESULTS: After excluding subjects whose fixation error or false positive error was > 0.33, data from 4330 subjects (97.54%) were analyzed. If abnormality of one location or more was used as a FDP cutoff, and subjects were divided into normal and glaucoma two groups (excluded other eye diseases in non-glaucoma subjects); the FDP had a sensitivity of 50.70% (72/142), a specificity of 90.58% (2260/2340), an area under the ROC curve (AUC) of 0.74, a positive predictive value (PV) of 47.71%, a negative predictive value of 96.95%, a positive likelihood ratio (LR) of 14.83 and a negative LR of 0.51. If excluded the eyes without glaucomatous optic nerve changes and visual field damage, the FDP had a sensitivity of 76.39%, an AUC of 0.87, a positive PV of 40.74%, a negative PV of 99.25%, a positive LR of 22.34 and a negative LR of 0.24. The sensitivity of detecting mild, moderate and advanced glaucoma was 54.17%, 76.00% and 100.00%, respectively. There was strong negative correlation between FDP quadrant score and quadrant mean sensitivity (MS) of Octopus perimetry in glaucoma patients (Spearman correlation, Rs = -0.732, -0.628, -0.639, -0.679, all P = 0.000). CONCLUSIONS: The FDP screening protocol C-20-1 is a very rapid functional test for screening glaucoma in large natural populations aged 40 or more and is proven to be feasible. It has high specificity and good sensitivity for moderate and advanced glaucoma.

Adult↗

PubMed2005

[Ultrasonographic diagnosis of central slip rupture in the finger extensor mechanism of the proximal interphalangeal joint].

OBJECTIVE: To evaluate the accuracy of ultrasonographic diagnosis for central slip rupture of the finger extensor tendon mechanism of the proximal interphalangeal joint. METHODS: Sixty-seven consecutive patients with injuries to the proximal interphalangeal joint underwent ultrasonic examination with a linear 5-10 MHz hockey stick-shaped scanner head. Meanwhile three methods of physical examination were applied to assess the injury, and the results of ultrasonic detection and physical examinations were compared with surgical findings. RESULTS: Thirty-nine patients with central slip rupture of the finger extensor mechanism were confirmed. Ultrasonography achieved good consistence with surgical findings in injury detection. For ultrasound and physical examinations, the sensitivity for injury detection was 100% and 89.74% with specificity of 96.43% and 82.14%, respectively, showing significant difference (P<0.01). CONCLUSION: As an accurate noninvasive method, ultrasonographic diagnosis can identify central slip rupture in the extensor mechanism of the finger over the proximal interphalangeal joint in early stages.

Adolescent↗

PubMed2005

[Citation analysis of the Chinese Journal of Ophthalmology from 2001 to 2004].

OBJECTIVE: To study the aspects of citation of the Chinese Journal of Ophthalmology and the requirement and utilization of literatures by the scientists working in the field of ophthalmology. METHODS: The citation analysis was used to analyze the references cited in the 926 articles published in the Chinese Journal of Ophthalmology from 2001 to 2004. RESULTS: The citation rate was 91.79% and there were 8.05 citations per article. Citations from journals and books accounted for 91.97% and 7.85% of all citations, respectively. The Price Index was 35.50% and the self-citation rate was 8.40%. The citations from the high-ranked 17 journals accounted for 54.03% of all citations. CONCLUSIONS: The articles cited by papers published in the Chinese Journal of Ophthalmology cover a wide variety of journals. Most citations came from the English journals. The half-citing life is relatively longer and the Price Index is relatively low. It indicates that the authors' ability for using the recently published articles should be improved.

Bibliometrics↗

PubMed2005

[Analysis of the articles published in Chinese journal of ophthalmology during years 2001 to 2004].

