Biomedical subjects
Publications and source records attributed to I A Conti-Díaz.
The first case in Uruguay of 'tinea nigra' is described in a 44-year-old male patient with a maculous pigmented lesion on the right foot. It represents the most meridional case of the disease yet recorded in South America. Exophiala werneckii was isolated in cultures (strain 1905 IHM).
Sporothrix schenckii antigens were tested with seventy-nine sera from human cases of sporotrichosis for their value in the serological diagnosis of extracutaneous forms of the disease. 55.7% of the sera reacted in the tube agglutination test; 29.1% of them were positive by counter immunoelectrophoresis and only 7.6% reacted in the simple immunodiffusion test. There was a clear correlation between agglutination titers and the respective reactivity of sera by counter immunoelectrophoresis. By immunoelectrophoresis, 2 different anodic precipitin bands were observed between S. schenckii metabolic antigen and anti-Sporothrix hyperimmune animal sera. Since no false positive reactions were found in sera from patients with other mycoses, and considering the simplicity of the antigen preparation, we agree with previous authors that these methods may be useful for the detection of visceral forms of sporotrichosis.
Using the technique of immunoelectroosmophoresis-immunodiffusion (IEOP-ID), two antigenic fractions of Paracoccidioides brasiliensis one of them species-specific, produced precipitin bands in both the cathodic and anodic zones. Reactions of complete identity among the bands formed in these zones were demonstrated by a modification of the technique, employing additional wells around the central antigen well. Such bands would correspond to a simple diffusion of the corresponding antigenic fractions rather than to active electrophoretic migration.
Three agar immunoprecipitin techniques (double immunodiffusion, immunoelectrophoresis and immunoelectroosmophoresis-immunodiffusion) made with paracoccidioidin and serum of a paracoccidioidomycosis patient's wife, permitted us to observe a specific band in 6 successive samples of blood taken from the wife over a period of 21 months. The case represents an example of a subclinical paracoccidioidomycosis infection that is usually diagnosed in its disseminated, progressive form. Three possible sources of infection are discussed. But an interhuman contagion appears to be the most feasible.
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Five cases of tinea corporis in children by Microsporum gypseum are presented. In four of them an endothrix type of parasitism with arthrospores 4 to 8 microns in diameter was observed. In the other case ectothrix and endothrix types of parasitism were seen in different hairs. Endothrix infection of hairs by species of the genus Microsporum has not been clearly demonstrated until the present. Three isolates were identified as Nannazzia gypsea of the "+" mating type.
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