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Biomedical subjects

Huan Qi

Publications and source records attributed to Huan Qi.

7 recordsLinked to original sources

Pressure-flow studies in patients with benign prostatic hyperplasia: a study comparing suprapubic and transurethral methods.

AIM: To compare the use of the suprapubic puncture method versus the transurethral method in pressure-flow studies in patients with benign prostatic hyperplasia. METHODS: Twenty-three men with benign prostatic hyperplasia underwent both suprapubic and transurethral pressure-flow studies during a single session. Standard pressure-flow variables were recorded in all patients with both methods, enabling calculation of obstruction using commonly used grading systems, such as the urethral resistance algorithm, the Abrams-Griffith (AG) number and the Schaer linear nomogram. RESULTS: There were statistically significant differences between the methods in the mean values of maximum flow rate (P < 0.05), detrusor pressure at the maximum flow (P < 0.01), urethral resistance algorithm (P < 0.01), AG number (P < 0.01) and maximum cystic capacity (P < 0.01). Of the men in the study, 10 (43.5%) remained in the same Schaer class with both methods and 18 (78.3%) in the same AG number area. Using the transurethral method, 12 (52.2%) men increased their Schaer class by one and 1 (4.3%) by two. There were also differences between the suprapubic and transurethral methods using the AG number: 4 (17.4%) men moved from a classification of equivocal to obstructed and 1 (4.3%) from unobstructed to equivocal. CONCLUSION: The differences between the techniques for measuring intravesical pressure alter the grading of obstruction determined by several of the commonly used classifications. An 8 F transurethral catheter significantly increases the likelihood of a diagnosis of bladder outlet obstruction when compared with the suprapubic method.

Aged↗

[Relationship between programmed cell death 5 protein expression and prognosis of renal clear cell carcinoma].

OBJECTIVE: To study the role of programmed cell death 5 (PDCD5) protein in the oncogenesis and development of renal clear cell carcinoma and its association with the prognosis of the malignancy. METHODS: PDCD5 expression was assayed immunohistochemically in 46 cases of human renal clear cell carcinoma, and the patients' survival was followed up. RESULTS: PDCD5 staining in the adjacent normal tissue of the tumor was significantly stronger than that in the tumor tissue, and PDCD5 expression was significantly correlated with the tumor grade, stage and prognosis. The tumors of high grade with strong invasive ability had much less PDCD5 expression and lighter staining. The three- or five-year survival rates of patients positive for PDCD5 expression was much higher than that of patients negative for PDCD5 expression. CONCLUSION: PDCD5 is a potent inhibitor of malignant transformation of renal clear cell carcinoma and may serve as a major predictor for evaluating the malignant potential and prognosis of the tumor.

Adult↗

Wavelet-based estimation of EEG coherence during Chinese Stroop task.

Wavelet-based estimation of instantaneous EEG coherence was applied to investigate the synchronization of different brain regions whilst 10 subjects performing Stroop task presented in Chinese. In contrast to coherence based on Fourier transform, wavelet-based coherence, which does not depend on the stationarity of signals, applies an adaptive window to the frequency of the signal and has a more accurate time-frequency resolution. In the present study, a greater negativity for the incongruent situation than congruent situation appeared from 350 to 600 ms post-stimulus onset over frontal, central, and parietal regions, and significantly higher EEG coherences for the incongruent situation than congruent situation were observed over frontal, parietal, and frontoparietal regions from 100 to 400 ms at beta1 (13-18Hz) frequency band, which was found to be sensitive in the discrimination between congruent and incongruent situations. The findings in the present study may indicate that functional synchronization as indexed by EEG coherence at beta1 frequency band is enhanced at the earlier stage while processing the conflicting information from the incongruent stimulus, and that beta1 frequency band is close related to interactions of brain areas in the selected attention task.

Adult↗

[Nephron-sparing surgery for small renal cell carcinoma: clinical analysis of 21 cases].

