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Biomedical subjects

Hui Li

Publications and source records attributed to Hui Li.

At least 379 records · Page 21Linked to original sources
PubMed2002

Divergent effects of retinoic acids on the expression of ERalpha and 17beta-hydroxysteroid dehydrogenase type 2 in endometrial carcinoma cells (RL 95-2).

The effects of E2 are dependent on ERs and local E2 concentration in target cells. Modulation of intracellular E2 concentration involves the action of 17beta-hydroxysteroid dehydrogenase (17HSD) type 2, the enzyme converting E2 to estrone. In the present study, the influence of RAs on the growth of endometrial cancer cell line RL 95-2 as well as the expression of ERs and 17HSD type 2 have been investigated. It was found that RAs repress the growth of RL 95-2 cells, which express all subtypes of RXR and RAR, as examined by RT-PCR. Also, quantitative RT-PCR analysis showed that both ERalpha and ERbeta are present in RL 95-2 cells, and Western blot assay further revealed that ERalpha expression was decreased by all trans-RA treatment. In contrast, RAs induced 17HSD type 2 mRNA expression in a dose- and time-dependent fashion. This stimulatory effect was also detected at the level of in vivo oxidative 17HSD activity in cultured cells. On the other hand, the abundance of 17HSD type 2 mRNA was not altered by RAs in cultured normal epithelial cells isolated from human early- and late-secretory endometrium. The data indicate that RAs have an inhibitory effect on the growth of RL 95-2 cells and a cross-talk with the estrogen pathway in estrogen-responsive endometrial cancer cells.

17-Hydroxysteroid Dehydrogenases↗
PubMed2002

The sensitivity of digestive tract tumor cells to As2O3 is associated with the inherent cellular level of reactive oxygen species.

AIM: To explore the correlation of the inherent cellular ROS level with the susceptibility of the digestive tract tumor cells to apoptosis inducted by As2O3. METHODS: Two gastric carcinoma cell lines, SGC7901 and MKN45, and two esophageal carcinoma cell lines, EC/CUHK1(alternatively named EC1.71) and EC1867 with low concentration(2 micromol x L(-1))of As2O3 were cultured espectly, which confirmed the difference in apoptosis susceptibility between SGC7901 and MKN45, and between EC/CUHK1 and EC1867. The cells were incubated with dihydrogenrhodamine123 (DHR123), used as a ROS capture in absence of As2O3. The fluorescent intensity of rhodamine123, which was the product of cellular oxidation of DHR123, was detected by flow cytometry, and ROS was measured. RESULTS: Apoptosis induced by a low concentration of As2O3 was more readily to occur in SGC7901(22.4%+/-2.4%) and EC/CUHK1(27.0%+/-2.9%) than in MKN45(2.1%+/-0.5%) and EC1867(0.8%+/-0.5%). In other words, SGC7901 was more sensitive than MKN45 to As2O3, meanwhile EC/CUHK1 was more sensitive than EC1867 to As2O3. The level of inherent cellular ROS in SGC7901(650+/-37) was higher than that in MKN45(507+/-22)(P<0.01), and the level of inherent cellular ROS in EC/CUHK1(462+/-17) was higher than that in EC1867(187+/-12)(P<0.01). CONCLUSIONS: The cellular sensitivity to apoptosis induced by As2O3 is associated with the difference in cellular ROS level. The inherent ROS level might determinate the apoptotic sensitivity of tumor cells to As2O3.

Antineoplastic Agents↗
PubMed2002

[Cloning and sequencing of fatty acid binding proteins gene in chicken].

A pair of primers was designed according to the sequence of mammal fatty acid binding protein (FABP) gene, then PCR amplified to chicken genome. After the product of PCR was cloned and sequenced, homologous comparison was done among porcine heart fatty acid binding protein gene and porcine adipocyte fatty acid binding protein gene. The result showed that the sequence of chicken FABP gene had 68% and 75% homology with porcine H-FABP and A-FABP gene respectively, and had 75% homology with porcine AFABP on amino acid level. The result of Northern showed that the gene only expressed in fat tissues.

Adipose Tissue↗
PubMed2002

[Studies of single nucleotide polymorphism of PPAR gene and its associations with fattiness trait in chicken].

