Biomedical subjects
Publications and source records attributed to Hyesun Paik.
Organic artificial proteases with broad substrate specificity were synthesized by covering the surface of silica gel with aldehyde and the functional groups present in amino acids. The artificial proteases hydrolyzed ovalbumin, albumin, hemoglobin, gamma-globulin with half-lives as short as 50 min at 25 degrees C or 7 min at 50 degrees C.
Aspartic protease analogues synthesized by covering the surface of silica gel with carboxyl groups effectively hydrolyzed hemoglobin and gamma-globulin. It is proposed that the carboxyl group is involved in both complexation of the protein substrate and the catalytic cleavage of the peptide bonds of the complexed proteins.
An artificial protease synthesized by covering the surface of silica gel with aldehyde and indole groups effectively hydrolyzed albumin and gamma-globulin. It is proposed that the aldehyde group is involved in both complexation of the protein substrate and the catalytic cleavage of the peptide bonds of the complexed proteins.