OBJECTIVE: To evaluate the clinical effects of nephron-sparing surgery in patients with early-stage small renal cell carcinoma. METHODS: Nephron-sparing surgery was performed in 21 patients with renal cell carcinoma including 1 with solitary kidney, 3 with unilateral tumor and contralateral renal compromise, and 17 with unilateral tumor and normal contralateral kidney. The diameter of the tumors ranged from 1.5 to 6.0 cm, with a mean of 2.8 cm. The tumor diameter in 17 patients with normal contralateral kidney was less than 4 cm (mean 2.5 cm) and the average diameter in 4 patients with contralateral renal compromise was 4.2 cm. Sixteen cases were in stage T(1), 4 in stage T(2), and 1 in stage T(3). Of the 21 patients, 4 underwent tumor enucleation, 10 polar nephrectomy and 7 wedge resection. RESULTS: All patients were followed up for an average of 40.8 months (7 to 66 months). One patient suffered a right lung and mediastinum metastasis 3 years after the surgery later and 1 with chronic glomerulonephritis required dialysis 27 months after the operation. No surgical complication or local recurrence were found in other patients. CONCLUSION: As a safe and effective therapy for early-stage small renal cell carcinoma, nephron-sparing surgery can be considered as the gold-standard therapy for patients with lesions less than 4 cm in T(1) and T(2) stages of localized unilateral tumor with normal contralateral kidney.

Adult↗

[Association of S100A4 mRNA expression with differentiation and metastasis of renal cell carcinoma].

OBJECTIVE: To study the association of S100A4 mRNA expression level with the differentiation and metastasis of renal cell carcinoma (RCC). METHODS: Tissue specimens were obtained from 31 patients undergoing surgery for renal cell carcinomas between May 2001 and May 2004. Reverse transcriptional PCR was performed for amplification of S100A4 mRNA (212 bp) from 31 RCC tissues and matched normal kidney tissues besides the tumor, and the differential S100A4 mRNA expression was analyzed for its association with the clinical manifestations of the patients. RESULTS: S100A4 mRNA expression was detected in all the RCC tissues and normal tissues adjacent to the carcinoma using fluorescence method, and in 29 cases, S100A4 mRNA expression was significantly higher in the RCC tissues than in the matched adjacent tissues. One case had high expressions in both tissues. Patients with poorly differentiated RCC had significantly higher S100A4 expression index than those with well differentiated RCC (7.94 vs 5.06, P<0.001), and patients with metastasis also had higher expression index (9.61 vs 5.53, P<0.001). No obvious difference was noted in the expression index between granular cell tumor and clear cell carcinoma (6.98 vs 6.02, P>0.05). CONCLUSIONS: S100A4 may serve as a marker for assessing the progression of RCC and provide assistance for clinical therapeutic decisions. S100A4 mRNA expression is correlated to RCC differentiation and may facilitate prognostic evaluation of RCC. Differential expression of S100A4 mRNA is correlated to the metastasis of RCC.

Biomarkers, Tumor↗

[Expressions of hypoxia inducible factor-1alpha and vascular endothelial growth factor in human renal cell carcinoma].

OBJECTIVE: To explore the expression of hypoxia inducible factor-1alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF) in renal cell carcinoma (RCC) and their relationship. METHODS: The expression of HIF-1alphaand VEGF was examined in 42 patients with renal cell carcinoma by SABC methods of immunohistochemistry. RESULTS: The positivity rate of HIF-1alpha was 57.1% in the RCC tissues, and there was no expression in the tissue adjacent to the tumor and normal tissues. The positivity rate of VEGF was 61.9% in the tumor tissues, with significant difference from the control tissues (P<0.05). The expression levels of HIF-1alpha and VEGF were significantly higher in metastatic RCC than in non-metastatic RCC (P<0.01) tissues, and there was a significant correlation between HIF-1alpha and VEGF expressions (Kappa=0.41, P<0.01). CONCLUSIONS: HIF-1alpha is highly expressed in renal cell carcinoma tissues in close correlation with VEGF. HIF-1alpha and VEGF may serve as important indicators to evaluate the biological behaviors of RCC such as metastasis and prognosis. HIF-1alpha is an important regulator of VEGF.

Adult↗

[Selection of the imaging modalities for diagnosis of renal trauma: experience with 74 cases].

OBJECTIVE: To explore the diagnostic approaches and values of the imaging modalities for traumatic renal injuries. METHODS: The clinical records of 74 cases of renal trauma treated in Nanfang Hospital were retrospectively reviewed to assess the diagnostic value of intravenous urography (IVU), type B ultrasonography and computed tomography (CT). RESULTS: The positivity rates by IVU, type B ultrasonography, and CT were 89% (43/49), 80% (55/68) and 100% (51/51) respectively for the diagnosis of renal trauma. CONCLUSION: IVU is rapid and convenient, ultrasonography less costly and invasive, and CT accurate for diagnosis of renal traumas.

Adolescent↗