OBJECTIVE: To study characteristics of the publication of Chinese Journal of Ophthalmology during years of 2001 to 2004. METHODS: Statistic method was used to retrieve the following parameters of Chinese Journal of Ophthalmology: numbers of articles, columns, citation frequencies, grants support, time lag from accepted to publication. RESULTS: The journal published 926 pieces of papers and 16 reports during years of 2001 to 2004. There were 24 columns; citation rates were 89.98%; papers published in the journal 19.53% got grant support. The times lag for publication was relatively longer. CONCLUSIONS: Chinese Journal of Ophthalmology is a high quality, specialized and core periodical in china.

Bibliometrics↗

PubMed2005

[A citation analysis of Chinese Journal of Ophthalmology].

OBJECTIVE: To evaluate the academic level and the popularity of Chinese Journal of Ophthalmology by an analysis of the citation of this journal. METHOD: According to the information of Chinese Medical citation Index (CMCI), we statistically analyzed the amount and distribution of the original papers in Chinese Journal of Ophthalmology cited by the journal included by CMCI. RESULTS: The average of original articles cited by other researchers is 4.17, many articles had a high citation rate. The authors of these articles were from 32 provinces and/or municipalities. Beijing, Guangdong and Shanghai were in the front of ophthalmic research. There were 354 citing journals, and self-citing rate was 8.87%. CONCLUSIONS: The Chinese Journal of Ophthalmology has published high quality articles, and has its own edition styles to keep its steady level of research. It is one of the most important information resources for the ophthalmology researchers and is one of the most important core medical journals in China.

Bibliometrics↗

PubMed2005

[Analysis of authors of articles published in Chinese Journal of Ophthalmology from 1995 to 2004].

OBJECTIVE: We aimed to analyze the authors, the organizations and regional distribution of these authors of Chinese Journal of Ophthalmology, and ascertain the core authors, core organizations and core regions. METHODS: Using quantitative analysis method, we analysed the authors, co-authors and the core authors of papers published between 1995 to 2004. Also, the distributions of districts and highly quantitative organization were determined statistically. RESULTS: There were 2143 articles published during the last ten years; 912 author had published only one paper, accounted for 70.59% of total first authors. There were 1853 articles with one or more co-authors, the cooperative rate and degree were 86.47% and 3.56, respectively. There were 290 papers with single author, which accounted for 13.53% of total papers. The number of papers from universities, institutes, affiliated hospitals and hospitals were 246 (11.48%), 176 (8.21%), 969 (45.22%) and 423 (19.74%), respectively. There were 82 core authors published 553 (25.81%) papers, while 20 highly-quantities organizations published 1016 (47.41%) papers. Core region was Beijing, which published 574 (26.78%) articles. CONCLUSIONS: Authors of the Chinese Journal of Ophthalmology have a wide distribution and highly cooperative rate. There are a group of active and talented core authors, who have a great influence on this journal.

Authorship↗

PubMed2005

[Citation analysis of Space Medicine & Medical Engineering].

OBJECTIVE: To evaluate the academic research level and the popularity of Space Medicine & Medical Engineering (SMME). METHOD: With the Chinese Medical Citation Index (CMCI), the amount and distribution of the originals of SMME, which were cited by the journal included in CMCI, were analyzed according to method of citation analysis. RESULT: The average of original papers cited by other researchers was 2.25, and this was more than other medical journals. The authors of these cited papers were from 13 Provinces in China, Beijing and Shannxi Province were in the front of this research area. There were 109 citing journals, and self-citing rate was 43.97%. CONCLUSION: A series of high quality papers has been published by the SMME, and its own edition characteristics keep its research level steady. Not only has the journal become an important information source of space medical engineering but also a core journal in medicine research as well.

Aerospace Medicine↗

PubMed2005

[Bibliometric analysis of Chinese Journal of Pediatrics].