In this experiment, the AA broiler fat traits and three Chinese special breeds (Shiqiza, Beijing youji and Baier) were used to study the effect of PPAR-alpha gene on fat trait. Coding region of the gene was amplified by seven pairs of primers, and then single nucleotide polymorphisms (SNPs) were detected by the technique of single strand conformation polymorphism (SSCP) and finally confirmed by sequencing. One nucleotide variation was found, the result of chi 2 analysis showed that the distribution of three genotypes was very different among different breeds(P < 0.01). The result of variance analysis showed that the birds with BB genotype had a higher abdominal fat weight than the birds with other genotypes (AA and AB). It implied that PPAR-alpha gene could be a candidate locus or linked to a major gene to significantly affect abdominal fat traits in chicken.

Abdomen↗
PubMed2002

[Ascorbic acid enhances the apoptosis of U937 cells induced by arsenic trioxide in combination with DMNQ and its mechanism].

OBJECTIVE: To investigate whether ascorbic acid could enhance the efficacy of arsenic trioxide (As(2)O(3)) combined with 2, 3-dimethoxy-1, 4-naphthoquinone (DMNQ) in inducing the apoptosis of leukemia cell line U937 and its possible mechanism. METHODS: Flow cytometry and electron microscopy were applied to detect apoptosis of U937 cells after treatment with various combinations of As(2)O(3), DMNQ and ascorbic acid for 24 hours. RESULTS: As(2)O(3) and DMNQ induced-apoptosis of U937 cells was enhanced (35.24%-->61.20%) upon cotreatment with ascorbic acid. Catalase could reverse this effect of DMNQ. Ascorbic acid had no effect on DMNQ-induced apoptosis of U937 cells. CONCLUSION: Ascorbic acid enhanced the apoptosis of U937 cells via reactive oxygen species-dependent pathway in the presence of As(2)O(3).

Apoptosis↗
PubMed2002

[A case-control study on family history of Alzheimer's disease].

OBJECTIVE: To explore the relationship between Alzheimer's disease and its family history of the patients. METHODS: Stratified analyses and logistic regression analysis were used to examine the association between Alzheimer's disease and its family history exposure in 127 cases and 254 matched controls from a population-based case-control study. RESULTS: The risk of Alzheimer's disease was significantly higher in those who had at least one first-degree relative with dementia or major psychosis as compared to those who had no dementia or major relatives with psychosis (OR = 6.25; 8.33). Adjusted for age and level of education, family history of dementia was still associated with Alzheimer's disease positively (OR = 2.07). CONCLUSION: This study provides evidence that familial aggregation of Alzheimer's disease might exist among people living Beijing.

Adult↗
PubMed2002

[The study of the uncoulping protein gene as the candidate gene for fatness traits in chicken].

The UCPs are integral membrane proteins of the mitochondrial respiration from oxidative phosphorylation, diminishing the resulting production of ATP and instead yielding dissipative heat. The action of those proteins creates a futile cycle that decreases the metabolic efficiency of the organism. Thus UCPs provide new clue to obesity's causes. This study was designed to investigate the effect of UCP gene on chicken fatness traits. The fifth generation population of divergent selection broiler line, Hyline Brown layer and three native breeds (shiqiza, Beijing You, baier) were used in this research. Body weight and body composition traits were measured in broiler lines at 7 weeks of age. Primers for the 3'-untranslator region in UCP were designed from database of chicken genomic sequence. Polymorphisms were detected by PCR-SSCP and DNA sequencing. The results showed that there was significant difference (P < 0.01) in the frequency of genotype among breeds except broiler vs Beijing You and Baier vs Hyline Brown layer in mutation sites detected by the two pairs of primers. The distribution of genotype in Beijing You and broiler had no difference. It deduced that Beijing You belongs to the native breed that has dominant meat type traits and has the same genetic background with broiler. Baier and Hyline Brown Layer have no difference in the genotype, it can be viewed as they have same genetic background. A A/C mutation at base position 1197 was found among individuals in broiler line and the least square analysis showed that BB birds had significant lower (P < 0.01) abdominal fat weight and percentage of abdominal fat than AB or AA birds. From the results we can putatively draw the conclusion that UCP gene is the major gene to affect the fatness traits or it links with the major gene.

Animals↗
PubMed2002

Apoptosis susceptibility of tumor cells to arsenic trioxide and the inherent cellular level of reactive oxygen species.

