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Biomedical subjects

I Alexander

Publications and source records attributed to I Alexander.

9 recordsLinked to original sources
PubMed1987

Subcortical arteriosclerotic encephalopathy: Binswanger's disease.

Binswanger, in his 1894 dissertation on the differential diagnosis of general paresis of the insane, described a slowly progressive dementia associated with macroscopic loss of white matter. In recent years interest in Binswanger's disease was rekindled with CT demonstration of extensive white matter low densities in some patients. To define the clinical spectrum, we reviewed 22 consecutive cases in which the CT appearances suggested a diagnosis of Binswanger's disease. Two patients had focal neurological deficits at presentation, but recent anoxic or hypoglycaemic insults could not be excluded as the cause of the CT abnormalities. The 20 remaining patients were demented and showed variable combinations of corticobulbar dysfunction and gait dyspraxia. The duration of symptoms ranged from a few months to several years. Sixty per cent of this group gave a history of discrete stroke events and focal cortical and/or lacunar infarcts were a frequent CT finding. Binswanger's disease is probably due to chronic or acute-on-chronic white matter ischaemia. The association with lacunar and cortical infarctions suggests that a combination of large and small vessel disease produces diffuse ischaemia maximal in white matter watershed zones. Binswanger's disease is clinically differentiated from multi-infarct dementia by its time course.

Aged↗
PubMed1985

P1-purinoceptors mediate relaxation of the bovine bronchial artery.

Adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), adenosine 5'-monophosphate (AMP) and adenosine all relaxed the bronchial artery. All four purines tested were more efficacious than isoproterenol. Dipyridamole (10(-6) M) enhanced the relaxations due to adenosine and ATP while theophylline (10(-6) M) inhibited the relaxations due to adenosine and ATP. The results suggest the presence of P1-type purinoceptors in the bronchial artery of cattle.

Adenosine↗
PubMed1985

Comparison of ELISA with virus isolation for the diagnosis of genital herpes.

An enzyme linked immunosorbent assay (ELISA) system which detects and simultaneously types herpes simplex virus antigens in clinical specimens from patients with genital herpes has been compared with standard tissue culture isolation. Although more sensitive than a similar method previously described and also more sensitive than electron microscopy and immunofluorescence, ELISA did not detect all the viruses isolated in tissue culture. Costs were comparable. The speed of obtaining the result together with knowledge of the type causing infection are useful when antiviral chemotherapy is envisaged and when considering the likelihood of recurrences.

Antigens, Viral↗
PubMed1985

Evaluation of a genus reactive monoclonal antibody in rapid identification of Chlamydia trachomatis by direct immunofluorescence.

This study compared the direct visualisation of elementary bodies in urogenital smears by the direct immunofluorescence test with the isolation of Chlamydia trachomatis in McCoy cell cultures treated with cytochalasin B. C trachomatis was isolated from 41 (16.4%) of 250 unselected specimens in McCoy cell cultures treated with cytochalasin B. Of the 41 culture positive specimens 37 (90%) were diagnosed by direct visualisation of elementary bodies in the smears using the direct immunofluorescence test. Four specimens were positive by isolation only and a further seven were positive by the direct immunofluorescence test only. Overall the direct immunofluorescence test had a sensitivity of 90% and a specificity of 97%. The presence of red blood cells or mucus or the use of barrier creams did not appear to interfere with the normal visualisation of elementary bodies using the direct immunofluorescence test.

Antibodies, Monoclonal↗
PubMed1975

Epithelial surfaces of the trachea and principal bronchi in the rat.

The epithelial surfaces in the trachea and principal bronchi of healthy rats were examined by scanning electron microscopy. A system of four cell types, ciliated, microvillous, brush, and goblet cells, in this order of frequency, were found and intermediate type cells were not seen. An extensive area of the surface examined was covered by densely ciliated epithelium. The presence of other cell types beneath the cilia was confirmed by transmission electron microscopy. Areas up to 1 mm in diameter and randomly distributed were observed where microvillous cells predominated and only occasional ciliated cells were found. Most ciliated cells in these areas were adjacent to glandular openings or goblet cells. The larger microvilli of the brush cells were arranged in a coronal configuration elucidated by the scanning electron microscope. Preparatory techniques recently introduced for the examination of soft tissue in the scanning electron microscope facilitated the confirmation of cell types present and the microarchitecture of the epithelial surface.

Animals↗