Biomedical subjects

Huan Zhao

Publications and source records attributed to Huan Zhao.

5 recordsLinked to original sources

PubMed2026

Protocol to identify genes required for cardiomyocyte development using Perturb-Seq.

While Perturb-Seq combines CRISPR-based screening with single-cell RNA sequencing (scRNA-seq), large-scale experiments are costly and its application during development is complicated by differentiation heterogeneity. Here, we present a protocol to identify genes required for cardiomyocyte development using Perturb-Seq. We describe steps for sgRNA (single guide RNA) library cloning and infection, cardiomyocyte differentiation, cell hashing, super loading, and scRNA-seq. We then detail procedures for sequencing, mapping, and data analysis. For complete details on the use and execution of this protocol, please refer to Sivakumar et al.1.

CRISPR↗

PubMed2006

Stress induced plant resistance and enzyme activity varying in cucumber.

When pathogens penetrate plant cells, some chemical secretions are elicited, and the mechanical signals in plant cell may be induced by the simultaneous physical pressure to change. Based on the previous cognitions, we investigated the plant resistance and the variation of anti-disease enzyme activity in cucumber leaves after mechanical stress loading. Results showed that the appropriate mechanical stimulation could significantly improve plant resistance and alter the activity of phenylalanine ammonial lyases (PAL) and POD, leading to synthesis of lignin. However, we found that the effects of the stress on these cellular fundamental events were eliminated when the adhesion between plasma membrane and cell wall was disrupted. We speculated that mechanical signal transduction in plants depend on the adhesion of plasma membrane-cell wall.

Cladosporium↗

PubMed2005

Stress stimulus induced resistance to Cladosporium cucumerinum in cucumber seeding.

Up to date, the studies of plant induced resistance have become the focus in plant pathology and physiology. During the course of pathogens penetrating the plant cell, besides of chemical secretion, the pathogens may generate mechanical signal caused by the physical pressure on the plant cell. In the non-host resistance, both the chemical signal and the mechanical stress signal are considered to have contribution to the entire defense reaction acted by the plant. The penetration of pathogen Cladosporium cucumerinum to cucumber is thought to be one of the model in research of plant induced resistance. In the current study, as a mechanical signal elicitor, the appropriate stress stimulus was proved to effectually induce the resistance of cucumber seedling to C. cucumerinum. After the treatment of the stress stimulus on leaves, the activities of resistance-related enzymes were significantly increased, such as phenylanine ammonia lyase (PAL), peroxidase (POD). Also, we found that stress stimulation may cause synthesis of lignin, which acts as the physical barrier to defense the pathogens. The results suggest that stress stimulation may not only enhance ability of the plant cell resistance to pathogen penetration but also elicit the accumulation of pathogens suppression or antimicrobial chemical substance in the plant cell.

Cladosporium↗

PubMed2005

Stress stimulation induced resistance of plant.

During the course of pathogens penetrating the plant cell, besides of chemical secretion, the pathogens may cause mechanical signal by the physical pressure on the plant cell. In the current study, we took the stress as the mechanical signal elicitor to find the effect of plant resistance induced by stress. The results showed that an appropriate stress stimulation can evidently improve the plant resistance. However, disruption the plasma membrane-cell wall adhesion will absolutely eliminate this kind of inducement effect, which suggests that the plant resistance inducement by stress depend on the adhesion of plasma membrane-cell wall. Also we found that stress stimulation may cause synthesis of lignin and increase the activity of phenylalaninc ammonial lyase (PAL) chitinase and beta-1,3-glucanase obviously. The results showed that stress stimulation may not only enhance ability of the plant cell resistant to pathogen penetration but also elicit the accumulation of pathogens suppression or antimicrobial chemical substance in the plant cell.

Cladosporium↗

PubMed2005

Effect of local stress induction on resistance-related enzymes in cucumber seeding.

In the current study, we found that the stress stimulus can act as a kind of elicitor, which can efficiently induce the resistance of cucumber against fungal pathogen. After the treatment of the stress stimulus on leaves, the activities of resistance-related enzymes were increased significantly. Such as phenylamine ammonia lyase (PAL), peroxidase (POD) and polyphenoloxidase (PPO), which are strongly associated with the plant disease resistance. Also the expression of pathogenesis-related protein (PR protein) were activated by stress stimulus, with the results that the activities of chitinase and beta-l,3-glucanase were increased obviously. The data showed that one of the mechanism of stress stimulus induction plant resistance may act via eliciting the metabolism related disease resistance within plant, which can produce many suppressing and antimicrobial compounds to against pathogens infection efficiently.

Catechol Oxidase↗