OBJECTIVE: To evaluate the quality of articles and the popularity of Chinese Journal of Pediatrics. METHODS: With the database of Chinese Science & Technology Journal Citation Reports 2000-2003, the six indicators, including impact factor (IF), total cited numbers, immediate index, ratio of cited numbers by other journals to total cited numbers, number of citing journals, and cited half-life were evaluated and compared with other Chinese periodicals in pediatrics and related subjects. The cited numbers, distribution of cited areas, and cited institutions of published articles in Chinese Journal of Pediatrics 2000-2003 were analyzed. RESULTS: From 2000 to 2003, the impact factor of the Journal was 0.937, 1.025, 1.052, 1.252, total cited number was 1378, 1468, 1863 and 2396. Immediate index, citing journals, and cited half-life rose steadily, and the ratio of cited numbers by other journals to total cited numbers remained above 0.9. The average cited number of articles was 3.3. The cited authors were from 26 provinces or cities. CONCLUSION: The Chinese Journal of Pediatrics ranked at the top of Chinese pediatric journals with its highest IF and total cited numbers, which indicates that the journal has explicit editorial aims, stable continuity, and the increased influence in Chinese pediatric research. The stable growth of immediate index, number of citing journals and cited half-life show that the Journal has a high rate of reflection, the articles published in the Journal were paid more attention and have influenced researchers for longer periods and widely. The higher rate of citation by others and lower self-cited rate indicate that the Journal has been rigorous and realistic in editorial practice. The Journal is demonstrated to be one of the main medical core periodicals and one of the most important information sources in the field of pediatrics in China.

Bibliometrics↗

PubMed2005

[Citation analysis of the Chinese Journal of Preventive Medicine from 2001 to 2004].

OBJECTIVE: To study the information absorbency of Chinese Journal of Preventive Medicine and its half-citing life, to find out the core journals and characteristics of literature requested by scientists, and to analyze the trend of preventive medicine research. METHODS: The citation analysis was used to analyze the references cited in 766 articles in Chinese Journal of Preventive Medicine from 2001-2004. RESULTS: 505 (65.93%) of 766 articles have the 4 708 references, and the self-citation rate was 3.97%. The average number of references per paper was 6.15, and the maximum of references in one paper was 39. Main types of citations were from journals (87.43%) and the books (9.98%). The Price Index was 45.20%, and the self-citation rate was 3.97%. The percent of citations from the high-ranked 22 journals were 26.51%. CONCLUSIONS: The publications in Chinese Journal of Preventive Medicine have received more concerns than those from other major journals in medicine. Periodical and English literature were mostly used by the preventive researcher. Moreover, the articles published in the journal were able to keep closely along with the advances of the subject research.

Bibliometrics↗

PubMed2005

[The incidence of dementia among elderly people in Xi' an, China].

OBJECTIVE: To explore the incidence of dementia among elderly people in Xi'an and its related risk factors. METHODS: Subjects that had been studied on the prevalence of dementia were follow-up, and the incidence of dementia, Alzheimer disease (AD) , and vascular dementia (VD) were counted by person-years. The clinical diagnosis on dementia,AD and VD were based upon the 3rd Edition of Diagnostic and Statistical of Manual of Mental Disorder, Revised version (DSM-III-R) and NINCDS-ADRDA criteria. RESULTS: 2197 subjects of non-dementia being identified in 1998, were re-surveyed in 2001. Out of them, 47 new cases of dementia including 37 cases of AD and 8 cases of VD were identified, with an annual incidence rates of dementia, AD and VD as 0.68%, 0.54% and 0.12% among those of 55 years and over and 0.89%, 0.69% and 0.17% in 65 years and over, respectively. Analysis from single factor logistic regression showed that age and education but not gender were closely related to the occurrence of AD. On the contrary, age, hypertension and stroke were closely related to the occurrence of VD. CONCLUSION: The incidence of dementia in the "Xi' an cohort" was similar to that being reported from other countries. AD and VD were an age-related diseases but education seemed to have had great protective effect while lack of formal education served as risk factor to AD.

Age Distribution↗

PubMed2005

Targeting the phosphatidylinositol-3 kinase/Akt pathway for the treatment of cancer.

