[Conception of healthy nutrition].
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Biomedical subjects
Publications and source records attributed to I A Rogov.
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Contents of active factor of blood vessel growth angiogenin was analyzed in cow's milk by the method of competitive test in system "pancreatic RNAase/placental inhibitor of RNAase-angiogenin". High level of RNAase in milk limits using this test. To avoid this limitation the method of RNAase elimination from milk was elaborated.
Polyclonal antibodies to a milk antigen were obtained by a standard immunization procedure. The possibility was demonstrated to use the peroxidase-antibody conjugate in a competitive immunoenzymatic test. The minimal amount of the antigen determined by this method is 10 ng/ml.
Biotechnology is a prominent example of the interdisciplinary field of science based on the biology, biochemistry, microbiology, gene engineering, colloid chemistry and the totality of other sciences. The biotechnology includes a biotechnique which allow realisation of the resolutions of the engineering issues in the specific fields of science such as gene engineering, enzyme, cell, immune and microbiology technique. The biotechnological concepts are used to best advantage in the agriculture for the design and development of embryonic and gene-cell methods of animals. The biotechnology is also widely in chemical, pharmaceutical, food, oil-refinery and gas industries for the biotechnological conversion of the matter. It is applied in a power industry during the production of the bioenergy by photosynthesis, in the ecology for the microbiological destruction of water, treatment of sewage and in medicine for the production of antibiotics, hormones, amino acids ets. The biotechnology occupies the distinctive place in the food productions, in this context the analysis of the trends of its perspective development is reviewed.
The effect of food fibres of wheat bran, beet pectin and methylcellulose on the biological value of meat proteins has been studied in experiments on the growing rats. It has been shown, that up to 1% on food fibres of wheat bran and up to 0.5% of pectin and methylcellulose can be added to meat products without spoiling their qualitative indices. Higher levels of these substances cause the reduction of the biological value of meat products.
The general supremacy of classical theory of balanced nutrition entailed the tendency to remove ballast substances from food products. However, a new theory of adequate nutrition grounded a significant role of these substances in human ration. On the basis of experimental observations and theoretical theses of a new theory it was determined that animal connective tissue elements can be considered as ballast substances, that are physiologically useful for human organism. Proceeding from this a conclusion was made that a definite content of connective tissue in meat products does not reduce, but raises their nutritional value. A new level of knowledge made it possible to propose the modified method of integral score for food products nutritional value estimation.
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Properties of oil/water emulsions stabilized with the soluble casein-acid polysaccharide mixtures were investigated. Compared to initial protein solutions, higher emulsifying properties of the mixtures are demonstrated. A study is made on the influence of the properties of the mixtures for obtaining thermostable emulsions of hard consistency which could be applied in production of a great variety of foodstuffs. The role of the formation of protein-acid polysaccharide complexes is discussed.
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The influence of connective tissue on the biological value of meat products, manufactured from hashed meat, was studied in experiments on growing rats. It was shown that collagen level increase from 6.1 to 14.5% of the total amount of meat proteins improved the growth-mass characteristics of the animals. The actual biological value and the absolute protein utilization had a tendency to lowering, although the difference was not statistically significant. At the same time the effectiveness of tissue protein biosynthesis was not lowered. A conclusion has been made that the results of animal experiments correlate with the clinical data on the positive effect of the increase up to 15% (by protein) of collagen content on the anabolic action of hashed meat protein in humans. A further increase of the connective tissue amount leads to its diminished biological value.
The authors have considered calculating methods permitting design of fatty-acid composition of new full-value foodstuffs basing on combined utilization of varying types of raw materials, proceeding from the comparative analysis of the composition of the starting ingredients and hypothetic reference fat products. Certain regularities associated with the design of combined fat products have been confirmed. The lipid effectiveness coefficient values have been presented for a number of traditional and purified foodstuffs.
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A new method for the purification of bovine angiogenin is proposed which is based on the use of CM-cellulose, CM-Toyopearl, and Butyl-Toyopearl. Electrophoretically homogeneous protein was obtained with a 49% yield and 12,000-fold purification.