[A new immunosorbent for purifying interferon].
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Biomedical subjects
I A Baschenko
Publications and source records attributed to I A Baschenko.
[Biological properties of immunochemically pure tetanus antitoxin].
Immunochemically pure tetanus antitoxin obtained from enzyme-treated horse serum is less reactogenic and anaphylactogenic and possesses higher therapeutic properties than antitoxin purified by nonspecific physico-chemical methods and containing ballast antigens. Due to its increased persistence in the recipient's body, the immunochemically pure antitoxin induces passive immunity in considerably lower doses than the preparations purified by the method "Diaferm-3".
Anaphylaxis↗
[High performance liquid chromatography identification of chloracetophenone in ocular tissues in burns induced by gas balloons (an experimental study)].
The kinetics of chloracetophenone (CN) in rabbit eyes was studied by high-performance liquid chromatography on a model of experimental third-degree burn. By the end of the first hour after exposure to the lacrimate, CN content was high in all the studied tissues: 28.5 +/- 0.563 x 10(-1) mg/kg in the cornea, 5.08 +/- 0.193 x 10(-1) mg/kg in the anterior chamber humor, and 3.26 +/- 0.123 x 10(-1) mg/kg in the vitreous. After 6 h the content of the irritant dropped almost threefold and was 8.5 +/- 0.403 x 10(-1) mg/kg in the cornea, 1.23 +/- 0.062 x 10(-1) mg/kg in the anterior chamber humor, and 0.017 +/- 0.006 x 10(-1) mg/kg in the vitreous. By the end of 24 h these values were 6.6 +/- 0.221, 1.46 +/- 0.123, and 0.015 +/- 0.005 x 10(-1) mg/kg, respectively, and by day 14 only trace amounts of CN were detected. Hence, CN in the Cheremukha gas balloons can penetrate into the inner structures of the eye and cause severe injuries. Persistence of CN for up to 14 days disordered the metabolic processes and anatomy of the eye. High content of CN during the first 7 days after burn requires adequate pathogenetic therapy.
Animals↗
[The use of highly purified staphylococcal alpha-toxin in the immunochemical purification of antibodies].
A simple procedure is described for isolation of highly purified staphylococcal alpha-toxin (SAT) by means of sorption on macroporous glass beads and gel filtration. Various conditions were tested of affinity immunosorption of antibodies and SAT. Highly-porous polyacrylamide gel activated by glutaraldehyde was found as a readily available and selective immunosorbent. The immobilized antigen was stabilized using reduction of azomethyne bonds. Specific activity of antibodies was elevated as the SAT purification degree increased. Correlation between antihemolytic and antilethal activities was unaltered during the immunochemical purification of antibodies.
Animals↗
[An immunosorbent for interferon purification].
Immunoaffinity chromatography was shown to be the method achieving the most complete elimination of antigenic admixtures from leukocyte interferon preparations without the loss of the preparation activity. An affinity sorbent has been developed on the basis of covalently linked polyvinyl alcohol (PVA). The immobilization of the antigen-specific rabbit globulin in the preparation of the sorbent is achieved by reaction of protein amino groups with the activated matrix. The proposed sorbent achieved the elimination of the antigenic admixtures from interferon preparations as effectively as those prepared on the basis of sepharose 4B, the productivity of the purification process being at least 5 times higher. The proposed sorbent is stable at the limit values of rH, is not destroyed by detergents, is sterilized in the process of preparation. Owing to the strong linkage of the immobilized immunoglobulin with the PVA-carrier, immunoaffinity chromatography on this sorbent does not involve contamination of the preparations with rabbit globulin allergenic for man. The combination of a large pore structure, wetting ability, stiffness, mechanical and chemical stability allows the proposed sorbent to be recommended for use in modern large-scale biotechnological production.
Animals↗