OBJECTIVE: To explore the association of inherent cellular reactive oxygen species (ROS) levels with susceptibility of the tumor cells to apoptosis induction by arsenic trioxide (As(2)O(3)). METHODS: Low concentration (2 micromol/L) of As(2)O(3) was administered to two cultured leukemic cell lines, NB4 and U937, and two esophageal carcinoma cell lines, EC1.71 (also named EC/CUHK1) and EC1867, to confirm the difference in apoptosis susceptibility of NB4 versus U937 and of EC1.71 versus EC1867. Dihydrogenrhodamine 123 (DHR123), used as a ROS capture agent, was incubated with cells in the absence of As(2)O(3). Fluorescence intensity of rhodamine 123, the product of cellular oxidation of DHR123, was detected by flow cytometry and ROS was measured. RESULTS: Low concentration of As(2)O(3) induced apoptosis was more likely to occur in NB4 and EC1.71 cells than in U937 and EC1867 cells, or NB4 was more sensitive than U937, and EC1.71 more sensitive than EC1867 to As(2)O(3). The inherent cellular ROS level is higher in NB4 than in U937, and also higher in EC1.71 than in EC1867. CONCLUSIONS: The difference in cellular ROS level is positively associated with cellular susceptibility to apoptosis induction by As(2)O(3). The inherent ROS level might be important in defining apoptotic susceptibility to As(2)O(3).

Antineoplastic Agents↗
PubMed2002

[Single nucleotide polymorphism analysis of the chicken Myostatin gene in different chicken lines].

Myostatin is a new member of the TGF-beta superfamily. It is specifically expressed in skeletal muscle cells and functions as a negative regulator of skeletal muscle growth. This study was aimed to identify the single nucleotide polymorphisms (SNPs) of Myostatin gene in various breeds including Beijing Youji, Baierji, Shiqiza, Dwarf Yellow Chickens, Mini Yellow Chickens, Huiyang Huxuji, Recessive White Chickens, AA and Hyline Layer. The 17 pairs of primers for Myostatin gene were designed based on the chicken genomic sequence. The total 5 SNPs were identified in the chicken Myostatin gene by PCR-SSCP of which 3 were in the 5'-regulatory region and 2 were in the 3'-regulatory region of the Myostatin gene in different chicken lines. The sequence data showed that one of the three SNPs localized in the 5'-regulatory region was due to 3 single point mutations (G304A, A322G, C334T, respectively), other two were single point mutation of G167A and T177C, respectively. The other two SNPs in the 3'-regulatory region of the Myostatin gene are A to T (7263) and A to G (6935). Population genetic analysis indicated that genotype frequencies of the Myostatin locus (P60/P61) in Beijing youji were quite different from those of other tested chicken lines. Frequency of the BB genotype was very high (0.70) while the AA genotype was only 0.033 in 5'-regulatory region (P60/P61) in Beijing youji population. The genotype frequencies (P93/P94) were significantly different among the lines (P < 0.01): frequencies of the EE genotype in Beijing Youji were lower than those in the other lines, while frequencies of the EE genotype in Baierji and Hyline Layer were higher than the rest. Frequency of allele C (P80/P81) in 9 breeds was higher than that of allele D. Frequency of genotype MM (P76/P77) was low, and that of MN was high in 7 breeds.

Animals↗
PubMed2002

Effect of seawater immersion on plasma osmotic pressure and electrolyte balance following open chest trauma.

OBJECTIVE: To explore the effect of seawater immersion on serum osmotic pressure and electrolytes balance following chest trauma in dogs. METHODS: Twenty-five healthy adult dogs were used in the experiment. A canine model of right open pneumothorax was established by chest puncturing on all animals. Animals were divided into three groups: a control group (n=10) with chest trauma without any immersion; a seawater group (n=10) immersed in seawater after chest trauma and a normal saline group (n=5) immersed in normal saline solution following chest trauma. Blood samples were taken at different time intervals to determine plasma osmotic pressure and electrolytes. The hemodynamic changes were also recorded. RESULTS: Mortality in the seawater group was much higher than that of the control group and the normal saline group. The mean survival time in the seawater group lasted only 45 minutes, while in the control group and the normal saline group the average survival time was more than 4 hours (P<0.01). One of the most important causes of death was hypernatremia and high osmolality. Severe electrolytes imbalance was observed in seawater group. Hypernatremia and high osmolality were the most significant factors of high mortality in the seawater group. CONCLUSIONS: Seawater immersion after chest trauma appears to be associated with severe electrolyte imbalance as well as high osmotic pressure. These may be the risk factors leading to fatal outcome.

Animals↗
PubMed2002

[The analysis of the surnames and intermarriages of the suburbanites of Shanghai].

