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Biomedical subjects

I A Kim

Publications and source records attributed to I A Kim.

At least 19 recordsLinked to original sources
PubMed2001

Potential of adenoviral p53 gene therapy and irradiation for the treatment of malignant gliomas.

We investigated the combined effects of p53 gene transfer and irradiation and its still unclear interaction mechanism in human gliomas. Four human glioma cell lines expressing mutant type p53 (U373 and A172) and wild-type p53 (D54MG and EFC-2) were transfected by adenoviral vectors bearing p53 gene at 50 multiplicity of infection. Two days after transfection, cells were irradiated (3, 6, and 9 Gy). The cytotoxicity was evaluated by clonogenic assay. The quantitative analysis of apoptosis and cell cycle analysis were performed using flow cytometry. Irradiation combined with adenoviral p53 transfection significantly increased cytotoxicity, which was additive in cell lines with wild-type p53 and more than additive in cell lines with mutant p53. The combination of two modalities increased the apoptotic population by 14% in A172 cells and 20% in D54 MG cells, which were the sum of apoptosis from each modality. Adenoviral p53 transfection increased the G1 phase fraction and concomitant decrease of radioresistant S phase fraction in A172 and D54MG cells. Our study demonstrated that p53 gene transfer combined with irradiation increased absolute cytotoxicity in human glioma cells used in this experiment. The interaction mechanism for increased cytotoxicity involved, in part, increased apoptosis and change of cell cycle profile.

Adenoviridae↗
PubMed1997

[Foreign bodies of the ear].

The paper presents practical aspects of diagnosis and management of foreign bodies found in the ear. Methodological approaches of medical aid to patients with foreign bodies in the ear are described. It is shown that such foreign bodies can provoke severe complications. The authors have developed a convenient classification which enables valid assessment of the clinical situation and facilitates a choice of the adequate therapeutic policy.

Adult↗
PubMed1993

[Differentiated treatment of generalized peritonitis].

The article discusses the results of treatment of 327 patients with generalized peritonitis in the toxic and terminal stages. The patients were divided conditionally into three groups. In the first group (42 patients) treatment consisted in routine cleansing of the abdominal cavity with drainage and introduction of antibiotics into it in the postoperative period. Mortality was 45.7%, complications occurred in 52.6% of patients who had survived. In the second group (145 patients) peritoneal dialysis in the flow-drip regimen was applied 3 or 4 times per 24 hours in a volume of 3-4 liters. In this group mortality was 37.9%, the number of complications among the survivals was 41.1%. In the third group (140 patients) a complex of differentiated peritoneal therapy was employed, including the following developed methods: thorough intraoperative cleansing of the abdominal cavity with 10-15 liters of antiseptic solutions containing antibiotics chosen according to the results of express-bacterioscopy, "active" peritoneal dialysis in the flow-fraction regimen, as well as developed methods of laparostomy--controlled laparostomy, prolonged open administration with local cleansing and use of antibacterial ointments. With use of this complex, mortality reduced to 27.1% and the incidence of complications to 20.6%.

Acute Disease↗
PubMed1990

[Mechanical jaundice: laparotomy or endoscopy?].

The results of diagnosis and surgical and endoscopic treatment in 285 patients with jaundice are analysed. The diagnostic-therapeutic tactics were divided into 3 periods. In the first period the diagnosis was based on the clinical findings and the results of laboratory biochemical tests. The postoperative death rate in this period was 15.3%. The second and third periods were characterized by early complex use of endoscopic diagnosis and treatment, which made it possible to reduce significantly the number of preoperative diagnostic errors and postoperative death rate. In the second period it was 5.9%, while in the third period there were no fatal outcomes. It is concluded that early endoscopic diagnosis and treatment in cases with obstructive jaundice is advisable.

Adult↗
PubMed1986

[Hemodynamic indices in refractory anemia subsequent to lung resection].

Results of the investigation of hemodynamics in 208 patients three days after resection of the lungs are presented. Reduction of the stroke volume and cardiac output was established as well as increased respiration energy, expansion of fluid volume which increased with reduction of oxygen capacity of blood despite the unaltered blood volume and were independent of the volume of surgery. To prevent these alterations of special significance are the restoration of oxygen blood capacity with the help of hemotransfusions.

Adult↗