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Biomedical subjects

I A Harper

Publications and source records attributed to I A Harper.

11 recordsLinked to original sources

Diagnosis of neonatal chlamydial conjunctivitis.

Fifty seven babies with ophthalmia neonatorum had conjunctival smears examined by microscopy and bacterial culture, and by immunofluorescence, to find out which was the best method of diagnosing chlamydial conjunctivitis. The positive (33%) and negative (70%) predictive values of microscopy and culture were too low for us to accept it as an adequate method of detecting the presence of Chlamydia trachomatis.

Bacteriological Techniques

Isolation of chlamydia in irradiated and non-irradiated McCoy cells.

Specimens from eye and genital tract were cultured in parallel in irradiated and non-irradiated McCoy cells and the frequency of isolation of chlamydia using these culture methods was compared. There was a significant difference between the frequencies of isolation; irradiated McCoy cells produced a greater number of positive results.

Bacteriological Techniques