The origins of the suburban native of shanghai are varied. With the methods of correlation analysis and principal analysis, we random sampled the surnames of 13 villages and towns located in the districts of Shanghai, and found that Jading, Pudong, Nanhui and the east part of Fengxian make up the group of northeast part, while Jinshan, Songjiang, Qingpu and Chongming make up a completely different one from the other. The surnames of Nanxiang's origin are complex, while Maqiao and Jinhui's are of special kind. The surnames recorded in pedigree have apparent correlation with the modern ones. Moreover, the surnames of northeast part are mainly derived from the areas north to Yangtze River, but those of southwest part come from the areas south to Yangtze River. This explains that the origins of the two districts' throng may be different. The residents of the northeast part, which formed land comparatively recent, probably came from north areas after Tang and Song periods, while those of southeast part came from other northern areas. The residents of shell bar districts such as Maqiao and Jinhui have inherited relation with the culture of remote antiquities of Maqia. The contributing factors of surname are diversified. By researching into the scope of intermarriage of 20th century in Jinhui, we found that before 70's, the intermarriage radius was fixed in 5 kilometers because of match maker custom; in the 70's & 80's, unrestrained love reduced the intermarriage radius into same village about 3 kilometers; after in 90's, the intermarry radius expand to every province because of the rapid social and economic development. The intermarriage scope and consanguinity natural diffuseness can stand for the situations in shanghai and in large parts of south China. The assimilation of adjacent villages under matchmaker custom takes over 2000 years, while takes over 7000 years under unrestrained love. We can see that the impact of the natural diffuseness is rather weak, and the mixed living of emigrants is the main factor. The history of every colony's adjacent living is far shorter than that of assimilation. So there is almost no mixed race in this period. The most parts of south China's situations should be similar. The broken of traditional intermarry suggests us that the auto-disappear speed of abundant genetic resource is high. It is now the most pressure time to gather and research our genetic resource.

Adolescent↗
PubMed2002

[Prevention of gastroesophageal reflux and aspiration in neonates by holding with hands immediately after delivery by cesarean section].

OBJECTIVE: To evaluate the clinical effect of preventing gastroesophageal reflux (GER) and aspiration in neonates delivered by elective cesarean section with hand immediately after birth. METHODS: On the basis of the principles for drowning resuscitation and evaluation of the principal factors causing GER and aspiration, the author adopted immediate manual management to promote emptying of gastroesophageal and airway contents in 496 newborns delivered by elective cesarean section. RESULTS: No GER and aspiration occurred in the babies receiving the management, and no complication was recorded. CONCLUSIONS: As an early preventive measure, manual management is effective in clearing gastroesophageal and airway contents against GER and aspiration, and Apgar scoring can be readily conducted.

Cesarean Section↗
PubMed2002

The relationship between nonresponse to hepatitis B vaccine and HLA genotype/haplotype.

OBJECTIVE: To study the relationship between the nonresponse to hepatitis B vaccine and HLA genotype/heplotype in Chinese population and provide the evidence for explaining the genetic mechanism of this nonresponse. METHODS: Our research focused on the relationship between nonresponse to Hepatitis B vaccine and HLA-DRB1, DRB3, DRB4, DRB5 and DQB1 genotype/haplotype in Chinese population, collected from a community in Guangxi Zhuang Autonomous Region. The group specific amplification was employed to characterize 107 individuals' genotype and haplotype of HLA clusters. Different models statistics such as relative risk test, correlation test and linkage disequilibrium analysis were used to analyze the data. RESULTS: The results showed that there is a linkage disequilibrium between nonresponse to Hepatitis B vaccine and HLA haplotype DR4, 1122 (DRB1 * 0401- 22, 1122)-DR53 (DRB4 * 0101101, 0102/3)-DQB4 (DQB1 * 04). CONCLUSION: In Chinese population, nonresponse to hepatitis B vaccine is highly associated with special HLA haplotye.

Asian People↗
PubMed2002

[Study on the near, middle and long distance stereopsis of normal and children with intermittent exotropia].