Phosphatidylinositol-3 kinase (PI3K)/Akt is overactivated in a wide range of tumor types, and this triggers a cascade of responses, from cell growth and proliferation to survival, motility, epithelial-mesenchymal transition and angiogenesis. Therefore, this pathway presents an exciting target for molecular therapeutics. In addition, ectopic expression of PI3K or Akt, especially constitutively activated PI3K (p110alpha) or Akt, is sufficient to induce the oncogenic transformation of cells and tumor formation in transgenic mice, as well as the development of chemoresistance. Inhibition of PI3K/Akt signaling induces apoptosis and inhibits the growth of tumor cells that have elevated Akt levels. The dependence of certain tumors on PI3K/Akt signaling for survival and growth has wide implications for cancer therapy, offering the potential for preferential tumor cell killing. In the past few years, a number of inhibitors of the Akt pathway have been identified by combinatorial chemistry, high-throughput and virtual screening, or traditional medicinal chemistry. This review focuses on ongoing translational efforts to therapeutically target the PI3K/Akt pathway.

Animals↗

PubMed2005

[Citation analysis of published articles in the Chinese Journal of Internal Medicine in the period of 2000-2004].

OBJECTIVE: To identify and analyze the scientific level and quality of the articles published in Chinese Journal of Internal Medicine. METHOD: Using the database of the Chinese Medical Citation Index (CMCI), the numbers and distribution of the cited original articles in Chinese Journal of Internal Medicine from 2000 to 2004 were analyzed according to the method of citation analysis. RESULT: The percentage of cited papers in all the published articles was 47.10% during the period, the average times of citation of an original article by other researchers is 4.30 and the top cited frequency was 133. There were 250 articles being cited 5 or more (25.43% of the total papers), and the times of citation from them were 2834 (67.01% of the total). 983 cited articles were written by 845 authors from different countries. The author of the most frequently cited articles wrote 6 papers and the authors with one cited paper were 751 (88.88% of all the authors). The cited authors were from 31 municipalities and provinces as well as from other countries. Beijing, Guangdong and Shanghai were in the front of the list of places with authors being frequently cited. Beijing was the top of the rank (44.74% of total authors). There were 266 institutes cited and the most frequently cited institute was Peking Union Medical College Hospital of Peking Union Medical College and Chinese Academy of Medical Sciences. There were 685 citing journals and the self-citing rate is 3.52%. CONCLUSIONS: The Chinese Journal of Internal Medicine has published high quality articles. As a core medical journal, it is the one of the most important information resources for the medical researchers.

Bibliometrics↗

PubMed2005

Bifidobacteria DNA induces murine macrophages activation in vitro.

Previous studies have shown that oligodeoxynucleotides containing unmethylated CpG motifs were used as adjuvants for immunoregulation and immune response. This study was to explore the activation effects of Bifidobacteria DNA containing unmethylated CpG motifs (CpG DNA) on murine macrophage J774A.1 cells. The genomic DNA of Bifidobacteria was extracted and purified, and the methylation degree of CpG motifs was tested. The phagocytic ability of the macrophages was detected by flow cytometry. The cytokines (IL-1beta, IL-6, IL-12p40 and TNF-alpha) levels in the culture supernatants of Bifidobacteria DNA treated J774A.1 cells were assayed by ELISA. The content of nitric oxide (NO) was detected by Griess reagent. After treated with Bifidobacteria DNA for 24 h, Nile Red stain increased in J774A.1 macrophage, which suggested that the lipid metabolism increased in the macrophages. The phagocytic ability and levels of NO and cytokines of IL-1beta, IL-6, IL-12p40 and TNF-alpha were significantly higher than PBS group and CT DNA group. The results indicated that Bifidobacteria DNA could activate murine macrophages J774A.1, which could provide scientific basis for the research and application of microorganism DNA preparation.

Animals↗