Biomedical subjects

Hyeong-Cheol Yang

Publications and source records attributed to Hyeong-Cheol Yang.

21 records · Page 2Linked to original sources

PubMed2003

Actin comet tails, endosomes and endosymbionts.

The Arp2/3 complex consists of seven highly conserved and tightly associated subunits, two of which are the actin-related proteins Arp2 and Arp3. One of the best-studied functions of the Arp2/3 complex is to stimulate actin nucleation and force production at the leading edge of motile cells. What is now clear is that Arp2/3-complex-mediated force production drives many intracellular movements, including movement of bacterial pathogens in infected host cells, internalization of extracellular materials via phagocytosis and endocytosis, and movement of mitochondria during cell division in budding yeast. Here, we describe recent advances in the mechanisms underlying Arp2/3 complex-driven intracellular movement.

Actins↗

PubMed2002

Actin cable dynamics in budding yeast.

Actin cables, bundles of actin filaments that align along the long axis of budding yeast, are crucial for establishment of cell polarity. We fused green fluorescent protein (GFP) to actin binding protein 140 (Abp140p) and visualized actin cable dynamics in living yeast. We detected two populations of actin cables: (i) bud-associated cables, which extend from the bud along the mother-bud axis, and (ii) randomly oriented cables, which are relatively short. Time-lapse imaging of Abp140p-GFP revealed an apparent increase in the length of bud-associated actin cables. Analysis of movement of Abp140p-GFP fiduciary marks on bud-associated cables and fluorescence loss in photobleaching experiments revealed that this apparent elongation occurs by assembly of new material at the end of the cable within the bud and movement of the opposite end of the cable toward the tip of the mother cell distal to the bud. The rate of extension of the tip of an elongating actin cable is 0.29 +/- 0.08 microm/s. Latrunculin A (Lat-A) treatment completely blocked this process. We also observed movement of randomly oriented cables around the cortex of cells at a rate of 0.59 +/- 0.14 microm/s. Mild treatment with Lat-A did not affect the velocity of movement of randomly oriented cables. However, Lat-A treatment did increase the number of randomly oriented, motile cables per cell. Our observations suggest that establishment of bud-associated actin cables during the cell cycle is accomplished not by realignment of existing cables but by assembly of new cables within the bud or bud neck, followed by elongation.

Actins↗

PubMedDate not supplied

Color and translucency of A2 shade resin composites after curing, polishing and thermocycling.

This study determined the differences in values and changes of color and translucency of eight brands of A2 shade resin composites after curing, polishing and thermocycling (TC). The color of specimens 10-mm in diameter and 2-mm thick was measured on a reflection spectrophotometer with SCE geometry under D65 illumination over a white and black background. Measurements were obtained before curing, after curing, after polishing and after TC. The color change (deltaE*ab), translucency parameter (TP) and contrast ratio (CR) were then compared. The range of deltaE*ab after curing was 3.8 to 10.2 (average deltaE*ab for the eight composites = 6.4), which was deemed perceptible to the observer. Polishing caused deltaE*ab of 1.9 to 4.5, which was perceptible in five of the eight composites. After 2,000 TC, deltaE*ab was 0.4 to 1.3. TP values tended to increase after curing and decrease after TC (range before curing was 7.1 to 17.2). Changes in TP values after curing were statistically significant in all composites (p<0.05). Changes in CR values were similar to the translucency changes in TP. Though the composite shades were all designated as A2, color coordinates, TP and CR values, changes in color and translucency after curing, polishing and thermocycling varied by brand.

Color↗