OBJECTIVE: To study the pre- and post-operative changes of stereopsis at different distances in normal and children with divergence in excess type of intermittent exotropia. METHODS: TNO and new stereo tests (NST) and the middle and long distance stereotest were used for the evaluation of stereoacuity at 40 cm - 5 m in 844 normal and 35 children aged 4 - 12 years with divergence excess type of intermittent exotropia undergoing operative correction of strabismus. RESULT: (1) Normal subjects: The TNO test showed that the near distance stereoacuity </= 60" was in 98% subjects aged 4 - 8 years. The stereoacuity was increased with the increase of age, the correlation being linear. The stereoacuity in children aged 10 - 11 years was the lowest. In the comparison of stereoacuity between children aged 4 - 8 years and 9 - 12 years, the difference in distribution was very significant (P < 0.01). The NST test showed that the near stereoacuity >/= 40" was in 97% subjects. The mean stereoacuity was 40" - 42" in children various in age. The middle and far distance stereoacuity was the greatest in children aged 4 years, along with the increase of age, it was gradually declined and at 9 - 10 years it was the lowest. (2) Divergence excess type of intermittent extropia: There was no significant difference between the pre- and post-operative near stereoacuity examined by TNO and NST (P > 0.05) The pre-operative middle and long distance stereoacuities were greater than that in the normal children, the difference being significant (P < 0.05). The post-operative middle distance stereoacuity was improved and approximately normal in comparison with that before the surgery, and the far distance stereoacuity was improved mildly. CONCLUSIONS: (1) In the normal group, the development of stereopsis at different distances begins at 4 years, keeping on developing with the increase of age, and the stereoacuity is elevated after 8 years and in 9 - 11 years well developed. (2) In the group of divergence excess type of intermittent exotropia, the pre-operative near stereoacuity is well, but the middle and far distance stereoacuities are poor and the middle stereoacuity improves significantly after the surgery. The recovery of middle distance stereoacuity seems to be an objective criterion of the operative therapeutic effect.

Age Factors↗
PubMed2002

[SSSR fingerprinting in broiler selected for very low density lipoprotein (VLDL)].

SSR fingerprints were analyzed in three generations of fat line (FL) and lean line (LL) of broiler chickens. Changes in gene frequencies of every locus were evaluated. Thus the relationship between SSR markers and VLDL (a trait representing fat mass of broiler), which is the basis for early selection of LL broiler, was examined. Fourteen microsatellite locus were successfully amplified with 4 of 5 primers used. The results of chi2 test for the gene frequencies of every locus show that one locus was significantly different in generation 1(P<0.05), two in generation 2 and 4 in generation 3.

English Abstract↗
PubMed2002

[Single nucleotide polymorphism analysis in chicken leptin receptor exon 9].

Leptin receptor is a type I cytokine super family member and plays an important role in leptin functioning signal transduction. This study was designed to investigate the single nucleotide polymorphism (SNP) of OBR gene in various breeds, including Fatness Line (FL), Leanness Line (LL), Beijing Youji, Baierji, Shiqiza, Dwarf Yellow Chickens, Mini Yellow Chickens, Huiyang Huxuji, Recessive White Chickens and Hyline Layer. The primers for exon 9 in OBR gene were designed from the database of chicken genomic sequence and the SNPs were detected by PCR-SSCP method. One SNP (C/A at 1167 in cds) was found among individuals within all breeds. However, the amino acid was not changed because it was a silence mutation. The result of population genetics analyses showed that the frequency of AA genotype in Beijing Youji was significantly higher than that in other lines. Also, the frequency of A allele in FL was significantly higher than that in LL.

English Abstract↗
PubMed2001

The Susceptibility of Leukemia Cells to Arsenic Trioxide-induced Apoptosis is Determined by Cellular Reactive Oxygen Species Level.

To explore the relationship between the susceptibility to arsenic trioxide (As(2)O(3))-induced apoptosis of leukemia cells and the level of reactive oxygen species (ROS) of cells, flow cytometry and electron microscopy were applied to identify apoptosis, and dihydrorhodamine123 was used to display the ROS level of cells. As(2)O(3) alone or in combination with 2,3-dimethoxy-1,4-naphthoquinone (DMNQ, 2.5 &mgr;mol/L for NB4 cells, 10 &mgr;mol/L for U937 cells) were used to induce cell apoptosis. The results showed that NB4 cells possessed higher level of ROS than U937 cells. DMNQ raised ROS levels of NB4 and U937 cells, sensitized U937 cells to As(2)O(3)-induced apoptosis, and enhanced the efficacy of As(2)O(3)-induced apoptosis of NB4 cells. Catalase reversed the effect of DMNQ on NB4 and U937 cells. It was concluded that the susceptibility of leukemia cells to arsenic trioxide-induced apoptosis is determined by ROS level in the cells.

Journal Article↗